Article
Biochemistry & Molecular Biology
Sifan Sun, Xu Li, Shaopei Gao, Nan Nie, Huan Zhang, Yufeng Yang, Shaozhen He, Qingchang Liu, Hong Zhai
Summary: In this study, a WRKY gene (ItfWRKY70) was isolated from a wild relative of sweet potato and found to play a positive role in drought tolerance by regulating stomatal aperture and activating the ROS scavenging system in sweet potato.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Wei Ma, Jie Li, Fengjun Liu, Tianzhen Zhang, Xueying Guan
Summary: In this study, the researchers found that suppressing GhHSP24.7 in cotton seedlings increases tolerance to heat and drought stress. GhHSP24.7 regulates the activity of lysine deacetylase in mitochondria, affecting the modification of mitochondrial proteins and reducing leaf ATP content, leading to the inability of GhHSP24.7 transgenic lines to coordinate stomatal closure under stress.
Article
Plant Sciences
Bailin Duan, Xiaofang Xie, Yanhua Jiang, Ning Zhu, Hongli Zheng, Yuxin Liu, Xuejun Hua, Yanyan Zhao, Yuqiang Sun
Summary: GhMYB44 gene plays a crucial role in plant response to abiotic stress, but its function in cotton is not well understood. In this study, GhMYB44 was induced by drought and ABA treatment in cotton. Silencing GhMYB44 resulted in physiological changes, including increased malondialdehyde content and decreased SOD activity. The silenced plants also exhibited increased stomatal aperture, water loss rate, and decreased drought tolerance.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Wei Jiang, Tao Tong, Wen Li, Zhenghong Huang, Guang Chen, Fanrong Zeng, Adeel Riaz, Hanna Amoanimaa-Dede, Rui Pan, Wenying Zhang, Fenglin Deng, Zhong-Hua Chen
Summary: This study investigates the molecular evolution, expression pattern, and physiological functions of 14-3-3 proteins in response to drought stress. The results demonstrate the highly conserved and early evolution of 14-3-3s in green plants and the duplication and expansion of 14-3-3s in angiosperms. The findings also highlight the crucial role of Hv14-3-3s in the rapid stomatal response to drought in barley. This research provides valuable evolutionary and molecular evidence for breeding drought-tolerant crops in a changing global climate.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Mengxiang Yang, Jiawei He, Zhuangzhuang Sun, Qing Li, Jian Cai, Qin Zhou, Bernd Wollenweber, Dong Jiang, Xiao Wang
Summary: Stomata play a crucial role in regulating plant growth by balancing photosynthesis and transpiration, especially in response to abiotic stress like drought. This study examined the stomatal behavior in intact wheat plants during the drought priming process using a portable microscope and non-invasive micro-test technology. The results showed that primed plants closed and reopened stomata faster than non-primed plants under drought stress and post-drought recovery, which was associated with higher accumulation of ABA and Ca2+ and activation of specific genes and ion channels. Overall, this research contributes to a better understanding of the stomatal response to drought priming and its impact on plant drought tolerance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Jin Hu, Mudan Luo, Xianming Zhou, Zhaoyang Wang, Li Yan, Dengfeng Hong, Guangsheng Yang, Xiaohui Zhang
Summary: This study identified a novel regulatory gene, BnaJUL1, and its target gene BnaTBCC1, which play important roles in drought tolerance in rapeseed. The findings provide valuable germplasm for breeding drought-tolerant rapeseed.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Zhonghua Bian, Yu Wang, Xiaoyan Zhang, Steven Grundy, Katherine Hardy, Qichang Yang, Chungui Lu
Summary: Light, especially green light, plays a significant role in enhancing drought tolerance in tomato plants through regulating stomatal aperture and ABA accumulation. The molecular responses of tomato plants under monochromatic red, blue, and green light spectrum with drought stress revealed the involvement of various genes related to plant hormone signal transduction, phototransduction, and calcium signaling pathway. Transcription factors like HY5, WRKY46, and WRKY81 were identified as key regulators in green light-induced drought responses.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Jialiang Sun, Hua Cui, Bingjie Wu, Weipeng Wang, Qiuyue Yang, Yaxin Zhang, Song Yang, Yuping Zhao, Dongbei Xu, Guoxiang Liu, Tengfei Qin
Summary: This study provides a comprehensive analysis of the GPDH gene family in cotton and identifies GhGPDH5 as a key player in drought stress and potentially involved in stomatal closure movements. These findings contribute to a fundamental understanding of GPDH genes in cotton.
Article
Plant Sciences
Dong Luo, Jie Liu, Yuguo Wu, Xi Zhang, Qiang Zhou, Longfa Fang, Zhipeng Liu
Summary: In this study, MsNTF2L was identified as a nucleus-, cytoplasm-, and plasma membrane-localized protein that plays an important role in drought tolerance in alfalfa. Overexpression of MsNTF2L resulted in hypersensitivity to ABA but enhanced tolerance to drought stress by reducing ROS levels and increasing stress/ABA-inducible gene expression. MsNTF2L also affected stomatal density, ABA-induced stomatal closure, and epicuticular wax accumulation, thus modulating leaf water loss. Moreover, transcript profiling revealed differential drought responses in genes related to stress/ABA signaling, antioxidant defense, and photosynthesis.
Article
Biochemistry & Molecular Biology
Amparo Rodriguez-hoces de la Guardia, Maria Beatriz Ugalde, Valeria Lobos-Diaz, Jesus Lucina Romero-Romero, Carlos Meyer-Regueiro, Claudio Inostroza-Blancheteau, Marjorie Reyes-Diaz, Felipe Aquea, Patricio Arce-Johnson
Summary: The study identified the homologous gene SIMYB60 of MYB60 in tomato plants, which is mainly expressed in leaves and repressed by abscisic acid. This gene potentially offers a molecular target for crop improvement by complementing the mutant phenotype in Arabidopsis.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Peipei Jiao, Yuanlin Liang, Shaoping Chen, Yang Yuan, Yongqiang Chen, Honghong Hu
Summary: Manipulation of the Bna.EPF2 gene in Brassica napus reduces stomatal density and size, leading to lower transpiration and improved water-use efficiency and drought tolerance without affecting photosynthesis or yield-related traits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chang-Heng Hsieh, Chia-Cheng Chou, Ya-Ching Fang, Po-Hao Hsu, Yi-Hung Chiu, Chi-Sheng Yang, Guey-Mei Jow, Chih-Yung Tang, Chung-Jiuan Jeng
Summary: Endogenous 14-3-3 proteins play a chaperone-like role in regulating the stability of Eag1 protein and modulators of 14-3-3 have therapeutic potential in correcting protein expression of disease-causing Eag1 mutants.
CELL AND BIOSCIENCE
(2023)
Article
Plant Sciences
Anja Petek-Petrik, Peter Petrik, Laurent J. Lamarque, Herve Cochard, Regis Burlett, Sylvain Delzon
Summary: The temporal variability of experimental drought mortality in conifers is best explained by an integrated trait that accounts for the rate at which the stomatal safety margin is crossed: the stomatal margin retention index. The regulation of water loss and the spread of xylem embolism have mostly been considered separately. The development of an integrated approach taking into account the temporal dynamics and relative contributions of these mechanisms to plant drought responses is urgently needed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Wenbo Chai, Nannan Song, Anqi Su, Jun Wang, Weina Si, Beijiu Cheng, Haiyang Jiang
Summary: MicroRNAs play a crucial role in regulating gene expression by targeting mRNA cleavage in plants, with a novel drought-responsive miRNA ZmmiR190 identified in maize. Overexpression of ZmmiR190 in Arabidopsis increased sensitivity to drought, while its likely target gene ZmCRP04 was shown to enhance drought tolerance in both Arabidopsis and rice. These findings suggest a potential molecular mechanism for improving crop drought tolerance through the ZmmiR190-ZmCRP04 pathway.
Review
Plant Sciences
Jose M. Mulet, Rosa Porcel, Lynne Yenush
Summary: Potassium is essential for the maintenance of cellular functions and viability in plant cells. Plants can regulate potassium fluxes to adapt to environmental changes and respond to stress. Understanding the molecular mechanisms of potassium homeostasis can be used to improve potassium transport efficiency and develop crops with enhanced abiotic stress tolerance through genetic engineering or breeding techniques.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Mingkee Achom, Proyash Roy, Beatriz Lagunas, Emma Picot, Luke Richards, Roxanna Bonyadi-Pour, Alonso J. Pardal, Laura Baxter, Bethany L. Richmond, Nadine Aschauer, Eleanor M. Fletcher, Monique Rowson, Joseph Blackwell, Charlotte Rich-Griffin, Kirankumar S. Mysore, Jiangqi Wen, Sascha Ott, Isabelle A. Carre, Miriam L. Gifford
Summary: A nodule circadian clock coordinates metabolic and regulatory activity during symbiosis by involving the gene Late Elongated Hypocotyl and rhythmic expression of nodule-specific cysteine-rich peptides. The rhythmic expression of these peptides may play a vital role in temporal coordination between bacterial activity and the rhythms of the plant host.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Liangliang Yu, Qi Di, Danping Zhang, Yumin Liu, Xiaolin Li, Kirankumar S. Mysore, Jiangqi Wen, Junhui Yan, Li Luo
Summary: PSK-delta, a novel legume-specific phytosulfokine, promotes symbiotic nodulation by enhancing nodule organogenesis.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Thomas B. Irving, Sanhita Chakraborty, Sergey Ivanov, Michael Schultze, Kirankumar S. Mysore, Maria J. Harrison, Jean-Michel Ane
Summary: This study characterized the symbiotic phenotypes of four Medicago truncatula mutants and identified two genes, RAM1 and KIN3, involved in mycorrhization. The results showed that RAM1 acts upstream of KIN3 and that KIN3 is involved in suppressing plant defenses and promoting root colonization. KIN3 also plays an essential role in the symbiotic response to soil nitrogen levels.
Article
Plant Sciences
Paolo M. Triozzi, Thomas B. Irving, Henry W. Schmidt, Zachary P. Keyser, Sanhita Chakraborty, Kelly Balmant, Wendell J. Pereira, Christopher Dervinis, Kirankumar S. Mysore, Jiangqi Wen, Jean-Michel Ane, Matias Kirst, Daniel Conde
Summary: Most legumes can establish a symbiotic relationship with soil rhizobia, promoting the formation of root nodules for efficient nitrogen fixation. Coordination of nodule organogenesis and rhizobial infection in space and time is crucial for successful nodulation. The plant hormone cytokinin (CK) plays a key role as a positive regulator in nodule organogenesis, but the temporal regulation of tissue-specific CK signaling and biosynthesis in response to bacterial lipo-chitooligosaccharides (LCOs) or Sinorhizobium meliloti inoculation remains poorly understood.
Article
Plant Sciences
Bikram D. Pant, Seonghee Lee, Hee-Kyung Lee, Nick Krom, Pooja Pant, YoonJeong Jang, Kirankumar S. Mysore
Summary: The overexpression of the Arabidopsis small GTPase nucleolar GTP-binding proteins enhances drought tolerance in rice by regulating the formation of silica bodies around stomata to reduce water loss.
Article
Multidisciplinary Sciences
Vidhyavathi Raman, Clemencia M. Rojas, Balaji Vasudevan, Kevin Dunning, Jaydeep Kolape, Sunhee Oh, Jianfei Yun, Lishan Yang, Guangming Li, Bikram D. Pant, Qingzhen Jiang, Kirankumar S. Mysore
Summary: This study developed a strategy to increase the efficiency of plant transformation by engineering Agrobacterium and suppressing host defense responses. The results showed that the transformation efficiency of wheat, alfalfa and switchgrass increased by 250%-400% using the engineered Agrobacterium.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Plant Sciences
Urooj Fatima, Anjali Anjali, Muthappa Senthil-Kumar
TRENDS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Wolf-Rudiger Scheible, Pooja Pant, Bikram D. Pant, Nick Krom, Randy D. Allen, Kirankumar S. Mysore
Summary: Phosphorus limitation induces the expression of new genes, including previously unannotated genes, non-coding RNAs, small peptides, and alternatively spliced RNAs, and enhances disease and drought tolerance.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Letter
Biochemistry & Molecular Biology
Yingying Meng, Chongnan Wang, Qiqi Li, Wenkai Ji, Jiangqi Wen, Kirankumar S. Mysore, Yanxi Pei, Lifang Niu, Hao Lin
JOURNAL OF GENETICS AND GENOMICS
(2023)
Editorial Material
Biochemistry & Molecular Biology
Aanchal Choudhary, Muthappa Senthil-Kumar
Summary: This article highlights recent important discoveries on effector-mediated manipulation of apoplast hydration and the involvement of ABA machinery in later stages of bacterial infection leading to stomatal closure. It also summarizes the differences between early and later stages of infection, where COR signaling promotes stomatal opening in the initial phase while ABA signaling dominates and causes stomatal closure in later stages. Furthermore, it provides an overview of the current understanding of pathogen-driven modulation of leaf water status during infection, with stomata playing a crucial role in the post-invasive stage.
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhenpeng Luo, Jiang Wang, Fuyu Li, Yuting Lu, Zijun Fang, Mengdi Fu, Kirankumar S. Mysore, Jiangqi Wen, Jiming Gong, Jeremy D. Murray, Fang Xie
Summary: Legumes regulate nodulation through the expression and regulation of MtNRT2.1, MtNLP1, and MtCEP1. MtNRT2.1 affects nodulation differently in low- and high-nitrate conditions, with MtNLP1 promoting its expression under low-nitrate conditions and suppressing it under high-nitrate conditions. MtCEP1 independently enhances nodulation by increasing the expression of NRT2.1 under low-nitrate conditions.
Article
Plant Sciences
Fathi Berrabah, Gautier Bernal, Ait-Salem Elhosseyn, Cyrille El Kassis, Roxane L'Horset, Farouk Benaceur, Jiangqi Wen, Kirankumar S. Mysore, Marie Garmier, Benjamin Gourion, Pascal Ratet, Veronique Gruber
Summary: This study investigated the relationship between senescence and immunity in Medicago nodules. It found that in nonfunctional nodules, senescence and immunity appear to be antagonistic, while in normal nodules under stress, they switch to a synergistic relationship. The study also identified key genes involved in symbiotic immunity suppression. Understanding the links between senescence and immunity in nodules contributes to the knowledge of nodule organogenesis.
Article
Plant Sciences
Ru-Jie Li, Chun-Xiao Zhang, Sheng-Yao Fan, Yi-Han Wang, Jiangqi Wen, Kirankumar S. Mysore, Zhi-Ping Xie, Christian Staehelin
Summary: This study reveals the presence of a second Nod factor cleaving hydrolase (MtCHIT5b) in Medicago truncatula, which degrades Sinorhizobium meliloti Nod factors in the rhizosphere in conjunction with MtNFH1. The expression of MtCHIT5b is induced by purified Nod factors treatment or rhizobia inoculation, and it is localized in the infection pocket of root hairs. The study also demonstrates that overexpression of MtCHIT5b leads to reduced nodule formation, and simultaneous silencing of MtCHIT5b and MtNFH1 results in decreased nodulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Urooj Fatima, D. Balasubramaniam, Wajahat Ali Khan, Manu Kandpal, Jyothilakshmi Vadassery, Arulandu Arockiasamy, Muthappa Senthil-Kumar
Summary: AtSWEET11 and AtSWEET12 are important sucrose efflux transporters in Arabidopsis that play synergistic roles in apoplasmic phloem loading, seed filling, and sugar level alteration during pathogen infection. Structural analysis revealed that these transporters bind sucrose in the central cavity and transmembrane region, providing alternate access for transport from either side of the membrane. Furthermore, they are also involved in plant development and in responses to abiotic and biotic stresses.
Article
Plant Sciences
Nilesh Kumar Sharma, Shalini Yadav, Santosh Kumar Gupta, Vadivelmurugan Irulappan, Aleena Francis, Muthappa Senthil-Kumar, Debasis Chattopadhyay
Summary: Plants respond to drought and pathogen attacks by depositing lignin in the secondary cell wall. In chickpea roots, the expression of several LAC genes is upregulated while microRNA397 (CamiR397) is downregulated in response to natural drought. CamiR397 targets LAC4 and LAC17L genes in chickpea roots. Overexpression of CamiR397 reduces lignin deposition and thickness of the xylem wall in chickpea roots. Downregulation of CamiR397 increases lignin deposition in chickpea roots. Furthermore, CamiR397-overexpressing and STTM397 chickpea lines show sensitivity and tolerance, respectively, to the fungal pathogen Macrophomina phaseolina, which causes dry root rot (DRR) disease in chickpea. This study highlights the regulatory role of CamiR397 in root lignification during drought and DRR in chickpea.
PLANT CELL AND ENVIRONMENT
(2023)