期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 15, 期 11, 页码 1387-1396出版社
WILEY
DOI: 10.1111/pbi.12723
关键词
multiple disease resistance; Lr34; rust; anthracnose; flavonoid phytoalexin
资金
- Bill and Melinda Gates foundation [OPP1060218, OPP1131636]
- Research Grants Council of Hong Kong, China [17 123 315]
- Bill and Melinda Gates Foundation [OPP1060218, OPP1131636] Funding Source: Bill and Melinda Gates Foundation
The ability of the wheat Lr34 multipathogen resistance gene (Lr34res) to function across a wide taxonomic boundary was investigated in transgenic Sorghum bicolor. Increased resistance to sorghum rust and anthracnose disease symptoms following infection with the biotrophic pathogen Puccinia purpurea and the hemibiotroph Colletotrichum sublineolum, respectively, occurred in transgenic plants expressing the Lr34res ABC transporter. Transgenic sorghum lines that highly expressed the wheat Lr34res gene exhibited immunity to sorghum rust compared to the low-expressing single copy Lr34res genotype that conferred partial resistance. Pathogen-induced pigmentation mediated by flavonoid phytoalexins was evident on transgenic sorghum leaves following P. purpurea infection within 24-72 h, which paralleled Lr34res gene expression. Elevated expression of flavone synthase II, flavanone 4-reductase and dihydroflavonol reductase genes which control the biosynthesis of flavonoid phytoalexins characterized the highly expressing Lr34res transgenic lines 24-h post-inoculation with P. purpurea. Metabolite analysis of mesocotyls infected with C. sublineolum showed increased levels of 3-deoxyanthocyanidin metabolites were associated with Lr34res expression, concomitant with reduced symptoms of anthracnose.
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