The Constitutive Endopolygalacturonase TvPG2 Regulates the Induction of Plant Systemic Resistance by Trichoderma virens

Trichoderma spp. are opportunistic fungi some of which are commonly present in the rhizosphere. Several species, such as T virens, are also efficient biocontrol agents against phytopathogenic fungi and exert beneficial effects on plants. These effects are the consequence of interactions between Trichoderma and plant roots, which trigger enhanced plant growth and induce plant resistance. We have previously shown that T virens I10 expresses two endopolygalacturonase genes, tvpg1 and tvpg2, during the interaction with plant roots; tvpgl is inducible while tvpg2 is constitutively transcribed. Using the same system, the tomato polygalacturonase-inhibitor gene Lepgip1 was induced at the same time as tvpgl. Here we show by gene disruption that TvPG2 performs a regulatory role on the inducible tvpgl gene and in triggering the plant immune response. A tvpg2-knockout strain fails to transcribe the inducible tvpgl gene in neither in vitro in inducing media containing pectin or plant cell walls, nor during the in vivo interaction with tomato roots. Likewise, the in vivo induction of Lepgip1 does not occur, and its defense against the pathogen Botrytis cinerea is significantly reduced. Our data prove the importance of a T virens constitutively produced endopolygalacturonase in eliciting plant induced systemic resistance against pathogenic fungi.