期刊
PHYSIOLOGICAL GENOMICS
卷 49, 期 1, 页码 53-66出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/physiolgenomics.00083.2016
关键词
physical activity; RNA-Seq; heart; connective tissue growth factor; cardiorespiratory fitness; (V)over dotO(2peak)
资金
- College of Veterinary Medicine at the University of Missouri [AHA 16PRE2715005]
- University of Missouri Life Sciences Fellowship Program
Peak oxygen consumption (V)over dotO(2peak)) strongly predicts morbidity and mortality better than other established risk factors, yet mechanisms associated with its age-associated decline are unknown. Our laboratory has shown that (V)oevr dotO(2peak) first begins to decrease at the same age of 19-20 wk in both sedentary and wheel-running, female Wistar rats (Toedebusch et al., Physiol Genomics. 48: 101-115, 2016). Here, we employed a total systemic approach using unsupervised interrogation of mRNA with RNA sequencing. The purpose of our study was to analyze transcriptomic profiles from both sedentary (SED) and wheel-running (RUN) conditions as a strategy to identify pathways in the left ventricle that may contribute to the initial reductions in (V)over dotO(2peak) occurring between 19 and 27 wk of age. Transcriptomic comparisons were made within both SED and RUN rats between 19 and 27 wk (n = 5-8). Analysis of mRNAs shared in SED and RUN between 19 and 27 wk found 17 upregulated (e.g., Adra1d, Rpl17, Xpo7) and 8 downregulated (e.g., Cdo1, Ctfg, Sfrp1) mRNAs, at 19 wk, respectively. Furthermore, bioinformatics analysis of mRNAs common to SED and RUN produced networks suggestive of increased connective tissue development at 27 vs. 19 wk. Additionally, Ctfg mRNA was negatively associated with (V)over dotO(2peak) in both SED and RUN (P < 0.05). In summary, transcriptomic analysis revealed mRNAs and networks associated with increased connective tissue development, decreased alpha-adrenergic activity, and decreased protein translation in the left ventricle that could, in part, potentially influence the initiation of the left ventricle that could, in part, potentially influence the initiation of the lifelong reduction in (V)over dotO(2peak), independent of physical activity levels.
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