期刊
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
卷 19, 期 24, 页码 16257-16266出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cp07774a
关键词
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资金
- [15K18518]
- [25870407]
- [15K07038]
- Grants-in-Aid for Scientific Research [17K07362, 16H00836, 15K07038] Funding Source: KAKEN
We herein report the mechanism of amyloid formation of amyloid-beta (A beta) peptides on small (SUV) and large unilamellar vesicles (LUVs), which consist of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipids. Although A beta(1-42) formed fibrils on SUVs at all POPC concentrations used, the lag time, elongation rate, maximum thioflavin T intensity, and fibrillar morphology were distinct, indicating polymorphic amyloid formation. LUVs, at low POPC concentrations, did not markedly affect fibrillation kinetics; however, increases in POPC concentrations suppressed amyloid formation. No significant differences in the thermal stabilities of A beta(1-42) fibrils formed with and without vesicles were observed, although fibrils formed on SUVs showed some differences with dilution. SUVs markedly promoted A beta(1-40) fibrillation by condensing A beta(1-40), whereas no effects of LUVs on amyloidogenesis were detected. Salts greatly increased A beta(1-40) amyloidogenicity on vesicles. We proposed comprehensive models for vesicle size-dependent A beta amyloidogenesis. Inhomogeneous packing defects in SUVs may induce distinct nucleation in the polymorphisms of amyloids and decreasing local concentrations of Ab with higher amounts of LUVs inhibits amyloid formation. We also pointed out that C-terminal hydrophobicity of A beta is important for amyloidogenesis on membranes.
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