Indirect NMR detection of transient guanosyl radical protonation in neutral aqueous solution

By using the time-resolved chemically induced dynamic nuclear polarization technique, we show that the neutral guanosyl radical, G(-H)(center dot), formed in the reaction of guanosine-5'-monophosphate with a triplet-excited 3,3',4,4'-tetracarboxy benzophenone in neutral aqueous solution, protonates readily at the N7 position with the formation of a new guanosyl cation radical (G(center dot+))'.