Article
Spectroscopy
Hongpeng Wang, Yingjian Xin, Xiong Wan
Summary: This paper reports on the spectral characterization of porphyrins and terpenoids in different vegetable oils, classifying them using a PCA-SVM model. The study analyzed the effects of oil types and processing technology on spectral differences, finding that chlorophyll and β-carotene were the main contributors to the fingerprint information in the spectral techniques. Extra virgin olive oil obtained by physical cold pressing had the highest content of porphyrins and terpenoids, indicating the strongest activity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Hanyu Liang, Hongjuan Chen, Xinya Liu, Zihan Wang, Pengfu Li, Shan Lu
Summary: This study aims to investigate the synthesis mechanism of astaxanthin and suggests that the hydroxylation step may be the bottleneck for heterologous production of astaxanthin in Synechocystis.
Article
Spectroscopy
V. S. Novikov, V. V. Kuzmin, M. E. Darvin, J. Lademann, E. A. Sagitova, K. A. Prokhorov, L. Yu Ustynyuk, G. Yu Nikolaeva
Summary: Using density functional theory (DFT), the study analyzed the structures and Raman spectra of trans-isomers of different carotenoids, finding that the position of the C=C stretching band is mainly determined by the number of conjugated C=C bonds and decreases with increased conjugation length. The C-C stretching band shifts with conjugation length or mass of end groups, and the presence of side CH3 groups affects the intensity and position of the bands.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Joy Udensi, Ekaterina Loskutova, James Loughman, Hugh J. Byrne
Summary: The study explored the differentiation and quantification of dietary carotenoids using Raman and UV visible absorption spectroscopy. By preprocessing spectral data and modeling analysis, accurate determination of carotenoid content was achieved, suggesting potential application in clinical analysis.
Article
Biotechnology & Applied Microbiology
Raquel Amaral, J. Sergio Seixas de Melo, Lilia Maria Antunes dos Santos
Summary: Eustigmatophytes exhibit a distinctive pigment profile containing chlorophylls and carotenoids. Violaxanthin is the most abundant carotenoid among this class, followed by beta,beta-carotene. Research suggests the potential of eustigmatophytes as natural sources of carotenoids.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Plant Sciences
Nitisha Bhargava, Charles Ampomah-Dwamena, Charlotte Voogd, Andrew C. Allan
Summary: This study analyzed the molecular mechanisms of carotenoid accumulation in different flesh-colored kiwifruit species. Orange-fleshed kiwifruits formed larger crystals of carotenoids in their chromoplasts, while yellow and green-fleshed kiwifruits mainly accumulated carotenoids in crystalline form. Metabolic profiles and gene expression levels revealed higher expression of upstream carotenoid biosynthesis-related genes in orange-fleshed kiwifruits compared to green-fleshed ones. These findings suggest coordinated changes in the carotenoid pathway and plastid type are responsible for the orange phenotype in certain kiwifruit species.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Joy Udensi, James Loughman, Ekaterina Loskutova, Hugh J. Byrne
Summary: This review discusses the structural and optical properties of carotenoid compounds and the use of Raman spectroscopy to identify carotenoids in biological environments. It focuses on applications in nutritional analysis, optometry, and serology, and explores the progress and challenges in establishing the technique in a clinical environment.
Article
Food Science & Technology
Gagan Jyot Kaur, Kitson Morden, Valerie Orsat, Ashutosh Singh
Summary: This study investigates the processing of carrot rejects and waste to produce carrot puree by mixing processed carrot discards and graded carrots. A novel processing method involving ultrasonication and mechanical homogenization is used to achieve efficient utilization of waste. The processed puree exhibits desirable particle size distribution, beta-carotene content, and color, comparable to puree samples processed without peels and crowns.
FOOD AND BIOPROCESS TECHNOLOGY
(2022)
Article
Food Science & Technology
Yanfei Liu, Guowen Lv, Jiaxin Chai, Yaqi Yang, Fengwang Ma, Zhande Liu
Summary: The study showed that 1-MCP treatment increased beta-carotene accumulation and decreased chlorophyll content in 'Qihong' kiwifruit, resulting in a more yellow flesh color. Ethylene response factors were found to play a role in regulating carotenoid and chlorophyll-related genes, impacting fruit coloration during the ripening stage.
Article
Chemistry, Applied
Peipei Fang, Hongpeng Wang, Xiong Wan
Summary: This study introduces a method using laser confocal Raman technique combined with theoretical Raman spectra based on DFT to accurately analyze the molecular differences between extra virgin olive oil (EVOO) and low-cost olive oil, and achieve quantitative analysis using chemometrics.
Article
Biotechnology & Applied Microbiology
Sanja Radman, Marina Zekic, Ivana Flanjak, Ana-Marija Cikos, Stela Jokic, Igor Jerkovic
Summary: The aim of this research was to contribute to the understanding of the chemodiversity of G. barbata and E. crinita, as well as the knowledge of protected Cystoseira areas. Different extraction methods were used to analyze the chemical profiles of these two algae, revealing significant variations and differences, which are important for their taxonomic classification.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Plant Sciences
Shihan Xing, Ruijie Li, Haoqiang Zhao, Hong Zhai, Shaozhen He, Huan Zhang, Yuanyuan Zhou, Ning Zhao, Shaopei Gao, Qingchang Liu
Summary: This study identified IbNAC29 as a potential candidate gene for improving the nutritional value of sweet potato. Overexpression of IbNAC29 increased the content of carotenoids in the storage roots and up-regulated the expression of carotenoid biosynthesis genes. Further investigation revealed that IbNAC29 interacted with IbMYB1R1 and IbAITR5 to inhibit the activity of IbSGR1, leading to the up-regulation of carotenoid biosynthesis gene IbPSY.
HORTICULTURE RESEARCH
(2023)
Article
Chemistry, Applied
Silvia Portarena, Chiara Anselmi, Luca Leonardi, Simona Proietti, Anna Rita Bizzarri, Enrico Brugnoli, Chiara Baldacchini
Summary: Carotenoids have a significant impact on the stability, freshness, and nutritional value of EVOO. A reliable method has been developed using Resonant Raman spectroscopy to quickly assess the lutein/beta-carotene ratio in EVOO, providing a fast and non-destructive approach for quality assessment and authenticity verification.
Article
Food Science & Technology
Wenyang Tao, Xingqian Ye, Yanping Cao
Summary: The study found that all-trans-beta-carotene undergoes degradation during oral, gastro, and intestinal digestion, with the most significant degradation occurring during intestinal digestion. Isomerization was observed during oral digestion, and different degradation products with various functional groups were identified.
FOOD SCIENCE AND HUMAN WELLNESS
(2021)
Article
Chemistry, Applied
C. Anselmi, S. Portarena, C. Baldacchini, S. Proietti, L. Leonardi, E. Brugnoli
Summary: This paper introduces a novel method using Raman spectroscopy for the rapid quantification of β-carotene in olive oil. By analyzing the ratio of lutein to β-carotene, the study reveals the health condition of olives before processing and provides a means for oil producers to evaluate the effectiveness of prevention strategies against olive fly attacks.
Article
Chemistry, Physical
Mindaugas Macernis, Simona Streckaite, Radek Litvin, Andrew A. Pascal, Manuel J. Llansola-Portoles, Bruno Robert, Leonas Valkunas
Summary: In this study, the absorption and vibrational properties of allene-containing carotenoids were experimentally investigated. The electronic properties of vaucheriaxanthin were successfully explained by calculations, while fucoxanthin and butanoyloxyfucoxanthin required long-range corrections to accurately determine their absorption position. Resonance Raman spectra of these carotenoids were perturbed by the presence of the allene group, which could be attributed to the increased Raman intensity of the C=C antisymmetric stretching mode in the carotenoid chain.
JOURNAL OF PHYSICAL CHEMISTRY A
(2022)
Article
Plant Sciences
Martina Beckova, Roman Sobotka, Josef Komenda
Summary: The repair of photosystem II is crucial for the functionality of oxygenic photosynthesis. The study shows that the existence of a stable no reaction centre complex (NRC) is not supported, as each unassembled module remains separate and does not form a mutual complex.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Andrew Hitchcock, Christopher Neil Hunter, Roman Sobotka, Josef Komenda, Marcel Dann, Dario Leister
Summary: The Perspective article discusses the plans of the PhotoRedesign consortium funded by the European Research Council to enhance photosynthesis by redesigning the light reactions. The goal is to improve the efficiency and resilience of photosynthesis by combining synthetic biology approaches and adaptive laboratory evolution to create a mechanism that can better utilize solar energy.
Article
Plant Sciences
Jana Knoppova, Roman Sobotka, Jianfeng Yu, Martina Beckova, Jan Pilny, Joko P. Trinugroho, Ladislav Csefalvay, David Bina, Peter J. Nixon, Josef Komenda
Summary: Analysis of isolated assembly complexes provides new insights into the early stages of photosystem II biogenesis. These complexes contain information about the PSII subunits and proteins and assembly factors associated with its assembly process.
Correction
Multidisciplinary Sciences
Petra Skotnicova, Hristina Staleva-Musto, Valentyna Kuznetsova, David Bina, Minna M. Konert, Shan Lu, Tomas Polivka, Roman Sobotka
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Ivo Sedlacek, Pavla Holochova, Hans-Jurgen Busse, Vendula Koublova, Stanislava Kralova, Pavel Svec, Roman Sobotka, Eva Stankova, Jan Pilny, Ondrej Sedo, Jana Smolikova, Karel Sedlar
Summary: A group of seven bacterial strains producing blue-purple pigmented colonies were isolated from freshwater samples collected in a deglaciated part of Antarctica. Through a comprehensive taxonomic approach, a new Massilia species was identified.
Meeting Abstract
Biochemistry & Molecular Biology
Maria Jose Saucedo Rodriguez, Petr Pecina, Kristyna Cunatova, Marek Vrbacky, Alena Pecinova, Roman Sobotka, Carlo Viscomi, Massimo Zeviani, Tomas Mracek, Josef Houstek
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Multidisciplinary Sciences
Eva Horakova, Laurence Lecordier, Paula Cunha, Roman Sobotka, Piya Changmai, Catharina J. M. Langedijk, Jan Van den Abbeele, Benoit Vanhollebeke, Julius Lukes
Summary: The decreased functionality and expression of HpHbR in Trypanosoma brucei gambiense is a key evolutionary modification that allows this parasite to infect humans. This study reveals that hemoglobin uptake in African trypanosomes is almost exclusively mediated by HpHbR and crucial for their development. Understanding this molecular mechanism is important for our knowledge of how the parasite infects humans and for the development of potential treatments.
NATURE COMMUNICATIONS
(2022)
Correction
Plant Sciences
Minna M. Konert, Anna Wysocka, Peter Konik, Roman Sobotka
PHOTOSYNTHESIS RESEARCH
(2023)
Article
Plant Sciences
Jaroslav Nisler, Zuzana Kucerova, Radoslav Koprna, Roman Sobotka, Jana Slivkova, Stephen Rossall, Martina Spundova, Alexandra Husickova, Jan Pilny, Danuse Tarkowska, Ondrej Novak, Maria Skrabisova, Miroslav Strnad
Summary: Increasing crop productivity and mitigating yield losses under stressful conditions are major challenges in agriculture. A recently discovered compound, MTU, delays senescence and enhances the growth of wheat plants. Multiyear field trials demonstrate that treatment with MTU increases average grain yields of wheat and barley by 5-8%. This compound acts independently of cytokinins or other phytohormones and appears to be the only chemical known to affect the stability and activity of PSI.
FRONTIERS IN PLANT SCIENCE
(2023)
Correction
Biochemistry & Molecular Biology
Mark J. J. Burton, Joel Cresser-Brown, Morgan Thomas, Nicola Portolano, Jaswir Basran, Samuel L. L. Freeman, Hanna Kwon, Andrew R. R. Bottrill, Manuel J. J. Llansola-Portoles, Andrew A. A. Pascal, Rebekah Jukes-Jones, Tatyana Chernova, Ralf Schmid, Noel W. W. Davies, Nina M. M. Storey, Pierre Dorlet, Peter C. E. S. Moody, John S. L. Mitcheson, Emma Raven
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Rameez Arshad, Francesco Saccon, Pushan Bag, Avratanu Biswas, Claudio Calvaruso, Ahmad Farhan Bhatti, Steffen Grebe, Vincenzo Mascoli, Moontaha Mahbub, Fernando Muzzopappa, Alexandros Polyzois, Christo Schiphorst, Mirella Sorrentino, Simona Streckaite, Herbert van Amerongen, Eva-Mari Aro, Roberto Bassi, Egbert J. Boekema, Roberta Croce, Jan Dekker, Rienk van Grondelle, Stefan Jansson, Diana Kirilovsky, Roman Kouril, Sylvie Michel, Conrad W. Mullineaux, Klara Panzarova, Bruno Robert, Alexander Ruban, Ivo van Stokkum, Emilie Wientjes, Claudia Buechel
Summary: The latest fundamental knowledge on light-harvesting mechanisms of antenna proteins and their application in biotechnical optimization of photosynthesis are discussed in this article. The dynamic nature of antenna complexes and their adaptation to different light environments are highlighted. High-resolution microscopy and spectroscopic studies provide insights into the interactions between antennae and other thylakoid membrane components.
Article
Plant Sciences
Vasco Giovagnetti, Marianne Jaubert, Mahendra K. Shukla, Petra Ungerer, Jean-Pierre Bouly, Angela Falciatore, Alexander Ruban
Summary: This study investigates the role of LHCX1 in nonphotochemical energy-dependent quenching (qE), revealing its mechanism in diatoms and its differences from other proteins such as LHCSR and S protein. Results suggest that LHCX1 may have evolved independently to play a crucial role in tracking light fluctuations in turbulent waters.
