Article
Immunology
Xufei Zhang, Magali Monnoye, Mahendra Mariadassou, Fabienne Beguet-Crespel, Nicolas Lapaque, Christine Heberden, Veronique Douard
Summary: The study revealed different impacts of fructose and glucose intake on mice, with glucose leading to increased adiposity, glucose intolerance, and intestinal permeability, while fructose resulted in elevated IL6 levels. The research also identified the potential roles of microbiota composition and inflammatory factors in gut barrier function and inflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Nutrition & Dietetics
Ikuya Shirai, Koji Karasawa, Yusuke Kodaira, Yu Iwasaki, Yasutaka Shigemura, Hidefumi Makabe, Shigeru Katayama
Summary: This study evaluated the permeation of agaro-oligosaccharides (AOSs) in the gastrointestinal tract both in vivo and in vitro. The results showed that AOSs can be absorbed through the paracellular pathway between intestinal epithelial cells.
FRONTIERS IN NUTRITION
(2022)
Article
Nutrition & Dietetics
Sandip Nathani, Neeladrisingha Das, Parul Katiyar, Bhairavnath Waghmode, Debabrata Sircar, Partha Roy
Summary: This study assessed the role of Indian mustard honey in preventing lipopolysaccharide-induced intestinal barrier dysfunction. The results showed that honey prevented LPS-induced intestinal barrier dysfunction in a dose-dependent manner, both in vitro and in vivo. The use of honey upregulated the cellular localization and expression of tight junction proteins, and downregulated the expression of pro-inflammatory cytokines.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Immunology
Mia Ogaard Monsted, Nora Dakini Falck, Kristina Pedersen, Karsten Buschard, Laurits Juulskov Holm, Martin Haupt-Jorgensen
Summary: Research on patients with T1D and animal models of T1D indicates a link between increased intestinal permeability and disease development, highlighting the importance of careful selection of research methods and experimental setups.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Gastroenterology & Hepatology
Nga Le, Claire Mazahery, Kien Nguyen, Alan D. Levine
Summary: The study reveals that communication between T cells and the intestinal epithelium can impact epithelial barrier function by regulating the assembly of tight junction complex. Activated T cells have different effects on ion flux and cell morphology, ultimately leading to cell death or disruption.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Cell Biology
Dipak Kumar Sahoo, Marilyn N. Martinez, Kimberly Dao, Vojtech Gabriel, Christopher Zdyrski, Albert E. Jergens, Todd Atherly, Chelsea A. Iennarella-Servantez, Laura E. Burns, Dwayne Schrunk, Donna A. Volpe, Karin Allenspach, Jonathan P. Mochel
Summary: To study the effects of drug-intestinal barrier interactions on therapeutic failure or toxicity, new methods are needed to evaluate drug-gut interactions in veterinary medicine. Three-dimensional (3D) organoids can provide a cost-effective system to address these difficulties without invasive in vivo assays. In this study, we demonstrate the growth and differentiation of canine-colonoid-derived intestinal epithelial cells in a 2D monolayer, and evaluate the permeability of passive beta-blockers in this canine-derived 2D model. The results show promising potential for species-specific assessments of drug permeability.
Article
Gastroenterology & Hepatology
F. Christopher Peritore-Galve, Izumi Kaji, Anna Smith, Lauren M. Walker, John A. Shupe, M. Kay Washington, Holly M. Scott Algood, Pradeep K. Dudeja, James R. Goldenring, D. Borden Lacy
Summary: Clostridioides difficile infection (CDI) is a leading cause of hospital-acquired diarrhea and pseudomembranous colitis. This study found that CDI increases intestinal permeability and decreases the abundance of key ion transporters, leading to osmotic diarrhea. These findings provide insights into the mechanism of diarrhea during CDI and may lead to new treatments for CDI-associated diarrhea.
Article
Biochemistry & Molecular Biology
Nanthakarn Woottisin, Sophida Sukprasert, Thitianan Kulsirirat, Thipaporn Tharavanij, Korbtham Sathirakul
Summary: This study investigated the permeability of rosmarinic acid (RA) in Thunbergia laurifolia (TL) extract and as a pure compound. The results showed that the permeability of RA significantly increased after deconjugation. RA was transported through Caco-2 cells via passive diffusion. RA in TL extract mainly penetrated as conjugated forms with glucuronic acid and/or sulfate.
Article
Pharmacology & Pharmacy
Milica Markovic, Moran Zur, Sapir Garsiani, Daniel Porat, Sandra Cvijic, Gordon L. Amidon, Arik Dahan
Summary: The study aimed to evaluate the intestinal permeability mechanisms of minoxidil, with a focus on paracellular transport, and determine its suitability as a reference drug for the Biopharmaceutics Classification System (BCS). The results indicated that paracellular transport plays a significant role in the intestinal permeability of minoxidil following oral administration.
Review
Biochemistry & Molecular Biology
Daniel Efrain Molotla-Torres, Fabiola Guzman-Mejia, Marycarmen Godinez-Victoria, Maria Elisa Drago-Serrano
Summary: The gut epithelium controls intestinal permeability through tight junction proteins. Stress disrupts this control by affecting the binding of stress hormones to receptors. Studying the neuroendocrine effects of stress and intestinal permeability is important for the treatment of related diseases.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yu Takahashi, Makoto Noguchi, Yu Inoue, Shintaro Sato, Makoto Shimizu, Hirotatsu Kojima, Takayoshi Okabe, Hiroshi Kiyono, Yoshio Yamauchi, Ryuichiro Sato
Summary: This study demonstrates that organoid-derived intestinal epithelial cells possess similar physiological properties as intestinal epithelium and can be used as tools to enhance the prediction of biological activity in humans.
Review
Gastroenterology & Hepatology
Ivana Roosevelt Sequeira
Summary: The importance of plasma ascorbic acid lies in its enzymatic and antioxidant properties, as well as its involvement in various aspects of health. Dietary intake supports optimal levels, with supplementation at higher doses being a potential option. High dose AA may transiently increase intestinal permeability to enhance delivery of other micronutrients, though further study is needed to understand the potential mechanism for increasing small intestinal nutrient co transport and absorption.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Biology
Weiyin Huang, Shuang Chen, Lin Sun, Hubin Wwang, Hongqun Qiao
Summary: The study investigated the intestinal permeability of lamivudine using Caco-2 cells monolayer and single-pass intestinal perfusion, revealing a passive diffusion absorption mechanism possibly related to efflux transporters. Lamivudine was classified as a moderate-permeability drug in the Biopharmaceutics Classification System.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Valentina Rizzo, Nadia Ferlazzo, Monica Curro, Gaetano Isola, Marco Matarese, Maria Paola Bertuccio, Daniela Caccamo, Giovanni Matarese, Riccardo Ientile
Summary: Studies have shown that Baicalin (BA) extracted from Scutellaria baicalensis root modulates autophagic signaling and inflammatory response in intestinal epithelial cells. BA treatment suppressed expression of inflammatory cytokines, restored claudin 1 levels, and reduced NF-κB activation induced by LPS, suggesting anti-inflammatory effects of BA through regulation of autophagic flux.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Tugba Gulsun, Nihan Izat, Selma Sahin
Summary: This study aimed to evaluate the effects of permeability enhancers on the permeability of furosemide and metformin hydrochloride. The results showed that permeability enhancers had no significant effect on the permeability of furosemide, but significantly increased the permeability of metformin. Combining chitosan and sodium lauryl sulfate, as well as other combinations, also significantly improved the permeability of metformin. Therefore, permeability enhancers could be used in the formulation development strategies of furosemide and metformin by the pharmaceutical industry.
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Elena Gras-Colomer, Victor Mangas-Sanjuan, Maria-Amparo Martinez-Gomez, Monica Climente-Marti, Matilde Merino-Sanjuan
Summary: A population pharmacokinetic model of enzyme activity in Gaucher-type 1 (GD1) patients after intravenous administration of enzyme replacement therapy (ERT) was developed, and an exposure-efficacy relationship for bone marrow infiltration was established. The findings of this study are of significant clinical relevance for the individualization of ERT in GD1 patients.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Evren Gundogdu, Emine-Selin Demir, Meliha Ekinci, Emre Ozgenc, Derya Ilem-Ozdemir, Zeynep Senyigit, Isabel Gonzalez-Alvarez, Marival Bermejo
Summary: Imatinib-loaded nanostructured lipid carrier system (F16-IMT) was successfully developed and characterized in this study. The F16-IMT formulation exhibited small particle size, stability, and efficient drug release. It also showed good cellular uptake and cytotoxicity against CRL-1739 cells. These findings suggest that F16-IMT has the potential to be an effective treatment for GIST.
Article
Pharmacology & Pharmacy
Claudia Miranda, Alejandro Ruiz-Picazo, Paula Pomares, Isabel Gonzalez-Alvarez, Marival Bermejo, Marta Gonzalez-Alvarez, Alex Avdeef, Miguel-Angel Cabrera-Perez
Summary: The aim of this study was to characterize the biopharmaceutical properties of a new hybrid benzodiazepine-dihydropyridine derivative, JM-20. The results showed that JM-20 had higher solubility at acidic pH but low solubility overall. It also exhibited high permeability and absorption carrier-mediated transport mechanism. Based on the biopharmaceutical classification system, JM-20 was provisionally classified as class 2.
Review
Pharmacology & Pharmacy
Karine Rodriguez-Fernandez, Victor Mangas-Sanjuan, Matilde Merino-Sanjuan, Antonio Martorell-Calatayud, Almudena Mateu-Puchades, Monica Climente-Marti, Elena Gras-Colomer
Summary: The emergence of biological therapies has revolutionized the treatment of psoriasis. However, the complex pharmacokinetic properties of monoclonal antibodies (mAb) have posed challenges for dosage determination. This study reviewed population pharmacokinetic/pharmacodynamic (PK/PD) models of different types of mAb used in psoriasis treatment. The results showed that body weight and immunogenicity significantly influenced drug clearance. The lack of consensus on PK/PD relationships emphasizes the importance of therapeutic drug monitoring (TDM) in psoriasis treatment.
Article
Chemistry, Multidisciplinary
Emre Ozgenc, Merve Karpuz, Ege Arzuk, Marta Gonzalez-Alvarez, Marival Bermejo Sanz, Evren Gundogdu, Isabel Gonzalez-Alvarez
Summary: This study prepared radiolabeled trastuzumab loaded solid lipid nanoparticles and investigated their biocompatibility, cellular uptake, and apoptosis induction capacity. The results showed that these nanoparticles exhibited favorable physicochemical properties and pharmacokinetic profiles.
Article
Pharmacology & Pharmacy
Isabel Gonzalez-alvarez, Barbara Sanchez-Dengra, Raquel Rodriguez-Galvez, Alejandro Ruiz-Picazo, Marta Gonzalez-alvarez, Alfredo Garcia-Arieta, Marival Bermejo
Summary: In the past, excipients were considered unimportant in the absorption process, but research has shown that this belief is not always true. This study investigated the reasons for a bioequivalence failure between two formulations of silodosin and found that the differences in excipients can indeed affect the drug's permeability through the gastrointestinal barrier.
Article
Pharmacology & Pharmacy
Barbara Sanchez-Dengra, Elena Garcia-Montoya, Isabel Gonzalez-Alvarez, Marival Bermejo, Marta Gonzalez-Alvarez
Summary: The aim of this study was to create and validate a new in vitro model capable of predicting the permeability across the blood-brain barrier in the presence of glioblastoma. The results showed that the co-culture model of MDCK and MDCK-MDR1 with U87-MG had high predictability in drug access to the central nervous system.
Article
Biotechnology & Applied Microbiology
Barbara Sanchez-Dengra, Maria Alfonso, Isabel Gonzalez-Alvarez, Marival Bermejo, Marta Gonzalez-Alvarez, Ramon Martinez-Manez
Summary: The two types of nanoparticles loaded with ponatinib have been proven to be non-toxic to blood-brain barrier cells and have shown improved drug delivery to the brain compared to the free drug when administered intranasally. This research is significant for the treatment of brain cancer.
Meeting Abstract
Gastroenterology & Hepatology
Christopher Broxson, Milica Markovic, Arik Dahan, Shimon Ben-Shabat, Bikash Sahay, Ellen M. Zimmermann