Article
Chemistry, Medicinal
Jie Zang, Felix Peters, Yves Cambet, Eugenia Cifuentes-Pagano, Munira Mohamed Shishay Hissabu, Christopher M. Dustin, Lars Henrik Svensson, Martin Mariboe Olesen, Mathias Feldt Lomholt Poulsen, Stig Jacobsen, Pernille Sonderby Tuelung, Dilip Narayanan, Annette Eva Langkilde, Michael Gajhede, Patrick J. Pagano, Vincent Jaquet, Frederik Vilhardt, Anders Bach
Summary: In this study, we optimized and explored bivalent small-molecule drugs targeting the NOX2 subunit p47phox and identified compounds with high binding affinities and cellular activities, demonstrating the potential of p47phox as an effective small-molecule target. This has important implications for the research and development of therapeutic drugs for related diseases.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Immunology
Yingze Ye, Zhihong Jian, Tong Jin, Yina Li, Zhi Zeng, Xu Zhang, Xiaoxing Xiong, Lijuan Gu
Summary: This study suggests that NADPH oxidase 2 (NOX2)-mediated reactive oxygen species (ROS) production exacerbates acute brain injury but promotes functional recovery after stroke. Inhibiting NOX2 activity can reduce infarct size and mortality, but may hinder functional recovery. This effect may be related to neuroinflammation, autophagy, angiogenesis, and the PI3K/Akt signaling pathway.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Pharmacology & Pharmacy
Valentin Platel, Diane Lechevalier, Clara Bourreau, Sarah Renault, Ivana Soborova, Caroline Jeanniere, Ludovic Martin, Olivier Herault, Isabelle Corre, Nicolas Clere
Summary: The tumor microenvironment is important for the progression of melanoma, and recent studies have found that endothelial cells (EC) play a role in the endothelial-to-mesenchymal transition (EndMT). Reactive oxygen species (ROS), primarily produced by NAPDH oxidases (NOX), can control tumor growth. This study aimed to investigate the role of NOX1 and NOX2 in EndMT induced by conditioned media (CM) from melanoma cells. The results showed that NOX1 and NOX2 played important roles in EndMT.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Cesar I. Ortiz-Garcia, Monica L. Salgado-Lucio, Ana L. Roa-Espitia, Aide A. Munoz-Sanchez, Joaquin Cordero-Martinez, Enrique O. Hernandez-Gonzalez
Summary: This study found that guinea pig and mouse spermatozoa express NOX2 and NOX4 during capacitation, and these enzymes play an important role in ROS production. The study also discovered an interaction between NOX2 and NOX4, which depends on the activation of calpain. The results indicate that NOX2 and NOX4 are the most important ROS producers during guinea pig and mouse sperm capacitation, and their activation depends on calpain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Hasnaa Maksouri, Dounia Darif, Jerome Estaquier, Myriam Riyad, Christophe Desterke, Meryem Lemrani, Pham My-Chan Dang, Khadija Akarid
Summary: This study evaluated the impact of Moroccan strains of L. major and L. tropica on PMNs. The results showed that L. tropica inhibited O-2(-) production, while L. major did not. Additionally, Leishmania soluble antigens from both species inhibited O-2(-) induction.
Article
Materials Science, Biomaterials
Mousumi Baruah, Anal Jana, Mudassar Ali, Koyeli Mapa, Animesh Samanta
Summary: The innate immune system eliminates pathogens through phagocytosis and the production of reactive oxygen species (ROS), such as the pro-inflammatory oxidant hypochlorous acid (HOCl). This study developed a pentacyclic pyridinium probe, PM-S, to efficiently detect HOCl in the Nox2 pathway. The probe showed advantages in terms of stability, sensitivity, and specificity to mitochondria, providing a novel platform for monitoring the inflammatory process.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Cell Biology
Daniel Simoes de Jesus, Paula Bargi-Souza, Vinicius Cruzat, Vijay Yechoor, Angelo Rafael Carpinelli, Rodrigo Antonio Peliciari-Garcia
Summary: The pancreatic beta cells circadian clock and NADPH oxidase (NOX) family play important roles in glucose metabolism. Absence of BMAL1 may trigger an increase in NOX2-derived reactive oxygen species (ROS) in pancreatic beta cells, leading to inhibition of insulin secretion.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Medicine, Research & Experimental
Hyungkuen Kim, Eunmi Hwang, Byung-Chul Park, Sung-Jo Kim
Summary: Reactive oxygen species (ROS) play a crucial role in regulating the proliferation, metastasis, and drug resistance of melanoma, and the inhibition of NOX2 and cytosolic ROS by Dudleya brittonii water extract (DBWE) and its bioactive substance polygalatenoside A may contribute to the prevention and treatment of melanoma. DBWE also inhibits mitochondrial activity, lipid metabolism, and cell cycle in B16-F10 melanoma cells.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Virology
Baisong Zheng, Xiaolei Zhou, Li Tian, Jian Wang, Wenyan Zhang
Summary: This study reveals that EV71 can antagonize the ISG OAS3, which is the major determinant of IFN-beta 1b-mediated inhibition of EV71 replication. OAS3 functions by activating downstream RNase L. EV71 infection induces the expression of OAS3 but not RNase L through the IFN pathway. Mechanistically, EV71 or IFN-beta 1b-induced phosphorylation of STAT1 initiates the transcription of OAS3 by directly binding to its promoter.
Article
Biochemistry & Molecular Biology
Hana Valenta, Sophie Dupre-Crochet, Mouna Abdesselem, Tania Bizouarn, Laura Baciou, Oliver Nusse, Ariane Deniset-Besseau, Marie Erard
Summary: The phagocyte NADPH oxidase is a key enzyme of the innate immune system, and the use of the chimeric protein Trimera can activate the enzyme and sustain high levels of activity, but it leads to intracellular acidification, apoptosis, and local peroxidation of lipids in the membrane.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Yuanyuan Cao, Zhangwei Chen, Jianguo Jia, Ao Chen, Yanhua Gao, Juying Qian, Junbo Ge
Summary: The study found that rosuvastatin mitigates coronary microembolism (CME)-induced cardiac injury by inhibiting Nox2-induced ROS overproduction and alleviating p53/Bax/Bcl-2-dependent cardiomyocyte apoptosis.
CARDIOVASCULAR TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yutian Lei, Wenyue Zhang, Meichen Gao, Hongjin Lin
Summary: This study investigated the toxic mechanism of DEHP on grass carp hepatocyte cell line and the therapeutic effect of evodiamine. DEHP exposure increased ROS level, inhibited antioxidant enzyme activities, activated MAPK signaling pathway, and promoted cell apoptosis. Evodiamine reduced ROS level and oxidative stress, inhibited MAPK signaling pathway, alleviated DEHP-induced apoptosis, and restored antioxidant gene expression.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Ophthalmology
Jianfeng Yu, Pengfei Li, Zhuang Li, Yingqi Li, Jiawei Luo, Wenru Su, Dan Liang
Summary: The study found that tofacitinib can inhibit the polarization of M1 macrophages and delay CGR by inhibiting STAT1 activation. This suggests that tofacitinib is an effective drug for treating CGR.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ferenc L. M. Szekeres, Erik Walum, Per Wikstrom, Anders Arner
Summary: A new compound has been identified to partially inhibit the NADPH oxidase enzymes Nox 2 and 4, leading to improved contractility of cardiomyocytes and enhanced recovery of heart function after ischemia-reperfusion injury.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Maria Anele Romeo, Maria Saveria Gilardini Montani, Rossella Benedetti, Luca Giambelli, Rosella D'Aprile, Aurelia Gaeta, Alberto Faggioni, Mara Cirone
Summary: The study revealed that HHV-6B infection induces up-regulation of PD-L1 expression on monocytes, leading to impaired immune response through activation of intracellular ROS, STAT1, and STAT3 pathways. These findings provide insight into potential therapeutic targets to counteract HHV-6B-induced immune dysfunction.
