Article
Biochemistry & Molecular Biology
Mariangela Lopreiato, Samuele Di Cristofano, Rossana Cocchiola, Alessia Mariano, Libera Guerrizio, Roberto Scandurra, Luciana Mosca, Domenico Raimondo, Anna Scotto D'Abusco
Summary: The study showed that NAPA can effectively inhibit the kinase activity of IKK alpha, and confirmed through computational analysis and biochemical data that the inhibition is non-competitive. The binding site of NAPA with IKK alpha is different from that of ATP or IKKtide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Yumei Cao, Su'an Tang, Xiaoyu Nie, Zuoqing Zhou, Guangfeng Ruan, Weiyu Han, Zhaohua Zhu, Changhai Ding
Summary: The study identified miR-214-3p as a key regulator in osteoarthritis, impacting ECM metabolism and cell apoptosis in cartilage by modulating the NF-kappa B signaling pathway. Targeting IKKP, decreased miR-214-3p exacerbates OA development, suggesting it as a potential novel therapeutic target for OA.
Article
Environmental Sciences
Hongli Wang, Min Zhang, Xiuduan Xu, Shaojun Hou, Zhihui Liu, Xuejiao Chen, Chongchong Zhang, Huan Xu, Lin Wu, Kun Liu, Lun Song
Summary: This study revealed that IKK alpha played a critical role in mediating UVB-induced apoptotic responses in UVB sensitive cells by regulating the activation of the p53 pathway. Targeting the IKK alpha/p53/PERP pathway could be a potential strategy to prevent skin photo-damages induced by sunlight.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Multidisciplinary Sciences
Zhiguo Zhang, Xiao Zhong, Hong Shen, Liang Sheng, Suthat Liangpunsakul, Anna S. Lok, M. Bishr Omary, Shaomeng Wang, Liangyou Rui
Summary: Upregulation of biliary NIK promotes ductular reaction, liver inflammation, and liver fibrosis. Inhibition of biliary NIK blocks these pathological alterations by modulating secretion of cholangiokines.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Hao Zhang, Binbin Pan, Wenjuan Huang, Mengqing Ma, Feng Zhang, Linglin Jiang, Cheng Qian, Xin Wan, Changchun Cao
Summary: This study identified and elucidated the role and mechanism of IKK alpha in renal fibrosis. The findings showed that IKK alpha aggravates renal fibrosis by activating Wnt/beta-catenin signaling pathway.
Article
Biochemistry & Molecular Biology
Veronica A. Garcia-Garcia, Josefa P. Alameda, Angustias Page, Antonio Merida-Garcia, Manuel Navarro, Adrian Tejero, Jesus M. Paramio, Rosa A. Garcia-Fernandez, M. Llanos Casanova
Summary: NMSC, particularly squamous cell carcinoma (SCC), is a severe health problem in the Caucasian population. The high expression of nuclear IKK alpha in cutaneous SCCs has been suggested to be associated with increased metastatic potential. This study explored the molecular mechanisms underlying the enhanced aggressiveness of nuclear IKK alpha in SCC progression. The results indicate that nuclear IKK alpha promotes EMT-related changes in tumor cells, particularly a migratory phenotype and the expression of proteins involved in cell matrix degradation and survival. Apigenin, an anti-cancer flavonoid, was found to inhibit IKK alpha expression and attenuate the pro-tumoral EMT changes induced by cytoplasmic IKK alpha.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kuo-Feng Tseng, Ping-Hsuan Tsai, Jie-Sian Wang, Fang-Yu Chen, Ming-Yi Shen
Summary: Sesamol can alleviate macrophage-mediated renal inflammation and reduce renal fibrosis, offering a potential approach to the treatment of chronic kidney disease.
Article
Cell Biology
Xiaoyan Xu, Yaqin Sun, Xufeng Cen, Bing Shan, Qingwei Zhao, Tingxue Xie, Zhe Wang, Tingjun Hou, Yu Xue, Mengmeng Zhang, Di Peng, Qiming Sun, Cong Yi, Ayaz Najafov, Hongguang Xia
Summary: The study reveals that Metformin can induce CMA through activating the TAK1-IKKα/β signaling pathway to improve the pathogenesis of neurodegenerative diseases. Additionally, Metformin can reduce amyloid-beta plaques in the brain and reverse the molecular and behavioral phenotypes of Alzheimer's disease.
Article
Immunology
Jianxin Qiu, Xiaohang Zheng, Guoping Cai, Jiajing Ye, Ting Jiang, Lihua Chen, Ze Li, Yuhang Gong, Zhenghua Hong, Haixiao Chen
Summary: This study provides evidence supporting the potential role of AZD8330 in the management of osteoarthritis by attenuating inflammation and regulating the NF-kappa B/P65 signaling pathway to alleviate chondrocyte degradation. The findings from animal experiments and in vitro cell experiments support this conclusion.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Mohd Amir F. Abdullah, Sarah M. M. McWhirter, Zucai Suo
Summary: Hepatitis C virus NS3/NS4A protease complex interacts with host proteins, causing adverse effects on cellular function and immune response. The cleavage of immune factors by NS3/NS4A helps the virus evade innate immunity. NS3/NS4A treatment reduces the kinase activities of IKK alpha and IKK beta, deactivating NF-kappa B-associated innate immune responses. However, it enhances the kinase activities of IKK epsilon and TBK1, facilitating antiviral pathways. After longer cleavage, IKK epsilon and TBK1 gradually lose their kinase activities, leading to the establishment of chronic HCV infection.
Article
Immunology
Qing Ruan, Cuijie Wang, Yunfeng Zhang, Jiayang Sun
Summary: This study found that brevilin A can protect mice against osteoarthritis by suppressing inflammatory response and ferroptosis through regulating the SIRT1/Nrf2/GPX4 signaling pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Irene Pecharroman, Laura Sole, Daniel Alvarez-Villanueva, Teresa Lobo-Jarne, Josune Alonso-Maranon, Joan Bertran, Yolanda Guillen, Angela Montoto, Maria Martinez-Iniesta, Violeta Garcia-Hernandez, Gemma Gimenez, Ramon Salazar, Cristina Santos, Marta Garrido, Eva Borras, Eduard Sabido, Ester Bonfill-Teixidor, Raffaella Iurlaro, Joan Seoane, Alberto Villanueva, Mar Iglesias, Anna Bigas, Lluis Espinosa
Summary: This research reveals a functional link between IKK-a, BRD4, and JAK/STAT signaling with clinical relevance in colorectal cancer. It also suggests that simultaneous inhibition of BRAF-dependent IKK-a activity, BRD4, and the JAK/STAT pathway enhances the therapeutic potential of chemotherapy drugs and could be curative in chemotherapy-resistant models.
Article
Cell Biology
Tingting Luo, Suzhen Yang, Tianming Zhao, Hanlong Zhu, Chunyan Chen, Xiaoxiao Shi, Di Chen, Kai Wang, Kang Jiang, Dan Xu, Ming Cheng, Juan Li, Wenting Li, Weijun Xu, Lin Zhou, Mingzuo Jiang, Bing Xu
Summary: Drug-induced liver injury (DILI) is a major cause of acute liver failure (ALF). DDX3X plays a role in pro-survival stress granule (SG) assembly, but its role in DILI is unknown. This study revealed that DDX3X expression is decreased in DILI patients and DDX3X deficient mice. DDX3X deficiency aggravated liver injury in multiple DILI models by enhancing oxidative stress, hepatocyte death, and inflammatory responses.
CELL DEATH & DISEASE
(2023)
Article
Neurosciences
Carl G. Gottschalk, Malabendu Jana, Avik Roy, Dhruv R. Patel, Kalipada Pahan
Summary: Research suggests that gemfibrozil can protect dopaminergic neurons in an animal model of PD, increase neurotransmitters in the striatum, and improve locomotor activities.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Orthopedics
B. Bodic, M. A. Boutet, C. Boyer, B. Metayer, C. Vignes, J. Lesoeur, J. Veziers, V. Daguin, F. Haspot, Y. Maugars, F. Djouad, J. Guicheux, C. Vinatier
Summary: This study demonstrated the feasibility of inducing osteoarthritis (OA) in immunologically humanized mice by destabilization of the medial meniscus (DMM). The developed OA model could serve as a useful tool for bridging the gap between preclinical and clinical evaluation of immune interacting disease-modifying osteoarthritis drugs (DMOADs) and cell-based therapies.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Article
Orthopedics
Matteo Baldassarri, Roberto Buda, Luca Perazzo, Diego Ghinelli, Ricciardello Sarino, Brunella Grigolo, Cesare Faldini
Summary: This study retrospectively analyzed the clinical and imaging results of 18 patients with OCD treated with one-step BMDCT transplantation. The results showed that the one-step technique can effectively restore the articular surface and achieve significant improvements in clinical and imaging evaluations.
EUROPEAN JOURNAL OF ORTHOPAEDIC SURGERY AND TRAUMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Francesca Veronesi, Silvia Brogini, Angela De Luca, Davide Bellini, Veronica Casagranda, Milena Fini, Gianluca Giavaresi
Summary: This study compares two collagen I (COLL)/chitosan (CS) coatings for titanium alloy implants and finds that both coatings enhance cell adhesion, viability, and bone matrix production, making them promising for use as bone implants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Fatehia Bushara, Melania Maglio, Gregorio Marchiori, Gianluca Giavaresi, Alberto Signoroni, Fabrizio Guerrini, Nicola Francesco Lopomo
Summary: In this study, a microCT system was used to acquire human hamstring tendons after specific preparations to enhance image contrast. The acquired images were then processed using dedicated techniques to highlight the fibrous structure and identify specific geometric features. This study designed and preliminarily validated a novel microCT protocol for the microstructural analysis of fibrous tissues.
JOURNAL OF MECHANICS IN MEDICINE AND BIOLOGY
(2023)
Article
Medicine, General & Internal
Alberto Ruffilli, Marco Manzetti, Francesca Barile, Marco Ialuna, Tosca Cerasoli, Giovanni Viroli, Francesca Salamanna, Deyanira Contartese, Gianluca Giavaresi, Cesare Faldini
Summary: Proximal junctional disease (PJD) and surgical site infection (SSI) are common complications after spine surgery. This study aimed to evaluate the influence of sarcopenia and osteopenia on these complications. Preoperative MRI was used to measure central sarcopenia and osteopenia. The analysis showed that comorbidity index and dural tear were independent risk factors for SSI, while age and diabetes were risk factors for PJD. However, there was no correlation between low M-score and PLVI and a higher complications rate.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Francesca Silingardi, Stefania Pagani, Alessandro Gambardella, Gianluca Giavaresi, Adriana Bigi, Elisa Boanini
Summary: Curcumin can be combined with calcium phosphates to develop materials with anti-oxidant properties and beneficial effects on bone tissue. At low concentrations, curcumin inhibits osteoclast growth while supporting the growth and colonization of osteoblasts.
Article
Biochemistry & Molecular Biology
Matteo Berni, Francesca Veronesi, Milena Fini, Gianluca Giavaresi, Gregorio Marchiori, Francesca Oliviero
Summary: This study aimed to investigate the relationship between articular tissue structure/composition and cartilage viscoelasticity in a large animal model of early osteoarthritis (OA) treated with orthobiologics. The results showed a potential correlation between cartilage elastic modulus and cartilage thickness (CT), synovial fluid interleukin 6 (IL6), and prostaglandin E2 (PGE2), as well as between cartilage relaxation time and CT and PGE2. This work provides important indications for future studies focusing on these relationships and advanced regeneration targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Francesca Veronesi, Deyanira Contartese, Laura Di Sarno, Veronica Borsari, Milena Fini, Gianluca Giavaresi
Summary: This systematic review collected in vitro studies conducted over the past decade to evaluate the molecular and cellular mechanisms of cell senescence and identify treatments capable of delaying senescence. The studies used in vitro models of senescence in musculoskeletal tissues, providing powerful tools to evaluate age-related changes and pathologies and contributing to the development of new therapeutic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Francesca Veronesi, Melania Maglio, Silvia Brogini, Antonio Mazzotti, Elena Artioli, Gianluca Giavaresi
Summary: Retrograde drilling (RD) is a minimally invasive surgical procedure used for non-displaced osteochondral lesions, showing positive results with a good rate of patient satisfaction, improvements in clinical scores, pain reduction, and return to daily activities and sports. Further studies are necessary to investigate post-surgical complications and gender-related responses.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Medicine, General & Internal
Francesca Salamanna, Silvio Caravelli, Laura Marchese, Melania Carniato, Emanuele Vocale, Giammarco Gardini, Giulia Puccetti, Massimiliano Mosca, Gianluca Giavaresi
Summary: This review systematically analyzes the changes in proprioception and related mechanoreceptors in osteoarthritis (OA) patients. The studies found that OA patients may have impaired proprioceptive accuracy in the posterior cruciate ligament (PCL), possibly due to changes in mechanoreceptors. Significant proprioceptive deficits were also identified in patients with knee articular cartilage lesions. Further research is needed to clarify the role of proprioception and related mechanoreceptors in OA.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Deyanira Contartese, Laura Di Sarno, Francesca Salamanna, Lucia Martini, Milena Fini, Gianluca Giavaresi, Francesca Veronesi
Summary: This systematic review examines the current body of research on in vivo models of musculoskeletal frailty, delving into various animal models simulating specific features of the condition. The review also explores the translational prospects of these models in enhancing our understanding and streamlining the production of therapeutic approaches. It provides valuable insights and guidance for researchers and practitioners in combating musculoskeletal frailty.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Alessio Bucciarelli, Alessandro Pedranz, Laura Gambari, Mauro Petretta, Leonardo Vivarelli, Dante Dallari, Brunella Grigolo, Devid Maniglio, Francesco Grassi
Summary: In this study, ultrasonication was used to control the size distribution of human bone particles, tuning their osteogenic potential. The ultrasonication process decreased the size of the bone particles and improved their biological response.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biochemistry & Molecular Biology
Stefania Pagani, Manuela Salerno, Giuseppe Filardo, Janis Locs, Gerjo J. V. M. van Osch, Jana Vecstaudza, Laura Dolcini, Veronica Borsari, Milena Fini, Gianluca Giavaresi, Marta Columbaro
Summary: The study aimed to evaluate the in vitro osteogenic potential of a collagen/collagen-magnesium-hydroxyapatite (Col/Col-Mg-HAp) scaffold functionalized with Bone Morphogenetic Protein-2 (BMP-2) and strontium-ion-enriched amorphous calcium phosphate (Sr-ACP). The results showed that the addition of BMP-2 improved the morphology and activity of osteoblasts on the scaffold, leading to increased expression of genes related to osteoblast differentiation and matrix synthesis. This suggests that the incorporation of BMP-2 enhances the efficacy of the osteochondral scaffold in challenging conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Cell & Tissue Engineering
Alessandro Gambardella, Marco Bontempi, Alessia Romanelli, Milena Fini, Gianluca Giavaresi
TISSUE ENGINEERING PART A
(2023)
Meeting Abstract
Cell & Tissue Engineering
Melania Maglio, Gregorio Marchiori, Alberto Grassi, Giacomo Dal Fabbro, Matteo Berni, Massimiliano Baleani, Stefano Zaffagnini, Gianluca Giavaresi
TISSUE ENGINEERING PART A
(2023)