Article
Optics
Shane Carney, Ting Chean Khoo, Alireza Sheikhsofla, Samaneh Ghazanfarpour, Anna Sharikova, Supriya D. Mahajan, Alexander Khmaladze, Jonathan C. Petruccelli
Summary: Quantitative phase imaging (QPI) allows label-free assessment of live cells, and digital holographic microscopy (DHM) is an accurate and reliable technique for QPI. However, the assembly and operation of DHM can be challenging for many labs. The transport of intensity equation (TIE) imaging is a QPI method that is compatible with conventional microscopy and has lower cost and coherence requirements compared to DHM. Here, a microscope capable of performing simultaneous DHM and TIE phase reconstructions is presented to validate TIE as an accurate method for both static sample arrays and live cells.
OPTICS AND LASERS IN ENGINEERING
(2023)
Article
Optics
Simcha K. Mirsky, Natan T. Shaked
Summary: Six-pack holography is used to reject out-of-focus objects in dynamic samples by illuminating the sample from six different angles and pixel-wise averaging the reconstructed images. This significantly reduces the contribution of out-of-focus objects by up to 83%, improving system sectioning capabilities.
Article
Optics
Maria J. Lopera, Carlos Trujillo
Summary: This article presents a holographic point source (HPS) developed for digital lensless holographic microscopy (HPS-DLHM). The HPS is an off-axis phase transmission hologram recorded on a photopolymer holographic film. The HPS-DLHM technique offers superior mechanical stability, wider illumination cones, and shorter reconstruction distances compared to conventional pinhole-based DLHM. This technique shows potential for applications in biomedical and telemedicine fields.
Article
Physics, Multidisciplinary
Jianglei Di, Ji Wu, Kaiqiang Wang, Ju Tang, Ying Li, Jianlin Zhao
Summary: Digital holographic microscopy combined with deep learning technology enables quantitative measurement of transparent specimens and investigation of biological samples, and has been applied in biological research. This paper proposes a neural network PhaseNet for the reconstruction of digital holograms and implements a deep learning-based holographic microscope, successfully measuring living mouse osteoblastic cells.
FRONTIERS IN PHYSICS
(2021)
Article
Biochemical Research Methods
Xiaomin Lai, Sheng Xiao, Chen Xu, Shanhui Fan, Kaihua Wei
Summary: Digital holographic microscopy is commonly used to obtain quantitative phase information of transparent cells, but the presence of aberrations can affect the accuracy of the results. A technique utilizing derivative-based principal component analysis (dPCA) has been proposed to achieve aberration-free phase imaging efficiently and effectively by extracting and compensating for almost all aberrations.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Chemistry, Analytical
Wei Yan, Yangrui Huang, Luwei Wang, Jin Li, Yong Guo, Zhigang Yang, Junle Qu
Summary: This study demonstrates a multi-color two-photon imaging method with single-wavelength excitation. The problem of spectral crosstalk is effectively solved by selecting suitable filter combinations and applying image subtraction. The results show that two-color and three-color two-photon imaging can be achieved using a single femtosecond laser.
Article
Optics
Naru Yoneda, Yusuke Saita, Takanori Nomura
Summary: Common-path off-axis single-pixel holographic imaging (COSHI) is proposed to obtain complex amplitude information using an in-line interferometer and a single-pixel detector. COSHI is robust to disturbances and requires fewer measurements compared to conventional single-pixel digital holography. The method is theoretically confirmed and numerical and experimental results demonstrate its feasibility.
Article
Optics
Sanduni I. Fernando, Jason T. Martineau, Robert J. Hobson, Thien N. Vu, Brian Baker, Brian D. Mueller, Rajesh Menon, Erik M. Jorgensen, Jordan M. Gerton
Summary: By engineering the point-spread function (PSF) of single molecules, different fluorophore species can be imaged simultaneously and distinguished by their unique PSF patterns. In this study, we inserted a silicon-dioxide phase plate at the Fourier plane of the detection path of a wide-field fluorescence microscope to generate distinguishable PSFs (X-PSFs) at different wavelengths. We demonstrated that the resulting PSFs can be localized spatially and spectrally using a maximum-likelihood estimation algorithm and can be used for hyper-spectral super-resolution microscopy of biological samples.
Article
Chemistry, Multidisciplinary
Yi Luo, Yichen Wu, Liqiao Li, Yuening Guo, Ege Cetintas, Yifang Zhu, Aydogan Ozcan
Summary: This study utilized computational microscopy and deep learning to measure the volatility of aerosols generated by electronic cigarettes. The findings suggest that the composition of e-liquids, including nicotine and flavor additives, can significantly alter the volatility of e-cig aerosol particles.
Article
Physics, Applied
Junsheng Lu, Yanan Zeng, Xinyu Chang, Yujian Hong, Xiaodong Hu
Summary: This study utilizes an infrared digital holographic method to test the morphology of biological samples in turbid media, achieving real-time three-dimensional morphology measurement and proving the correctness of phase reconstruction in the infrared system with a deviation of less than 10% compared to visible-light systems.
APPLIED PHYSICS EXPRESS
(2021)
Article
Chemistry, Physical
Yue Gou, Hui Feng Ma, Shi Sun, Tai Yi Zhang, Liang Wei Wu, Zheng Xing Wang, Sen Zheng, Tie Jun Cui
Summary: Simultaneous realization of printing and holographic images is an emerging technology that improves optical storage and anti-counterfeiting. This paper proposes a four-channel metasurface that can support four independent circular polarization information channels. The metasurface allows for flexible design of operation frequencies and achieves polarization multiplexing and frequency multiplexing. Experimental results show that printing and holographic images with different handedness can be stored at one or two different frequencies. This metasurface provides a new avenue for the design of photonic devices with multiple functionalities, with potential applications in anti-counterfeiting, data storage, and image display.
Article
Optics
Jingwen Li, Changqian Zhuang, Xi Chen, Yi Xiong, Amin Engamevis
Summary: In this study, an on-chip microscopy holography platform with an inertial impactor was presented for particle metrology and classification. The particles were first sampled and classified based on their inertia, and then their location and size were detected using digital in-line holography imaging techniques. For the first time, particles with different inertia were classified and their mass densities were estimated. An iOS-based app interface was developed for remote control of the imaging process. The proposed platform offers advantages such as compact size, efficient reconstruction algorithm, and remote operation, making it particularly relevant for industrial applications.
OPTICS AND LASER TECHNOLOGY
(2022)
Article
Optics
Yufei Dou, Miao Cao, Xiaodong Wang, Xing Liu, Xin Yuan
Summary: We introduce a coded aperture temporal compressive digital holographic microscopy (CATCHY) system that combines snapshot compressive imaging (SCI) with digital holographic microscopy to capture high-speed high-resolution samples. The system utilizes a two-dimensional detector to sample spatiotemporal data in a compressive manner, and a deep learning-based video SCI reconstruction algorithm to reconstruct the desired data cube. Experimental results show the potential of the CATCHY system in visualizing the 3D dynamic process of micro-nanostructures and imaging biological samples with high spatial and temporal resolution.
Article
Optics
Alexander Groeger, Giancarlo Pedrini, Daniel Claus, Igor Alekseenko, Felix Gloeckler, Stephan Reichelt
Summary: In this paper, the authors demonstrate digital holographic imaging through a 27-m-long fog tube filled with ultrasonically generated fog, showing its potential for imaging through scattering media. The results indicate that holographic imaging requires significantly less illumination power compared to conventional imaging for the same imaging range. They also provide a simulation model and quantitative analysis on the influence of various physical parameters on the imaging range.
Article
Chemistry, Analytical
Kaiqi Lang, Hui Cai, Xiaoping Wang
Summary: Detecting marine plankton using digital holographic microscopy (DHM) has been successfully implemented. This study proposes a new image fusion method called DHM-FCN, based on improved FCN, which can effectively display plankton images in seawater. Additionally, a neural network model, YOLOv4, was established for accurate plankton identification and localization, achieving high precision.
Article
Chemistry, Multidisciplinary
Dhruv P. Singh, William E. Uspal, Mihail N. Popescu, Laurence G. Wilson, Peer Fischer
ADVANCED FUNCTIONAL MATERIALS
(2018)
Article
Multidisciplinary Sciences
Marco J. Kuehn, Felix K. Schmidt, Nicola E. Farthing, Florian M. Rossmann, Bina Helm, Laurence G. Wilson, Bruno Eckhardt, Kai M. Thormann
NATURE COMMUNICATIONS
(2018)
Article
Biochemical Research Methods
Luis M. de Pablos, Tiago R. Ferreira, Adam A. Dowle, Sarah Forrester, Ewan Parry, Katherine Newling, Pegine B. Walrad
MOLECULAR & CELLULAR PROTEOMICS
(2019)
Article
Parasitology
Eliza Vanessa Carneiro Alves-Ferreira, Tiago Rodrigues Ferreira, Pegine Walrad, Paul M. Kaye, Angela Kaysel Cruz
PARASITES & VECTORS
(2020)
Article
Biochemistry & Molecular Biology
Tiago R. Ferreira, Adam A. Dowle, Ewan Parry, Eliza V. C. Alves-Ferreira, Karen Hogg, Foteini Kolokousi, Tony R. Larson, Michael J. Plevin, Angela K. Cruz, Pegine B. Walrad
NUCLEIC ACIDS RESEARCH
(2020)
Article
Multidisciplinary Sciences
Katie L. Thornton, Jaimi K. Butler, Seth J. Davis, Bonnie K. Baxter, Laurence G. Wilson
NATURE COMMUNICATIONS
(2020)
Article
Infectious Diseases
Samuel Carmichael, Ben Powell, Thomas Hoare, Pegine B. Walrad, Jonathan W. Pitchford
Summary: Leishmaniasis, a neglected tropical disease, causes an estimated 50,000 deaths annually in lower to middle income countries. Recent research highlights the importance of skin heterogeneity and parasite population dynamics within sand fly vectors in disease transmission. Shortening the lifespan of sand flies appears to be an effective strategy in reducing Leishmania transmission.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Multidisciplinary Sciences
N. Baker, C. M. C. Catta-Preta, R. Neish, J. Sadlova, B. Powell, E. V. C. Alves-Ferreira, V. Geoghegan, J. B. T. Carnielli, K. Newling, C. Hughes, B. Vojtkova, J. Anand, A. Mihut, P. B. Walrad, L. G. Wilson, J. W. Pitchford, P. Volf, J. C. Mottram
Summary: This study identified important protein kinase genes in Leishmania mexicana that are involved in transition between different life cycle stages, as well as revealed their functions in parasitism in macrophages, colonization of the sand fly, and motility.
NATURE COMMUNICATIONS
(2021)
Article
Infectious Diseases
Juliana Alcoforado Diniz, Mariana M. Chaves, Slavica Vaselek, Rubens D. Miserani Magalhaes, Rafael Ricci-Azevedo, Renan V. H. de Carvalho, Lucas B. Lorenzon, Tiago R. Ferreira, Dario Zamboni, Pegine B. Walrad, Petr Volf, David L. Sacks, Angela K. Cruz
Summary: The study investigates the post-transcriptional gene expression control mechanisms in Leishmania, revealing the impact of PRMT7 on parasite development and pathogenicity. Higher levels of PRMT7 can impair parasite pathogenicity, and this study highlights how post-transcriptional regulation can affect different aspects of the parasite biology.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Review
Parasitology
Suellen Rodrigues Maran, Krista Fleck, Natalia Melquie Monteiro-Teles, Tony Isebe, Pegine Walrad, Victoria Jeffers, Igor Cestari, Elton J. R. Vasconcelos, Nilmar Moretti
Summary: Protein lysine acetylation is a major regulatory post-translational modification across different organisms, affecting chromatin structure, gene expression, and various cellular processes. The discovery of acetylomes in protozoan parasites suggests that acetylation may regulate crucial biological processes in these parasites. Furthermore, the regulation of glycolytic enzymes by acetylation in protozoans indicates the potential role of this modification in regulating other processes essential for parasite survival and adaptation during their life cycle.
TRENDS IN PARASITOLOGY
(2021)
Editorial Material
Parasitology
Pegine B. Walrad, Mark. C. Field, Miguel Navarro, Derrick R. Robinson
Summary: The British Society for Parasitology (BSP) holds a biannual symposium devoted to kinetoplastids, covering a wide range of research into these organisms and alternating with the Woods Hole Kinetoplastid Molecular Cell Biology meeting. The 2020 symposium in Granada, Spain was overshadowed by news of the emerging novel coronavirus, but still managed to showcase excellent science in a spectacular setting. The editorial aims to capture some of the excellent science presented at the meeting and provide attendees with fond memories through images and highlights from the event.
Review
Biochemistry & Molecular Biology
Gustavo D. Campagnaro, Edward Nay, Michael J. Plevin, Angela K. Cruz, Pegine B. Walrad
Summary: A large number of eukaryotic proteins are regulated by various post-translational covalent modifications, with arginine methylation playing a significant role in affecting protein interactions. Kinetoplastids, with their unique genetic features, serve as excellent models for studying post-transcriptional regulation of gene expression. The roles of PRMTs in controlling RBPs in pathogenic kinetoplastids have been extensively studied, with important discoveries made in recent years.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Neurosciences
Amy C. Cording, Nicolas Shiaelis, Stavroula Petridi, C. Adam Middleton, Laurence G. Wilson, Christopher J. H. Elliott
NPJ PARKINSONS DISEASE
(2017)
