Article
Medicine, Research & Experimental
Yuto Kubo, Koji Tanaka, Yasunori Masuike, Tsuyoshi Takahashi, Kotaro Yamashita, Tomoki Makino, Takuro Saito, Kazuyoshi Yamamoto, Tomoyuki Tsujimoto, Takashi Harino, Yukinori Kurokawa, Makoto Yamasaki, Kiyokazu Nakajima, Hidetoshi Eguchi, Yuichiro Doki
Summary: This study revealed that decreased mtDNA copy number induced EMT through modulation of MMP and DNA methylation in ESCC. Therapeutic strategies aimed at increasing mtDNA copy number and using DNMT inhibitors may be effective in preventing EMT and chemosensitivity resistance.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Rahul Advani, Sara Luzzi, Emma Scott, Caroline Dalgliesh, Joachim Weischenfeldt, Jennifer Munkley, David J. Elliott
Summary: This article discusses the expression of splicing regulators ESRP1 and ESRP2 in aggressively proliferating primary prostate tumors as markers of disease progression. It suggests that ESRP1 and ESRP2 act as lineage survival oncogenes in prostate cancer and highlights the need to identify the gene expression targets controlled by these regulators to develop targeted therapies.
Article
Oncology
Iori Motoo, Sohachi Nanjo, Takayuki Ando, Satoshi Yamashita, Toshikazu Ushijima, Ichiro Yasuda
Summary: The study reveals that in diffuse-type gastric cancers, ULK2 is methylated and knockdown of ULK2 can induce autophagy, cell migration, and EMT. mRNA microarray analysis showed that knockdown of ULK2 altered the expressions of oncogenic genes associated with cell migration and EMT.
Review
Oncology
Samantha Gogola, Michael Rejzer, Hisham F. Bahmad, Wassim Abou-Kheir, Yumna Omarzai, Robert Poppiti
Summary: Prostate cancer is the second most common cancer in men worldwide, and treatment options include radiation therapy, surgery, and active surveillance. However, in most cases, the cancer eventually becomes castration-resistant. The transition from androgen-dependent to androgen-independent state is not well understood, but epithelial-to-non-epithelial transition plays a crucial role. This review summarizes the factors and pathways involved in this transition, as well as the diagnostic and prognostic biomarkers.
Article
Oncology
Kunning Zhang, Zhiwei Zhai, Sanshui Yu, Yu Tao
Summary: The study revealed that DNA hypermethylation-induced downregulation of ANGPTL4 promoted the activation of cancer-associated fibroblasts and epithelial-mesenchymal transition (EMT) of CRC cells through the ERK signaling pathway, thereby increasing invasion and metastasis in CRC.
Article
Cell Biology
Huafu Li, Chunming Wang, Linxiang Lan, Axel Behrens, Mona Tomaschko, Josue Ruiz, Qiao Su, Guangying Zhao, Cheng Yuan, Xing Xiao, Bo Li, Leping Yan, Wang Wu, Wuguo Li, Junzong Chen, Yulong He, Changhua Zhang
Summary: EMT plays a crucial role in the aggressiveness and metastasis of gastric cancer, especially in the infiltration of NK cells into tumor cells. Vinculin expression is closely related to cancer aggressiveness and distant metastasis, affecting patient survival prognosis. Additionally, VCL can influence EMT and tumor immunity by regulating EPCAM gene expression.
Article
Cell Biology
Yuan-jie Liu, Shu-hong Zeng, Yi-dou Hu, Yong-hua Zhang, Jie-pin Li
Summary: This study demonstrates that overexpression of NREP in gastric cancer is associated with poor prognosis, potentially influencing the activation of cancer-associated fibroblasts and epithelial-mesenchymal transition. Additionally, the expression of NREP correlates with the abundance of M2 macrophages, suggesting it could serve as a prognostic biomarker and therapeutic target for gastric cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Mugdha Pol, Hanyuan Gao, He Zhang, Olivia J. George, Joseph M. Fox, Xinqiao Jia
Summary: Synthetic matrices with dynamic presentation of cell guidance cues are engineered to mimic prostate cancer progression and metastasis. A tunable hyaluronic acid-based hydrogel platform is developed using bioorthogonal tetrazine ligation with strained alkenes. The platform allows the spontaneous formation of multicellular tumoroids and the development of cellular protrusions in response to cell adhesive peptide modification. The engineered tumor model can be utilized to identify potential molecular targets and test pharmacological inhibitors, accelerating the design of innovative strategies for cancer therapeutics.
Article
Oncology
Hao Wu, Juanjuan Qiu, Zhenru Wu, Tao He, Chen Zhou, Qing Lv
Summary: This study reveals that ADCY6 is expressed at low levels in breast cancer, leading to increased proliferation, invasion, and migration of breast cancer cells. The low expression of ADCY6 is attributed to the low demethylation of TET1, and the methylation of ADCY6 can be altered by TET1. Bioinformatics analysis shows that TET1 is regulated by miR-27a-3p and regulates the methylation of ADCY6, thereby affecting the EMT process of breast cancer cells and promoting the malignant biological behavior of breast cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Minli Huang, Mengru Fu, Jia Wang, Chunhua Xia, Hong Zhang, Yuqing Xiong, Jiake He, Jianming Liu, Bingchen Liu, Siyi Pan, Fanglan Liu
Summary: Cancer-associated fibroblasts (CAFs) play a crucial role in breast cancer initiation, metastasis, and invasion. This study investigated the requirement of autophagy and FAP-alpha in breast cancer cell invasion and metastasis, finding that both are necessary. Targeting autophagy or FAP-alpha individually may be a potential approach to improve the prognosis of human breast cancer.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Enrique A. Castellon, Sebastian Indo, Hector R. Contreras
Summary: This article explores the presence and role of cancer stem cells (CSCs) and the Epithelial-Mesenchymal transition (EMT) in prostate cancer (PCa), and finds that the stemness-mesenchymal axis is a critical process related to relapse, metastasis, and resistance. Manipulating the stemness-EMT axis genes on the androgen receptor (AR) may shed light on the effect of this axis on metastasis and castration resistance in PCa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jia Chen, Shu-Fen Hou, Feng-Jie Tang, Dai-Song Liu, Zi-Zi Chen, Hong-Lian Zhang, Shao-Hua Wang
Summary: HOTAIR, through up-regulating Sp1 and targeting miR-199a, promotes stemness and progression of CSCC. Targeting HOTAIR, Sp1 or the underlying mechanisms may benefit CSCC treatment, as shown by functional assays and in vivo models.
Article
Biotechnology & Applied Microbiology
Guang-Cheng Luo, Lin Chen, Jiang Fang, Zhi-Jian Yan
Summary: This study revealed that hsa_circ_0030586 is significantly upregulated in prostate cancer cells and may promote epithelial-mesenchymal transition (EMT) through the PI3K-AKT signaling pathway. Experiment results showed that interfering with hsa_circ_0030586 could inhibit tumor cell proliferation, migration, and invasion in PCa.
Article
Biochemistry & Molecular Biology
Janae D. Sweeney, Marija Debeljak, Stacy Riel, Ana Cecilia Millena, James R. Eshleman, Channing J. Paller, Valerie Odero-Marah
Summary: The research showed that SOD2 expression did not correlate with tumor aggressiveness or SOD2 genotype in prostate cancer. However, the Ala-SOD2 allele may promote EMT, leading to increased cell migration. The antioxidant MSKE could inhibit EMT mediated by Ala-SOD2 SNP, indicating promising therapeutic potential for halting prostate cancer progression.
Article
Medicine, Research & Experimental
Yao Li, Quan Li, Dujian Li, Jie Gu, Duocheng Qian, Xiaojing Qin, Yu Chen
Summary: The study found that exosomal PSGR promoted migration, invasion, stemness, and epithelial-mesenchymal transitions of prostate cancer cells, and reshaped the mRNA profiles of the cells.
Article
Biochemistry & Molecular Biology
Fabiola Marino, Nadia Salerno, Mariangela Scalise, Luca Salerno, Annalaura Torella, Claudia Molinaro, Antonio Chiefalo, Andrea Filardo, Chiara Siracusa, Giuseppe Panuccio, Carlo Ferravante, Giorgio Giurato, Francesca Rizzo, Michele Torella, Maria Donniacuo, Antonella De Angelis, Giuseppe Viglietto, Konrad Urbanek, Alessandro Weisz, Daniele Torella, Eleonora Cianflone
Summary: Diabetic cardiomyopathy (DCM) is a major cause of cardiovascular complications in diabetes mellitus (DM). Two different types of diabetes, type 1 and type 2, exhibit different effects on cardiac structure, function and gene expression. Animal models induced by different glucose regulators have been used to compare and study these effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pathology
Nora Sahnane, Daniela Rivera, Laura Libera, Ileana Carnevali, Barbara Banelli, Sofia Facchi, Viviana Gismondi, Michele Paudice, Gabriella Cirmena, Valerio G. Vellone, Fausto Sessa, Liliana Varesco, Maria G. Tibiletti
Summary: This study found that BRCA1 promoter methylation might be a better determinant of therapy response and developed a simple and convenient assay for measurement. It has potential to be applied in clinical practice.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2023)
Article
Multidisciplinary Sciences
Sofia Pavlou, Stefanie Foskolou, Nikolaos Patikas, Sarah F. Field, Evangelia K. Papachristou, Clive D'Santos, Abigail R. Edwards, Kamal Kishore, Rizwan Ansari, Sandeep S. Rajan, Hugo J. R. Fernandes, Emmanouil Metzakopian
Summary: The accumulation of aggregated and misfolded proteins in neurodegenerative disorders can lead to endoplasmic reticulum stress. In this study, a genetic screen was performed using a human druggable genome library in human cortical neurons derived from induced pluripotent stem cells. Thirteen genes were identified and validated as neuroprotective against Tunicamycin-induced endoplasmic reticulum stress. Pharmacological inhibition of KAT2B, a lysine acetyltransferase, attenuated neuronal cell death and activation of CHOP, a key pro-apoptotic protein. Follow-up analysis suggested that the inhibition of KAT2B reversed the transcriptional changes caused by Tunicamycin. Furthermore, treatment with the KAT2B inhibitor, L-Moses, reduced total protein levels affected by Tunicamycin without altering their acetylation profile. These findings highlight KAT2B and L-Moses as potential therapeutic targets for neurodegenerative diseases.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Annamaria Salvati, Carlo Ferravante, Jessica Lamberti, Teresa Rocco, Elena Alexandrova, Ylenia D'Agostino, Maksim Sorokin, Victor Efimov, Anton Buzdin, Oriana Strianese, Giovanni Nassa, Roberta Tarallo, Alessandro Weisz, Francesca Rizzo, Giorgio Giurato
Summary: The ongoing COVID-19 pandemic has had significant implications for public health and alters the host transcriptome. To understand the virus's effect on host cell transcriptome, a dataset was generated from nasopharyngeal swabs of 35 individuals infected with SARS-CoV-2 from the Campania region in Italy. This dataset can help elucidate gene interactions and contribute to the development of therapeutic pathways.
Article
Microbiology
Christopher Riccardi, Marzia Calvanese, Veronica Ghini, Tania Alonso-Vasquez, Elena Perrin, Paola Turano, Giorgio Giurato, Alessandro Weisz, Ermenegilda Parrilli, Maria Luisa Tutino, Marco Fondi
Summary: This study investigates the maintenance of metabolic homeostasis in a cold-adapted marine bacterium during growth at significantly different temperatures. The research shows a strong metabolic stability at the level of the main central metabolites but significant changes in gene expression at the transcriptional level. These findings suggest a transcriptomic buffering of cellular metabolism to produce overlapping metabolic phenotypes despite the temperature gap.
Article
Medicine, General & Internal
Andrea Cardia, Samantha Epistolio, Ismail Zaed, Nora Sahnane, Roberta Cerutti, Debora Cipriani, Jessica Barizzi, Paolo Spina, Federico Mattia Stefanini, Michele Cerati, Sergio Balbi, Luca Mazzucchelli, Fausto Sessa, Gianfranco Angelo Pesce, Michael Reinert, Milo Frattini, Francesco Marchi
Summary: The study evaluated MGMT expression, methylation, and miRNA expression in 112 GBM patients and correlated them with clinical outcomes. The results showed significant associations between MGMT IHC, miRNA expression, and patient survival. The data reinforce the clinical relevance of miRNA expression as a marker to predict the efficacy of chemoradiation in GBM.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Marzia Di Donato, Erika Di Zazzo, Annamaria Salvati, Carmela Sorrentino, Giorgio Giurato, Donatella Fiore, Maria Chiara Proto, Monica Rienzo, Amelia Casamassimi, Patrizia Gazzerro, Maurizio Bifulco, Gabriella Castoria, Alessandro Weisz, Giovanni Nassa, Ciro Abbondanza
Summary: This study provides insights into the potential tumor-promoting functions of RIZ2 in CRC, demonstrating its involvement in EGF pathway deregulation and its impact on CRC cell behavior.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Review
Cell Biology
Elisabetta Fratta, Giorgio Giurato, Roberto Guerrieri, Francesca Colizzi, Jessica Dal Col, Alessandro Weisz, Agostino Steffan, Barbara Montico
Summary: Autophagy plays a crucial role in cutaneous melanoma, but its impact on tumor development remains controversial. Of particular interest, autophagy is increased in BRAF-mutant melanoma and impairs targeted therapy. Moreover, recent studies have highlighted the involvement of mitophagy and secretory autophagy in BRAF-mutant melanoma.
CELL DEATH DISCOVERY
(2023)
Review
Biology
Emanuele Murgo, Tommaso Colangelo, Maria Marina Bellet, Francesco Malatesta, Gianluigi Mazzoccoli
Summary: NPAS2 is a key transcription factor involved in regulating circadian rhythms and sleep-wake cycles in mammals. It plays a crucial role in metabolism regulation and maintaining internal clocks synchronized with the day-night cycle. Dysregulation of NPAS2 may lead to sleep disturbances, psychiatric disorders, and other health issues. Additionally, NPAS2 may serve as a predictive biomarker and potential therapeutic target.
Article
Cell Biology
Giorgio Giurato, Ilaria Terenzi, Francesco Chiuso, Annamaria Salvati, Francesca Rizzo, Roberta Tarallo, Alessandro Weisz, Giovanni Nassa
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)