期刊
NUCLEIC ACIDS RESEARCH
卷 45, 期 9, 页码 5470-5486出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkx094
关键词
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资金
- Natural Sciences and Engineering Research Council of Canada
- Canadian Institutes of Health Research (CIHR Grant) [11281]
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Schulich School of Medicine and Dentistry
- Ontario Graduate Scholarship
- Alexander Graham Bell Canada Graduate Scholarship (NSERC CGS-D)
- NSERC Discovery Grant
Bacterial sRNAs play an important role in regulating many cellular processes including metabolism, outer membrane homeostasis and virulence. Although sRNAs were initially found in intergenic regions, there is emerging evidence that protein coding regions of the genome are a rich reservoir of sRNAs. Here we report that the 5'UTR of IS200 transposase mRNA (tnpA) is processed to produce regulatory RNAs that affect expression of over 70 genes in Salmonella Typhimurium. We provide evidence that the tnpA derived sRNA base-pairs with invF mRNA to repress expression. As InvF is a transcriptional activator of SPI-1 encoded and other effector proteins, tnpA indirectly represses these genes. We show that deletion of IS200 elements in S. Typhimurium increases invasion in vitro and reduces growth rate, while over-expression of tnpA suppresses invasion. Our work indicates that tnpA acts as an sRNA 'sponge' that sets a threshold for activation of Salmonella pathogenicity island (SPI)-1 effector proteins and identifies a new class of 'passenger gene' for bacterial transposons, providing the first example of a bacterial transposon producing a regulatory RNA that controls host gene expression.
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