期刊
NUCLEIC ACIDS RESEARCH
卷 45, 期 13, 页码 7774-7785出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkx450
关键词
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资金
- Spanish Ministry of Economy and Competitiveness [BIO2 013-49148-C2-2-R, BIO2015-69085-REDC]
- AEI/FED ER, UE [BIO2016-76412-C2-2-R]
- Sara Borrell contract [CD13/00304]
- AEI/FEDER, UE [BIO2016-76412-C2-2-R]
Horizontal gene transfer is a key process in the evolution of bacteria and also represents a source of genetic variation in eukaryotes. Among elements participating in gene transfer, thousands of small (<10 kb) mobile bacterial plasmids that replicate by the rolling circle mechanism represent a driving force in the spread of antibiotic resistances. In general, these plasmids are built as genetic modules that encode a replicase, an antibiotic-resistance determinant, and a relaxase that participates in their conjugative mobilization. Further, they control their relatively high copy number (similar to 30 copies per genome equivalent) by antisense RNAs alone or combined with a repressor protein. We report here that the MobM conjugative relaxase encoded by the promiscuous plasmid pMV158 participates in regulation of the plasmid copy number by transcriptional repression of the antisense RNA, thus increasing the number of plasmid molecules ready to be horizontally transferred (mobilization) and/or vertically inherited (replication). This type of crosstalk between genetic modules involved in vertical and horizontal gene flow has not been reported before.
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