The MicroRNAs in the Pathogenesis of Metabolic Memory

Metabolic memory is identified as a phenomenon that previous exposure to hyperglycemia results in the long-lasting deleterious effects on cardiovascular events. More and more researches show that epigenetics plays an important role in the pathogenesis of metabolic memory. It remains unclear whether microRNA (miRNA) dysfunctions participate in the event. In this study, the miRNA arrays on human aortic endothelial cells were adopted to seek the miRNAs that may be involved in the metabolic memory and were verified in vivo and in vitro. Sixteen miRNAs were found differentially expressed. Among these miRNAs, the expressions of miR-125b, miR-146a-5p, and miR-29a-3p were associated with persistent impaired endothelial function and altered proinflammatory gene expressions, including nuclear factor-kappa B (NF-kappa B) subunit p65. Direct inhibition of miR-125b expression or increased miR-146a-5p expression blunted NF-kappa B signals and improved the endothelial function. Luciferase reporter assays confirmed the biochemical relationship for miR125b targeting on TNF-alpha-induced protein 3 and miR-146a-5p targeting on TNF receptor-associated factor 6 and IL-1 receptor-associated kinase 1 during the activation of NF-kappa B pathway. Thus, our findings demonstrate that glucose induced changes in miR-125b and miR-146a-5p are related to the long-lasting activation of NF-kappa B pathway and contribute to follow-up metabolic memory.