Article
Multidisciplinary Sciences
Anne Fabricant, Geoffrey Z. Iwata, Soenke Scherzer, Lykourgos Bougas, Katharina Rolfs, Anna Jodko-Wladzinska, Jens Voigt, Rainer Hedrich, Dmitry Budker
Summary: Upon stimulation, plants can produce electrical signals analogous to the animal nervous system, and these signals can generate measurable magnetic fields in multicellular plant systems. Researchers have recorded biomagnetism associated with electrical activity in the carnivorous Venus flytrap, paving the way for understanding the molecular basis of biomagnetism in living plants.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Carl Procko, Wen Mai Wong, Janki Patel, Seyed Ali Reza Mousavi, Tsegaye Dabi, Marc Duque, Lisa Baird, Sreekanth H. Chalasani, Joanne Chory
Summary: The evolution of the Venus flytrap's ability to sense, capture, and digest animal prey has long been a mystery in the scientific community. Recent genome and transcriptome sequencing studies have provided some insight into the genes involved in these tasks, but proving a direct link has been challenging. In this study, researchers used CRISPR-Cas9 methods to modify the Venus flytrap genome and found that mutations in two mechanosensitive ion channel genes led to a reduced response in leaf-closing behavior. The findings suggest that multiple redundant ion channels play a role in the plant's sensory system for prey detection.
Review
Plant Sciences
Rainer Hedrich, Ines Kreuzer
Summary: All plants have electrical excitability, but only few can produce a well-defined action potential (AP). The Venus flytrap Dionaea muscipula is able to generate APs with a high firing frequency and speed, allowing it to catch small animals quickly. The captured prey triggers a certain number of APs, which influences the trapping decisions made by the flytrap. The typical Dionaea AP lasts 1 second and consists of five phases, each controlled by specific ion channels, pumps, and carriers.
Article
Plant Sciences
Matthias Freund, Dorothea Graus, Andreas Fleischmann, Kadeem J. Gilbert, Qianshi Lin, Tanya Renner, Christian Stigloher, Victor Albert, Rainer Hedrich, Kenji Fukushima
Summary: To survive in nutrient-poor environments, carnivorous plants have developed traps analogous to animal digestive systems to capture and digest small organisms. These traps modify plant-specific organs, such as leaves, to acquire the ability to break down and absorb nutrients. Digestive glands in carnivorous plants secrete enzymes and other substances to digest prey, while absorbing the released compounds for nutrient uptake.
Article
Biochemistry & Molecular Biology
Sonke Scherzer, Jennifer Bohm, Shouguang Huang, Anda L. Iosip, Ines Kreuzer, Dirk Becker, Manfred Heckmann, Khaled A. S. Al-Rasheid, Ingo Dreyer, Rainer Hedrich
Summary: Since the 19th century, it has been known that the carnivorous Venus flytrap is electrically excitable, but the mechanism and molecular entities of its action potential (AP) remain unknown. This study found that the trap expressed a specific set of ion transporters during its electrically excitable stage, particularly the increase in glutamate receptor GLR3.6 RNA. Electrical stimulation or glutamate application induced an AP and cytoplasmic Ca2+ transient that propagated at the same speed along the trap's surface. The counting of APs and long-lasting Ca2+ transients directs different steps in the plant's hunting cycle.
Article
Plant Sciences
Andrej Pavlovic, Ondrej Vrobel, Petr Tarkowski
Summary: In his book, Charles Darwin observed that the response of sundew plants to stimuli is not triggered by water drops but by dissolved chemicals or mechanical stimulation. This study used modern methods to investigate this 150-year-old mystery. The findings showed that sundew plants have a weak response to water drops but significant changes in enzyme activity and digestive enzyme abundance when exposed to other stimuli.
Article
Biochemistry & Molecular Biology
Zalan Czekus, Andras Kukri, Kamiran Aron Hamow, Gabriella Szalai, Irma Tari, Attila Ordog, Peter Poor
Summary: Defense responses in tomato plants against pathogens are influenced by factors such as daytime and ethylene, with different levels induced at different times. Early biotic signaling in flg22-treated leaves and distal ones is an ethylene-dependent process that is also determined by the time of day and inhibited in the early night phase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Klaus-Peter Goetz, Frank-M Chmielewski, Danuse Tarkowska, Ales Pencik, Ondrej Novak
Summary: This study was conducted to assess the involvement of gibberellins (GAs), cytokinins (CKs), and auxins (AX) in the dormancy of sweet cherry buds. The results showed that these hormone groups did not exhibit significant changes during the transition between different dormancy phases. However, there were specific changes in the content of bioactive molecules during ontogenetic development.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Jana Bibova, Veronika Kabrtova, Veronika Vecerova, Zuzana Kucerova, Martin Hudecek, Lenka Plackova, Ondrej Novak, Miroslav Strnad, Ondrej Plihal
Summary: Plasmodiophora brassicae is a pathogen that causes clubroot disease in cruciferous plants, leading to morphological changes and symptoms such as gall formation and leaf abscission. The homeostasis of cytokinin and auxin appears to be crucial for club development. Study on late-stage infected Arabidopsis thaliana showed changes in cytokinin levels and their role in disease progression.
Letter
Plant Sciences
Federica Brunoni, Ales Pencik, Asta Zukauskaite, Anita Ament, Martina Kopecna, Silvio Collani, David Kopecny, Ondrej Novak
Article
Plant Sciences
Tomas M. Tessi, Veronica G. G. Maurino, Mojgan Shahriari, Esther Meissner, Ondrej Novak, Taras Pasternak, Benjamin S. S. Schumacher, Franck Ditengou, Zenglin Li, Jasmin Duerr, Noemi S. S. Flubacher, Moritz Nautscher, Alyssa Williams, Zuzanna Kazimierczak, Miroslav Strnad, Joerg-Oliver Thumfart, Klaus Palme, Marcelo Desimone, William D. D. Teale
Summary: An environmentally responsive root system is crucial for plant growth and crop yield, especially in suboptimal soil conditions. The protein AZG1, identified as a PIN1 interactor, is involved in auxin:cytokinin crosstalk and plays a role in regulating root system architecture in response to salt stress.
Article
Plant Sciences
Andrej Pavlovic, Ondrej Vrobel, Petr Tarkowski
Summary: In his book, Charles Darwin observed that the response of sundew plants to stimuli is not triggered by water drops but by dissolved chemicals or mechanical stimulation. This study used modern methods to investigate this 150-year-old mystery. The findings showed that sundew plants have a weak response to water drops but significant changes in enzyme activity and digestive enzyme abundance when exposed to other stimuli.
Article
Plant Sciences
Agata Daszkowska-Golec, Devang Mehta, R. Glen Uhrig, Agnieszka Braszewska, Ondrej Novak, Irene M. Fontana, Michael Melzer, Tomasz Plociniczak, Marek Marzec
Summary: We used a combination of transcriptomics, proteomics, and phytohormonomics analyses to investigate the SL-dependent mechanisms associated with water deficit response in barley. Our research revealed the role of SLs in alleviating the effects of water limitation on plants and identified potential strategies for developing drought-resistant barley.
Article
Developmental Biology
Elena A. Zemlyanskaya, Viacheslav Zemlianski, Ales Pencik, Dior R. Kelley, Yka Helariutta, Ondrej Novak, Kamil Ruzicka
Summary: Research has found that m6A modification can affect auxin-related defects and auxin signaling in Arabidopsis thaliana mutants, as well as lead to defects in endodermal patterning in the primary root.
Article
Plant Sciences
Batist Geldhof, Ondrej Novak, Bram van de Poel
Summary: Waterlogging-induced hypoxia in the root zone leads to adaptive responses in the shoot, such as leaf epinasty. This study analyzed the dynamics of multiple hormones in tomato leaves during waterlogging, revealing that leaf age determines hormone homeostasis and modulates changes during waterlogging. Early hormonal signals, including ethylene and abscisic acid, were found to induce epinasty, while the balance of indole-3-acetic acid and cytokinins in the petiole ultimately regulates differential growth.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Dorota Konickova, Katerina Mensikova, Katerina Klicova, Monika Chudackova, Michaela Kaiserova, Hana Prikrylova, Pavel Otruba, Martin Nevrly, Petr Hlustik, Eva Henykova, Michal Kaleta, David Friedecky, Radoslav Matej, Miroslav Strnad, Ondrej Novak, Lucie Plihalova, Raymond Rosales, Carlo Colosimo, Petr Kanovsky
Summary: Neurodegenerative diseases are a heterogeneous group of diseases that affect the nervous system. Clinical diagnosis of these diseases is challenging due to their variability and overlapping symptoms. This study aimed to identify biomarkers in the cerebrospinal fluid and serum that can aid in the differential diagnosis and differentiation of specific neurodegenerative diseases. The results suggest that the proposed biomarkers can be useful for distinguishing between certain types of neurodegenerative diseases.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Vaclav Mik, Tomas Pospisil, Federica Brunoni, Jiri Gruz, Vladimfra Nozkova, Claus Wasternack, Otto Miersch, Miroslav Strnad, Kristyna Flokova, Ondrej Novak, Jitka Siroka
Summary: This research presents a highly sensitive method for the reliable determination of seven OPDA-aa compounds in plants. The method was optimized for extraction, sample clean-up, and final analysis. Five endogenous (+)-OPDA-aa were identified and their levels were estimated in mechanically wounded Arabidopsis thaliana leaves, with a peak observed 60 minutes after the wounding. The synthetic and analytical methodologies developed in this study will contribute to the understanding of the biological significance of these metabolites in plants.
Article
Plant Sciences
Kristyna Bieleszova, Pavel Hladik, Martin Kubala, Richard Napier, Federica Brunoni, Zuzana Gelova, Lukas Fiedler, Ivan Kulich, Miroslav Strnad, Karel Dolezal, Ondrej Novak, Jiri Friml, Asta Zukauskaite
Summary: This study reports the synthesis of new NBD- or DNS-labelled IAA derivatives and investigates their biological activity, fluorescence properties, and subcellular accumulation patterns. These derivatives exhibit anti-auxin activity and may be useful for studying binding mechanisms and auxin-regulated processes.
PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Maira de Freitas Pereira, David Cohen, Lucas Auer, Nathalie Aubry, Marie-Beatrice Bogeat-Triboulot, Cyril Bure, Nancy L. Engle, Yves Jolivet, Annegret Kohler, Ondrej Novak, Iva Pavlovic, Pierrick Priault, Timothy J. Tschaplinski, Irene Hummel, Marie-Noelle Vaultier, Claire Veneault-Fourrey
Summary: Tree growth and survival rely on their ability to perceive and respond to environmental changes. This study found that ectomycorrhizal symbiosis can modify the signal responses of Populus trichocarpa to environmental changes and rewire molecular drivers of photosynthesis and the calcium signaling pathway. Additionally, ectomycorrhizas are less responsive to abiotic challenges compared to non-mycorrhizal roots.
Article
Plant Sciences
Vladimir Skalicky, Ioanna Antoniadi, Ales Pencik, Ivo Chamrad, Rene Lenobel, Martin F. Kubes, Marek Zatloukal, Asta Zukauskaite, Miroslav Strnad, Karin Ljung, Ondrej Novak
Summary: Auxin and cytokinin show subcellular distribution in Arabidopsis cells, forming hormone gradients. This finding provides important insights into the mechanisms of plant growth and development.
Article
Chemistry, Analytical
Ivan Petr, Ales Pencik, Jakub Styskala, Lenka Tranova, Petra Amakorova, Miroslav Strnad, Ondrej Novak
Summary: The study introduces a novel approach using ultra-high performance supercritical fluid chromatography coupled with tandem mass spectrometry (UHPSFC-MS/MS) for the quantification of plant hormones. Compared to traditional UHPLC-MS/MS methods, this new approach offers a shorter total run time and lower matrix effect.
ANALYTICA CHIMICA ACTA
(2024)
Article
Plant Sciences
Eva Luptakova, Armelle Vigouroux, Radka Koncitikova, Martina Kopecna, David Zalabak, Ondrej Novak, Sara Salcedo Sarmiento, Sanja Cavar Zeljkovic, David Jaroslav Kopecny, Klaus von Schwartzenberg, Miroslav Strnad, Lukas Spichal, Nuria De Diego, David Kopecny, Solange Morera
Summary: Cells save energy during nitrogen starvation by selectively autophagocytosing ribosomes and degrading RNA. The preference of plant NRHs for nucleosides influences their breakdown and has consequences for plant growth and metabolism.