期刊
NEW JOURNAL OF CHEMISTRY
卷 41, 期 9, 页码 3273-3279出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6nj04129a
关键词
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资金
- National Natural Science Funds for Distinguished Young Scholars [51625305]
- National Natural Science Foundation of China [51273187, 21374107, 21474097]
- Fundamental Research Funds of the Central Universities [WK2060200012]
Poly(amidoamine)s (PAMAMs) of high-generations (G > 5) exhibit high efficacy in gene transfection, but their high cost and severe cytotoxicity limit their use in biomedicine. In contrast, PAMAMs of low-generations (G < 3) are less toxic and easy to synthesize, but exhibit low transfection efficacy. In this report, we design a novel amphiphilic dendrimer by conjugating reactive oxygen species (ROS)-responsive poly(propylenesulfide) (PPS) to a low generation PAMAM dendrimer (G = 2, M-w = 3500 Da) via disulfide bonds. This amphiphilic conjugate can self-assemble into stable nanomicelles. The results show that the obtained nanomicelles have a high zeta potential, which can condense DNA tightly. Moreover, the DNA/nanomicelle polyplexes can be fully dissociated by intracellular ROS and easily release the entrapped DNA. As gene vectors, the nanomicelles exhibited high transfection efficacy and fairly low cytotoxicity in vitro.
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