Article
Biophysics
Pooja P. Kanade, Nomin-Erdene Oyunbaatar, Arunkumar Shanmugasundaram, Yun-Jin Jeong, Eung-Sam Kim, Bong-Kee Lee, Dong-Weon Lee
Summary: This study introduces a drug screening platform based on a microelectrode array integrated SU-8 cantilever, which enables simultaneous measurement of electrophysiology and contractility of cardiomyocytes. The feasibility of this platform is verified through drug tests, and it is found that some drugs have greater toxicity to contractility, highlighting the need for a reliable dual measurement platform for assessing cardiotoxicity.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Materials Science, Multidisciplinary
Jose de la Cruz, Diep Nguyen, Xavi Illa, Jessica Bousquet, Antonio P. Perez-Marin, Elena del Corro, Serge Picaud, Jose A. Garrido, Clement Hebert
Summary: Electroretinography (ERG) is a clinical test used to understand and diagnose various retinopathies. Traditional metal electrodes have limitations, while flexible electrodes made of graphene can overcome these limitations and perform multisite recording on the cornea.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biophysics
Diming Zhang, Yuting Xiang, Quchao Zou, Kai Zhu, Ning Hu
Summary: The assessment of drug cardiotoxicity using the electrical and mechanical properties of cardiomyocytes is crucial. Traditional recording strategies have limitations, but a new multimodal microelectrode biosensing system allows for simultaneous and dynamic monitoring of electromechanical signals from single cardiomyocytes.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Andrea Spanu, Annalisa Bonfiglio
Summary: Modern electrophysiology has advanced due to the development of sophisticated tools and materials, with the emergence of organic bioelectronics offering new advancements in cellular interfacing.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biochemistry & Molecular Biology
Miyu Okamoto, Kenta Shimba, Akihiro Kamono, Kiyoshi Kotani, Yasuhiko Jimbo
Summary: The study successfully developed a culture system to measure the spatial dynamics of reentry-like activity, revealing that a simple purification process is sufficient to induce reentry-like activity, which occurs mainly through changes in inter-beat intervals and conduction block for initiation and termination.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Engineering, Biomedical
Brianna Thielen, Ellis Meng
Summary: The review discusses the immune response to rigid neural probes and the development of flexible probes, as well as the challenges associated with inserting flexible probes. Maintaining insertion strategies is crucial for successful surgical placement.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Neurosciences
Tomoyo Isoguchi Shiramatsu, Kanato Mori, Kotaro Ishizu, Hirokazu Takahashi
Summary: The study demonstrated that visual MMN exhibited deviance detection properties, with the first-generation focus of visual MMN localized in the visual cortex. Additionally, cross-modal information processing influenced MMN, showing a non-linear relationship between single-modal and cross-modal MMNs.
FRONTIERS IN HUMAN NEUROSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Jin Song, Ashaq Ali, Yaohong Ma, Yiwei Li
Summary: In this study, a microfluidic system using a graphene-modified microelectrode array sensor was proposed for real-time monitoring of bacterial biofilms through electrochemical impedance. The results showed that increasing bacterial cell load on the sensing interface significantly prolonged the observation window period of the biofilm state, thereby improving the sensing signal quality. Simulation studies demonstrated that this method outperformed traditional endpoint detection in anti-biofilm drug screening.
NANOSCALE ADVANCES
(2023)
Article
Engineering, Biomedical
Tianshu Dong, Lei Chen, Paras R. Patel, Julianna M. Richie, Cynthia A. Chestek, Albert J. Shih
Summary: The machine described in this study automates the assembly of high-density carbon fiber (HDCF) arrays, eliminating the need for manual labor and improving accuracy and repeatability. The machine includes a roller-based carbon fiber extruder, a motion system with linear and rotary stages, an imaging system with digital microscope cameras, and a laser cutter. It is capable of precisely handling carbon fiber electrodes (CFEs) and accurately placing them on silicon support shanks. The use of this machine is significant for achieving fully automated HDCF array assembly and batch production.
JOURNAL OF NEURAL ENGINEERING
(2023)
Article
Engineering, Biomedical
Cort H. Thompson, Blake M. Evans, Dorothy X. Zhao, Erin K. Purcell
Summary: Implantation of electrodes in the brain can be used to record from or stimulate neural tissues to treat neurological disease and injury. Recent RNA-seq datasets identify genes associated with gliosis, neuronal function, myelination, and cellular metabolism following the insertion of microelectrodes. This study validates mRNA as a predictor of protein expression and provides insight into the spatiotemporal distribution of proteins in neural tissues after implantation.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Analytical
Zhixiang Zou, Zhongning Guo, Qinming Huang, Taiman Yue, Jiangwen Liu, Xiaolei Chen
Summary: Micro-electrical discharge machining (micro-EDM) is a promising method for processing micro-hole arrays for various applications. By analyzing wire vibration in the wire electro-discharge grinding (WEDG) system and developing an improved WEDG method, high-precision microelectrodes with the largest aspect ratio reported in the literature were successfully fabricated for in-process micro-EDM of hole array. Using response-surface experiments and in-process touch-measurement compensation strategy, accurate micro-hole arrays were produced on stainless steel sheets with minimal deviation.
Article
Chemistry, Analytical
Enhui He, Shengwei Xu, Guihua Xiao, Yuchuan Dai, Xinrong Li, Yilin Song, Fei Gao, Yu Zhang, Shihong Xu, Xinxia Cai
Summary: The special in vitro micro-electrode array (MEA) designed for rat hippocampal slices improved the accurate recording of electrical signals in the main cell layer, providing insights into the neural signal transmission pathway of epileptic discharges. The use of a less-hydrophilic-MWCNTs/PEDOT:PSS modification method significantly enhanced the performance of the MEA, making it a reliable and sensitive biosensor for hippocampal slices.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Electrochemistry
Hai-lan Chen, Guang-zhao Tian, Hao Yan, Song-xin Yang, Dong-Hwan Kim
Summary: By fabricating PEDOT nanotube array modified microelectrodes, this study successfully improved the electrical properties of neural electrodes, promoted cell adhesion and growth, and increased neurite extension and branching. In vitro experiments demonstrated that the PEDOT nanotube arrays provided good support for cells, even without the presence of other cell adhesion-promoting molecules.
ELECTROCHIMICA ACTA
(2022)
Article
Multidisciplinary Sciences
Mohammadali Sharifshazileh, Karla Burelo, Johannes Sarnthein, Giacomo Indiveri
Summary: The study introduces an embedded device that can process intracranial EEG from epilepsy patients to detect High Frequency Oscillations in real-time. By utilizing mixed-signal neuromorphic circuits, a neural network processing system achieves state-of-the-art accuracy, sensitivity, and specificity in HFO detection.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Keerakit Kaewket, Pattanaphong Janphuang, Peerawat Laohana, Nantawat Tanapongpisit, Wittawat Saenrang, Kamonwad Ngamchuea
Summary: In this study, silver microelectrode arrays were fabricated using photolithography for the analysis of H2O2 in low ionic strength samples. The developed sensor showed excellent repeatability and reproducibility, and could detect H2O2 directly without the addition of supporting electrolyte.
Review
Chemistry, Multidisciplinary
Wendel Wohlleben, Annette Mehling, Robert Landsiedel
Summary: Chemicals can be assessed for hazard in groups based on their similar structures and toxicological properties, similar to how elements are grouped in the periodic system. Strategies for chemical grouping have been used to support the evaluation of human health hazards, exposures, and risks, as shown in case studies. Structural similarity is commonly used as the basis for grouping, but it may not always be sufficient. Ten principles for grouping have been derived, including transparency, adequacy of methods, and consideration of toxicological properties, which can facilitate effective risk management.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Medicine, Legal
Susanne N. Kolle, Melanie Flach, Marcus Kleber, David A. Basketter, Britta Wareing, Annette Mehling, Lars Hareng, Nico Watzek, Steffen Bade, Dorothee Funk-Weyer, Robert Landsiedel
Summary: In the past decade, several non-animal methods have been adopted as OECD test guidelines to identify skin sensitization hazards. However, the predictive accuracy of these methods for substances beyond the chemical domains of individual validation studies remains largely untested. This study compares the skin sensitization test results from in vitro and in chemico methods to available in vivo data for plant extracts and polymeric materials.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2023)
Biographical-Item
Toxicology
Jan G. Hengstler, Hermann Bolt, Uwe Heinrich, Andrea Hartwig, Robert Landsiedel
ARCHIVES OF TOXICOLOGY
(2023)
Correction
Toxicology
Jan G. Hengstler, Hermann Bolt, Uwe Heinrich, Andrea Hartwig, Robert Landsiedel
ARCHIVES OF TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dunja Dimitrijevic, Eric Fabian, Dorothee Funk-Weyer, Robert Landsiedel
Summary: In pharmacokinetics, plasma protein binding (PPB) is an important parameter affecting drug metabolism. The unbound fraction (fu) is considered the effective concentration at the target site. In vitro models, such as physiologically based toxicokinetic models (PBTK), are increasingly used in pharmacology and toxicology. This study compared three methods (rapid equilibrium dialysis, ultrafiltration, and ultracentrifugation) to quantify fu and found that the selection of the separation method should be based on the properties of the test substance. Rapid equilibrium dialysis was suitable for a broader range of substances, while ultrafiltration and ultracentrifugation were suitable for polar substances.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Sabina Ramirez-Hincapie, Barbara Birk, Philipp Ternes, Varun Giri, Volker Haake, Michael Herold, Franziska Maria Zickgraf, Andreas Verlohner, Hans-Albrecht Huener, Hennicke Kamp, Peter Driemert, Robert Landsiedel, Elke Richling, Dorothee Funk-Weyer, Bennard van Ravenzwaay
Summary: Cell-based metabolomics provides multiparametric readouts for decision making in compound development. A LC-MS/MS-based metabolomics platform was developed for liver toxicity classification in HepG2 cells. The platform showed dose response and differentiation between liver toxicity modes of action, and identified specific metabolite patterns for each mode of action. This method offers a mechanistic-based, cost-effective screening for compound development pipelines.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Toxicology
Sabina Ramirez-Hincapie, Barbara Birk, Philipp Ternes, Varun Giri, Franziska Maria Zickgraf, Volker Haake, Michael Herold, Hennicke Kamp, Peter Driemert, Robert Landsiedel, Elke Richling, Dorothee Funk-Weyer, Bennard van Ravenzwaay
Summary: This study applied a high-throughput LC-MS/MS-based broad targeted metabolomics system to investigate the metabolic dynamics of nitrofurantoin over time and concentration and derived a mechanistic-anchored approach for point of departure (PoD) derivation. The results showed clear time and concentration effects on the metabolome of HepG2 cells. This approach allows for the derivation of PoD that can be mechanistically anchored to established key events and contribute to the development of new adverse outcome pathways.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pia Wilhelmi, Varun Giri, Franziska Maria Zickgraf, Volker Haake, Stefan Henkes, Peter Driemert, Paul Michaelis, Wibke Busch, Stefan Scholz, Burkhard Flick, Marta Barenys, Barbara Birk, Hennicke Kamp, Robert Landsiedel, Dorothee Funk-Weyer
Summary: Through LC-MS/MS and GC-MS metabolomics, this study investigated the changes in endogenous metabolites in zebrafish embryos as indicators of developmental toxicity pathways. The results showed that exposure to the compound PTU led to morphological alterations and significant metabolic changes, which could be linked to the inhibition of thyroid peroxidase and potential neurodevelopmental impairments. This study demonstrated that metabolite changes in zebrafish embryos provide mechanistic information associated with the mode of action of PTU.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Engineering, Biomedical
Giulia Parodi, Martina Brofiga, Vito Paolo Pastore, Michela Chiappalone, Sergio Martinoia
Summary: The study investigates the impact of the balance between excitation and inhibition on the development of human neuronal networks. The researchers used homogeneous and heterogeneous cultures with controlled E/I ratios to observe the electrophysiological activity. They found that increased inhibition in the heterogeneous cultures affected the duration and organization of bursts and network activity. The study demonstrates the feasibility of an in vitro model for investigating neurodevelopmental disorders where the E/I balance is impaired.
JOURNAL OF NEURAL ENGINEERING
(2023)
Article
Medicine, Legal
Jens C. Otte, Heli Miriam Hollnagel, Christiane Nagel, Renata Friederike Gerhardt, Wendel Wohlleben, Nathalie Vallotton, Diederik Schowanek, Gordon Sanders, Joe M. Frasca, Tushar Mahale, Mark Pemberton, Bjoern Hidding, Robert Landsiedel
Summary: This study proposes a three-tiered regulatory approach for assessing polymers, which includes grouping the polymers based on their chemistry, physico-chemical properties, and hazard similarity. By reducing animal testing and providing sufficient data, this approach aims to achieve an adequate level of protection.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Toxicology
Franziska M. Zickgraf, Aishwarya Murali, Robert Landsiedel
Summary: This article summarizes studies on the effects of engineered nanomaterials (ENM) on the gut microbiome, showing that ENM can affect the microbiome in various ways, although few studies demonstrate a link to adverse effects on the host. Based on these findings, future studies can assess the hazards and risks of ENM through changes in the microbiome.
CURRENT OPINION IN TOXICOLOGY
(2023)
Correction
Environmental Sciences
Johannes G. Keller, Michael Persson, Philipp Mueller, Lan Ma-Hock, Kai Werle, Josje Arts, Robert Landsiedel, Wendel Wohlleben
Proceedings Paper
Engineering, Ocean
Antonio Novellino, Flavio Augusto Bastos da Cruz Martins, Gabriele Pieri, Francesco Misurale
Summary: This paper provides an overview of the existing best practices and quality indices for the most important platforms, initiatives, and projects in the field of NAUTILOS - New Approach to Underwater Technologies for Innovative, Low-cost Ocean observation - H2020 project. Governments and the scientific community have made efforts to encourage standardization and best practices for data collection, processing, and distribution.
2022 IEEE INTERNATIONAL WORKSHOP ON METROLOGY FOR THE SEA LEARNING TO MEASURE SEA HEALTH PARAMETERS (METROSEA)
(2022)
Proceedings Paper
Engineering, Ocean
Antonio Novellino, Haizea Jimenez, Gabriele Pieri, Silvia Merlino, Mariana Machado Toffolo, Eva Chatzinikolaou, Giuseppe Suaria, Stefano Goffredo, Sandra Granchinho, Luca Tixi, Fazuoli, Sandra Sa
Summary: This paper focuses on knowledge co-production through the sharing of experiences, major outcomes, and good practices in various citizen science projects. Specifically, it gathers inputs from the European Marine Days 2022 - EU4Ocean workshop on citizens' empowerment through ocean knowledge co-production and examines the gaps and needs between Science, Policy, and Citizens.
2022 IEEE INTERNATIONAL WORKSHOP ON METROLOGY FOR THE SEA LEARNING TO MEASURE SEA HEALTH PARAMETERS (METROSEA)
(2022)
Proceedings Paper
Engineering, Ocean
Antonio Novellino, Francesco Misurale, Christian Autermann, Patrick Gorringe, Marco Alba, Dick Schaap, Enrico Boldrini, Sissy Iona
Summary: This paper presents the approach, standards, and tools developed to support data providers in implementing operational data interoperability with EMODnet.
2022 IEEE INTERNATIONAL WORKSHOP ON METROLOGY FOR THE SEA LEARNING TO MEASURE SEA HEALTH PARAMETERS (METROSEA)
(2022)