期刊
NEUROSCIENCE RESEARCH
卷 135, 期 -, 页码 21-31出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neures.2017.12.007
关键词
Skin derived precursor-Schwann cells (SKP-SCs); Acellular matrix (ACM); Chitosan/Silk fibroin; Tissue engineering nerve graft; Peripheral nerve repair
资金
- National Natural Science Foundation of China [81471255]
- Basic Research Program of Jiangsu Education Department [14KJA310004]
- National Key Basic Research Program of China [2014CB542202]
- National Key Research and Development Program of China [2016YFC1101600]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Extracellular/acellular matrix has been attracted much research interests for its unique biological characteristics, and ACM modified neural scaffolds shows the remarkable role of promoting peripheral nerve regeneration. In this study, skin-derived precursors pre-differentiated into Schwann cells (SKP-SCs) were used as parent cells to generate acellular(ACM) for constructing a ACM-modified neural scaffold. SKP-SCs were co-cultured with chitosan nerve guidance conduits (NGC) and silk fibroin filamentous fillers, followed by decellularization to stimulate ACM deposition. This NGC-based, SKP-SC-derived ACM-modified neural scaffold was used for bridging a 10 mm long rat sciatic nerve gap. Histological and functional evaluation after grafting demonstrated that regenerative outcomes achieved by this engineered neural scaffold were better than those achieved by a plain chitosan-silk fibroin scaffold, and suggested the benefits of SKP-SC-derived ACM for peripheral nerve repair. (C) 2017 Elsevier Ireland Ltd and Japan Neuroscience Society. All rights reserved.
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