Article
Biochemistry & Molecular Biology
Dariia O. Dryn, Mariia I. Melnyk, Donal Melanaphy, Igor V. Kizub, Christopher D. Johnson, Alexander V. Zholos
Summary: This study demonstrates for the first time the biphasic contractile reaction of TRPV4 in rat pulmonary artery, showing relaxation followed by a contraction. The relaxation phase is induced by BKCa activation, while the subsequent contraction is mediated by TRPV4-dependent depolarization and Ca-L activation. Similar changes in membrane potential are observed with the activation of TRPM8 in the rat tail artery.
Review
Biochemistry & Molecular Biology
Dorina Zoephel, Chantal Hof, Annette Lis
Summary: Aging is an unstoppable process that affects every cell of the organism, especially the changes in Ca2+ signaling in immune cells which may profoundly impact the development of age-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Jonas Tjaden, Annika Eickhoff, Sarah Stahlke, Julian Gehmeyr, Matthias Vorgerd, Verena Theis, Veronika Matschke, Carsten Theiss
Summary: This study investigated the impact of VEGF on T-type Ca2+ channels, showing that VEGF stimulation increases the mRNA expression of Cav3.1, Cav3.2, and Cav3.3, indicating a role of VEGF in neuronal plasticity.
Article
Biochemistry & Molecular Biology
Isis J. Amaye, Patrice L. Jackson-Ayotunde, Miguel Martin-Caraballo
Summary: Trifluoromethylated N-benzamide enaminones have shown potential as anticonvulsants for drug-resistant epilepsy. They target T-type Ca2+ channels, with two meta-trifluoromethyl N-benzamide enaminones inhibiting these channels. The lead analogs of these compounds affect the gating mechanism of Cav3.3 T-type Ca2+ channels, while altering the steady-state inactivation of Cav3.2. This research provides insights for the development of specific blockers for T-type Ca2+ channels in the treatment of epileptic seizures.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Review
Physiology
Galina Yu Mironova, Nadia Haghbin, Donald G. G. Welsh
Summary: This article discusses the mechanistic foundation of vascular smooth muscle contraction, including membrane potential, opening of L-type calcium channels, and intracellular rise in Ca2+. It also explores the functional coupling and cooperative behavior of neighboring channels, as well as the impact of channel trafficking on cytoskeleton dynamics.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Ludovica Filippini, Nadine J. J. Ortner, Teresa Kaserer, Jorg Striessnig
Summary: Voltage-gated L-type Ca2+-channels, especially Ca(v)1.3 subtype, are potential drug targets for the treatment of various diseases. However, the selectivity of Ca(v)1.3-selective blockers remains controversial, and there are no suitable pharmacological tools to confirm or refute the role of Ca(v)1.3 channels in cellular responses. Essential criteria for a small molecule to be considered Ca(v)1.3-selective are suggested.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Roman Y. Medvedev, Jose L. Sanchez-Alonso, Catherine A. Mansfield, Aleksandra Judina, Alice J. Francis, Christina Pagiatakis, Natalia Trayanova, Alexey V. Glukhov, Michele Miragoli, Giuseppe Faggian, Julia Gorelik
Summary: The study revealed structural and functional alterations in RV myocytes in heart failure, including cellular hypertrophy, disruption of tubular network, enhanced contractility, and abnormal Ca2+ release activity. These changes are specifically localized in microdomains, potentially leading to compensatory RV remodelling and arrhythmogenic risk in the setting of LV failure.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Andrei N. Tsentsevitsky, Eduard F. Khaziev, Irina V. Kovyazina, Alexey M. Petrov
Summary: The study found that GIRK channels can regulate the timing of neurotransmitter release and act as a positive modulator of synaptic vesicle exocytosis in frog motor nerve terminals, partially through L-type calcium channels. Additionally, GIRK channels are key players in the feedback control of neurotransmitter release by muscarinic acetylcholine receptors.
Article
Multidisciplinary Sciences
Nicholas C. Vierra, Samantha C. O'Dwyer, Collin Matsumoto, L. Fernando Santana, James S. Trimmer
Summary: In mammalian brain neurons, membrane depolarization triggers Ca2+ influx through voltage-gated Ca2+ channels, leading to diverse cellular responses, including gene expression. The L-type Ca2+ channels in neuronal soma and dendrites play a key role in this process. The organization of signaling complexes by Kv2.1 at endoplasmic reticulum-plasma membrane junctions enhances the clustering and activity of Cav1.2 channels, impacting somatic Ca2+ signals and excitation-transcription coupling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Vladislav Chubinskiy-Nadezhdin, Svetlana Semenova, Valeria Vasileva, Alla Shatrova, Natalia Pugovkina, Yuri Negulyaev
Summary: Endometrial mesenchymal stem cells (eMSCs) have the ability to differentiate into different cell types and are influenced by mechanical forces in their microenvironment. This study identified Piezo1 proteins as mechanosensitive channels in the plasma membrane of eMSCs, which transduce mechanical stimuli into intracellular signaling pathways. The influx of Ca2+ triggered by Piezo1 activity is regulated by store-operated Ca2+ entry (SOCE) through the ORAI1 channel and STIM1/STIM2 Ca2+ sensors. Activation of Piezo1 or SOCE does not cause cytotoxicity in eMSCs but affects their migratory capacity and cell proliferation. This finding suggests that Piezo1 and SOCE play important roles in the regulation of intracellular calcium, which influences the migratory activity and regenerative potential of eMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Aiwen Xu, Michael Beyeler
Summary: This study used a biophysical model to simulate the retina and investigated the impact of neuroanatomical changes on cellular responses during retinal degeneration. The findings revealed that retinal cell activity increased and electrical stimulation thresholds also increased during retinal degeneration, and testable predictions about the underlying neuroanatomical mechanisms were proposed.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Chemistry, Medicinal
Muqing Zhang, Jiaying Qi, Qianqian He, Donglai Ma, Jing Li, Xi Chu, Saijie Zuo, Yixin Zhang, Li Li, Li Chu
Summary: Liquiritigenin (Lq) protects against myocardial ischemic injury by reducing intracellular calcium concentration through antioxidation, antiapoptosis, counteraction mitochondrial dysfunction, and inhibition of ICa-L.
PHYTOTHERAPY RESEARCH
(2022)
Article
Medicine, Research & Experimental
Yuan Zhang, Zhiyuan Qian, Dongsheng Jiang, Yufang Sun, Shangshang Gao, Xinghong Jiang, Hua Wang, Jin Tao
Summary: The study revealed that Nmb stimulates Cav3.2 channels through NmbR via a G(beta gamma)-dependent AMPK/PKA pathway, leading to hyperexcitability of TG neurons and pain hypersensitivity in mouse models.
Article
Integrative & Complementary Medicine
Katesirin Ruamyod, Wattana B. Watanapa, Chanrit Kakhai, Pimchanok Nambundit, Sukrit Treewaree, Parin Wongsanupa
Summary: This study aimed to investigate the effect of ferulic acid, a natural compound, on pancreatic beta cell viability, Ca2+ channels, and insulin secretion. It was found that ferulic acid did not affect cell viability and rapidly increased L-type Ca2+ channel current, while having no effect on T-type Ca2+ channels. Ferulic acid also significantly increased insulin secretion, which was mediated by augmenting Ca2+ influx through L-type Ca2+ channels. This study provides the first electrophysiological demonstration of the effects of acute ferulic acid treatment on pancreatic beta cells.
JOURNAL OF INTEGRATIVE MEDICINE-JIM
(2023)
Article
Biology
Andre G. Kamkin, Olga V. Kamkina, Viktor E. Kazansky, Vadim M. Mitrokhin, Andrey Bilichenko, Elizaveta A. Nasedkina, Stanislav A. Shileiko, Anastasia S. Rodina, Alexandra D. Zolotareva, Valentin I. Zolotarev, Pavel V. Sutyagin, Mitko I. Mladenov
Summary: This study identified specific ion channels and their potential role in regulating ionic currents in rat ventricular cardiomyocytes in response to mechanical stimulation. TRPM7, TRPC1, and TRPM4 channels were found to be mechanosensitive, while Ca(V)1.2 channels showed mechanosensitive properties. These findings provide insights into the molecular mechanisms underlying stretch-induced responses in rat ventricular myocytes and have implications for understanding cardiac physiology and pathophysiology.
Correction
Neurosciences
Lucia Privitera, Ellen L. Hogg, Matthias Gaestel, Mark J. Wall, Sonia A. L. Correa
Article
Neurosciences
Li-Ya Jiang, Guan-Hao Wang, Jing-Jiao Xu, Xiao-Li Li, Xiao-Yan Lin, Xiang Fang, Hong-Xu Zhang, Mei Feng, Chun-Ming Jiang
Summary: This study reveals the importance of LINC00473 in regulating temozolomide (TMZ) resistance in glioblastoma (GB) and its potential mechanism. By regulating the expression of CEBP alpha and MGMT, LINC00473 promotes the formation of chemoresistance. Furthermore, LINC00473 can transfer chemoresistance to adjacent sensitive cells through exosomes.
Article
Neurosciences
Olga Kopach, Tetyana Pivneva, Nataliya Fedirko, Nana Voitenko
Summary: This study found that diabetic animals exhibit severe xerostomia characterized by reduced saliva flow rate, diminished total protein content, and decreased amylase activity. The impaired saliva production in diabetes is associated with reduced and delayed intracellular Ca2+ signals in submandibular acinar cells, caused by malfunctioning mitochondria. Targeting malfunctioning mitochondria may be a potential strategy for the treatment of diabetic xerostomia.
Article
Neurosciences
Nicholas M. Timme, Cherish E. Ardinger, Seth D. C. Weir, Rachel Zelaya-Escobar, Rachel Kruger, Christopher C. Lapish
Summary: This study aimed to assess aversion-resistant drinking behavior in head-fixed mice and explore the relationship between non-consummatory behaviors and aversion-resistant drinking. The results showed that head-fixed mice exhibited heterogenous levels of aversion-resistant drinking and non-consummatory behaviors were related to the intensity of this behavior.
Article
Neurosciences
David R. Maguire, Charles P. France
Summary: Methocinnamox (MCAM) is a novel, long-acting opioid receptor antagonist that effectively decreases fentanyl self-administration and prevents opioid overdose in monkeys. The study demonstrates the potential therapeutic utility of MCAM in the treatment of opioid use disorder.
Article
Neurosciences
Xiang Li, Dan Feng, Shenglu Ma, Mingxing Li, Shulei Zhao, Man Tang
Summary: This study investigated the effects of fluoxetine on neurochemical, neurobiological, and neurobehavioral changes in different subregions of the hippocampus. The results showed that fluoxetine increased dialysate 5-HT, decreased membrane 5-HTT protein, and increased cytoplasmic fraction. Additionally, fluoxetine reduced immobility times in behavioral tests, with greater effects observed in the ventral subregion compared to the dorsal subregion.
Article
Neurosciences
Alexander V. Zholos, Mariia I. Melnyk, Dariia O. Dryn
Summary: Acetylcholine is an important neurotransmitter in visceral smooth muscles, activating M2 and M3 muscarinic receptors to cause smooth muscle excitation and contraction. This review focuses on the cellular and molecular mechanisms underlying acetylcholine-induced depolarisation and smooth muscle contraction, as well as the effects of anticholinergic drugs on gastrointestinal motility. The knowledge gained from recent studies has greatly expanded our understanding of these processes.
Article
Neurosciences
Zhenlong Li, Hsien-Yu Peng, Chau-Shoun Lee, Tzer-Bin Lin, Ming-Chun Hsieh, Cheng-Yuan Lai, Han-Fang Wu, Lih-Chyang Chen, Mei-Ci Chen, Dylan Chou
Summary: Methylone shows significant efficacy in treating depression and social deficits, making it an ideal candidate for anti-depressant medication.
Article
Neurosciences
Aline Freyssin, Allison Carles, Sarra Guehairia, Gilles Rubinstenn, Tangui Maurice
Summary: This study explores the potential of combining FENM and S1R agonists in the treatment of Alzheimer's disease. The results showed that most FENM-based combinations can protect against learning deficits caused by A beta 25-35, with better efficacy in short-term memory.
Article
Neurosciences
J. D. Lorente, J. Cuitavi, L. Rullo, S. Candeletti, P. Romualdi, L. Hipolito
Summary: This study analyzed the effects of pain on negative affect in different sexes and time courses, as well as the involvement of the dynorphinergic and corticotropin releasing factor systems in these pain-related behaviors. The results showed sex and time-dependent anxiety- and anhedonia-like behaviors induced by pain in female rats. The recruitment of KOR/DYN in the NAc was identified as a key neurological substrate mediating pain-induced behavioral alterations.
Article
Neurosciences
Rongjun Liu, Daofan Sun, Xiuzhong Xing, Qingge Chen, Bo Lu, Bo Meng, Hui Yuan, Lan Mo, Liufang Sheng, Jinwei Zheng, Qiusheng Wang, Junping Chen, Xiaowei Chen
Summary: The coexistence of pain and depression is frequently observed in patients with chronic pain and depression. Oxytocin, a neuropeptide, has been reported to relieve chronic pain and depressive symptoms. This study investigated the effect of intranasal oxytocin on neuropathic pain and comorbid depressive symptoms, and found that oxytocin attenuated depression-like behavior but did not alleviate mechanical hyperalgesia. The results suggest that intranasal oxytocin may have the potential to treat depressive symptoms in neuropathic pain patients.