Article
Biochemical Research Methods
William M. Stoy, Bo Yang, Ali Kight, Nathaniel C. Wright, Peter Y. Borden, Garrett B. Stanley, Craig R. Forest
Summary: The new method utilizes the dependency on the distance between cell membrane and glass pipette to develop an autonomous synchronization method for high-yield whole-cell recordings in the deep brain of living mice. This synchronization nearly doubles the gigaseal yield and significantly improves whole-cell recording yield, making pan-brain whole-cell electrophysiology practical in the living mouse brain.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Multidisciplinary Sciences
Zeinab Jahed, Yang Yang, Ching-Ting Tsai, Ethan P. Foster, Allister F. McGuire, Huaxiao Yang, Aofei Liu, Csaba Forro, Zen Yan, Xin Jiang, Ming-Tao Zhao, Wei Zhang, Xiao Li, Thomas Li, Annalisa Pawlosky, Joseph C. Wu, Bianxiao Cui
Summary: This study develops mechanically robust nanocrown electrodes with high success rates achieved through electroporation, and validates their accurate recording of intracellular action potentials through simultaneous patch clamp recording. The study also demonstrates the ability of nanocrown electrodes to enable prolonged recording of the same cells after sequential addition of drugs. This technological advancement is significant for establishing a screening assay for preclinical evaluation of drug-induced arrhythmogenicity.
NATURE COMMUNICATIONS
(2022)
Article
Instruments & Instrumentation
Ian Delgadillo Bonequi, Abraham Stroschein, Lucas J. Koerner
Summary: This article describes a custom and open-source FPGA-based data acquisition system that is used for electrophysiology and closed-loop feedback experiments. The system combines FPGA acquisition and processing with high-speed analog and digital converters to enable real-time feedback. The digital approach simplifies experimental setup and repeatability by allowing for system identification and in situ tuning of filter bandwidths.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
Article
Engineering, Electrical & Electronic
Fatemeh Ansari, Mohammad Yavari
Summary: In this paper, a chopper capacitively-coupled instrumentation amplifier (CCIA) with high input impedance and low input-referred noise is proposed for closed-loop neural recording. The proposed amplifier features a recycling folded-cascode amplifier with an inherent common-mode feedback (CMFB) circuit and a fully-differential common-source amplifier to tolerate large common-mode and differential in-band artifacts and achieve a large output signal swing. The amplifier also reduces total harmonic distortion (THD) and improves noise efficiency factor (NEF) through the use of small capacitors and lower cut-off frequency. The proposed amplifier has been simulated using TSMC 180 nm CMOS process with satisfactory results.
CIRCUITS SYSTEMS AND SIGNAL PROCESSING
(2022)
Article
Biochemical Research Methods
Christopher Miranda, Madeleine R. Howell, Joel F. Lusk, Ethan Marschall, Jarrett Eshima, Trent Anderson, Barbara S. Smith
Summary: This study demonstrates a method of guiding glass micropipette electrodes to neurons by collecting fluorescence at the aperture using an intra-electrode tapered optical fiber. The use of a tapered fiber for excitation and collection of fluorescence at the micropipette tip allows for targeted robotic approach to labeled neurons independent of microscopy.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Multidisciplinary Sciences
Jerome Montnach, Maxime Lorenzini, Adrien Lesage, Isabelle Simon, Sebastien Nicolas, Eleonore Moreau, Celine Marionneau, Isabelle Baro, Michel De Waard, Gildas Loussouarn
Summary: The whole-cell voltage-clamp technique requires consideration of the impact of current amplitude on data accuracy, especially in high throughput patch-clamp applications to prevent inaccuracies. Mathematical kinetic models can predict how large current amplitudes and series resistance artifacts affect voltage-dependent activation and inactivation processes, providing simple guidelines for practice.
SCIENTIFIC REPORTS
(2021)
Review
Pharmacology & Pharmacy
Shirin Ahmadi, Melisa Benard-Valle, Kim Boddum, Fernanda C. Cardoso, Glenn F. King, Andreas Hougaard Laustsen, Anne Ljungars
Summary: Ion channels are crucial for various physiological processes. Venomous creatures exploit ion channels by producing toxins that target them, leading to dysregulation which can cause severe complications in humans. However, the discovery of selective venom toxins has contributed to the development of drugs for envenoming and ion channel-related diseases.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Tijmen S. Bos, Jim Boelrijk, Stef R. A. Molenaar, Brian van 't Veer, Leon E. Niezen, Denice van Herwerden, Saer Samanipour, Dwight R. Stoll, Patrick Forre, Bernd Ensing, Govert W. Somsen, Bob W. J. Pirok
Summary: In this study, a novel open-source algorithm called AutoLC was developed for automated and interpretive method development of liquid chromatography. The algorithm was tested and showed that retention modeling is more efficient while Bayesian optimization is more flexible but limited in scalability.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Hong-Viet V. Ngo, Bernhard P. Staresina
Summary: Sleep is a privileged state for memory reactivation and consolidation. This research combines the use of reminder cues and brain stimulation to control the timing of memory reactivation. The results show that providing cues during specific sleep stages can significantly reduce overnight forgetting and enhance memory recall.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Bo Qin, Siyuan Liu, Jiang-Fei Xu
Summary: In this study, a closed-loop recycling approach using reversible amidation chemistry is presented for carbon fiber reinforced polymer composites. This method enables the complete recovery of intact carbon fibers and pure monomers, and allows the manufacture of high-performance and sustainable composites, offering significant environmental and economic benefits.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Jokubas Ausra, Micah Madrid, Rose T. Yin, Jessica Hanna, Suzanne Arnott, Jaclyn A. Brennan, Roberto Peralta, David Clausen, Jakob A. Bakall, Igor R. Efimov, Philipp Gutruf
Summary: Monitoring and control of cardiac function are crucial for studying cardiovascular pathophysiology and developing treatments. However, it is currently challenging to control the heart in freely moving small animal subjects. This study introduces a wireless battery-free device that enables real-time cardiac control and multisite stimulation, allowing optogenetic modulation of the entire rodent heart.
Article
Biochemistry & Molecular Biology
Farhad Jahanfar, Laura Sadofsky, Alyn Morice, Massimo D'Amico
Summary: TRPM8 is an important ion channel involved in various diseases. This study identified two potential TRPM8 inhibitors through high-throughput screening, providing candidate drugs for further development.
Article
Biochemistry & Molecular Biology
Juan Facundo Chrestia, Maria del Carmen Esandi, Cecilia Bouzat
Summary: Cannabidiol (CBD), a terpenoid compound from marijuana, has attracted pharmaceutical interest for its potential therapeutic uses. This study reveals the molecular mechanisms by which CBD modulates the function of the alpha 7 nicotinic acetylcholine receptor (alpha 7 nAChR), which is involved in various pathological conditions. CBD inhibits alpha 7 nAChR activity and decreases intracellular calcium responses, providing insights into its pharmacological relevance.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Engineering, Electrical & Electronic
Xianli Wang, Yajun Yu, Yichuan Dai, Qianming Xu, Kaiqin Chu, Zachary J. Smith
Summary: The low resolution Raman concept allows for a low-cost and automated Raman microscope capable of analyzing thousands of microscopic objects without user intervention. By sacrificing spectral resolution, the need for high quality equipment is eliminated, making it a promising tool for pervasive Raman spectroscopy.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Article
Engineering, Biomedical
Martin A. Garenfeld, Nikola Jorgovanovic, Vojin Ilic, Matija Strbac, Milica Isakovic, Jakob L. Dideriksen, Strahinja Dosen
Summary: The study proposed a novel compact solution for simultaneous recording and stimulation through dynamic blanking of stimulation artefacts. By developing a feedback coding scheme specifically for myoelectric control, it was found that the quality of closed-loop control was preserved in spite of electrotactile stimulation.
JOURNAL OF NEUROENGINEERING AND REHABILITATION
(2021)