The mystery of membrane organization: composition, regulation and roles of lipid rafts

Self-organizing actin patterns shape membrane architecture but not cell mechanics

(2017) M. Fritzsche et al.   Nature Communications

Protein sorting by lipid phase-like domains supports emergent signaling function in B lymphocyte plasma membranes

(2017) Matthew B Stone et al.   eLife

Closing the gap: The approach of optical and computational microscopy to uncover biomembrane organization

(2016) Christian Eggeling et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Polyunsaturated Lipids Regulate Membrane Domain Stability by Tuning Membrane Order

(2016) Kandice R. Levental et al.   BIOPHYSICAL JOURNAL

Cortical actin and the plasma membrane: inextricably intertwined

(2016) Darius V Köster et al.   CURRENT OPINION IN CELL BIOLOGY

Lipid rafts in immune signalling: current progress and future perspective

(2016) Pallavi Varshney et al.   IMMUNOLOGY

The Continuing Mystery of Lipid Rafts

(2016) Ilya Levental et al.   JOURNAL OF MOLECULAR BIOLOGY

Dynamic Reorganization and Correlation among Lipid Raft Components

(2016) Mónica M. Lozano et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Remodeling of the postsynaptic plasma membrane during neural development

(2016) Karolina Tulodziecka et al.   MOLECULAR BIOLOGY OF THE CELL

Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane

(2016) Takahiro K. Fujiwara et al.   MOLECULAR BIOLOGY OF THE CELL

Raft-based interactions of gangliosides with a GPI-anchored receptor

(2016) Naoko Komura et al.   Nature Chemical Biology

Actomyosin dynamics drive local membrane component organization in an in vitro active composite layer

(2016) Darius Vasco Köster et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Actin kinetics shapes cortical network structure and mechanics

(2016) M. Fritzsche et al.   Science Advances

Nanoscopic substructures of raft-mimetic liquid-ordered membrane domains revealed by high-speed single-particle tracking

(2016) Hsiao-Mei Wu et al.   Scientific Reports

Solvatochromic Nile Red Probes with FRET Quencher Reveal Lipid Order Heterogeneity in Living and Apoptotic Cells

(2015) Rémy Kreder et al.   ACS Chemical Biology

Nanoclusters of the resting T cell antigen receptor (TCR) localize to non-raft domains

(2015) Katharina Beck-García et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

The Effect of Membrane Lipid Composition on the Formation of Lipid Ultrananodomains

(2015) Priyadarshini Pathak et al.   BIOPHYSICAL JOURNAL

Hexagonal Substructure and Hydrogen Bonding in Liquid-Ordered Phases Containing Palmitoyl Sphingomyelin

(2015) Alexander J. Sodt et al.   BIOPHYSICAL JOURNAL

MPP1 as a Factor Regulating Phase Separation in Giant Plasma Membrane-Derived Vesicles

(2015) Joanna Podkalicka et al.   BIOPHYSICAL JOURNAL

Transbilayer Lipid Interactions Mediate Nanoclustering of Lipid-Anchored Proteins

(2015) Riya Raghupathy et al.   CELL

Structural determinants of protein partitioning into ordered membrane domains and lipid rafts

(2015) Joseph Helmuth Lorent et al.   CHEMISTRY AND PHYSICS OF LIPIDS

Spectral Imaging to Measure Heterogeneity in Membrane Lipid Packing

(2015) Erdinc Sezgin et al.   CHEMPHYSCHEM

Super-resolution optical microscopy of lipid plasma membrane dynamics

(2015) Christian Eggeling   Essays in Biochemistry

Involvement of glycosphingolipid-enriched lipid rafts in inflammatory responses

(2015) Kazuhisa Iwabuchi   Frontiers in Bioscience-Landmark

Lipid raft–dependent plasma membrane repair interferes with the activation of B lymphocytes

(2015) Heather Miller et al.   JOURNAL OF CELL BIOLOGY

Analysis of lipid-composition changes in plasma membrane microdomains

(2015) Hideo Ogiso et al.   JOURNAL OF LIPID RESEARCH

ABCB4 exports phosphatidylcholine in a sphingomyelin-dependent manner

(2015) Yu Zhao et al.   JOURNAL OF LIPID RESEARCH

Diffusion of GPI-anchored proteins is influenced by the activity of dynamic cortical actin

(2015) Suvrajit Saha et al.   MOLECULAR BIOLOGY OF THE CELL

Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases

(2015) Jannik Bruun Larsen et al.   Nature Chemical Biology

Dynamic label-free imaging of lipid nanodomains

(2015) Gabrielle de Wit et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sphingomyelin distribution in lipid rafts of artificial monolayer membranes visualized by Raman microscopy

(2015) Jun Ando et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Designing lipids for selective partitioning into liquid ordered membrane domains

(2015) Noor Momin et al.   Soft Matter

GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane

(2015) Eva Sevcsik et al.   Nature Communications

Adaptive Lipid Packing and Bioactivity in Membrane Domains

(2015) Erdinc Sezgin et al.   PLoS One

Growth Conditions and Cell Cycle Phase Modulate Phase Transition Temperatures in RBL-2H3 Derived Plasma Membrane Vesicles

(2015) Erin M. Gray et al.   PLoS One

The T cell receptor resides in ordered plasma membrane nanodomains that aggregate upon patching of the receptor

(2015) Jelena Dinic et al.   Scientific Reports

The cholesterol-binding motif of the HIV-1 glycoprotein gp41 regulates lateral sorting and oligomerization

(2014) Roland Schwarzer et al.   CELLULAR MICROBIOLOGY

Active organization of membrane constituents in living cells

(2014) Madan Rao et al.   CURRENT OPINION IN CELL BIOLOGY

Dehydroergosterol as an Analogue for Cholesterol: Why It Mimics Cholesterol So Well—or Does It?

(2014) Mohsen Pourmousa et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Lipid Organization of the Plasma Membrane

(2014) Helgi I. Ingólfsson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Measuring Lipid Packing of Model and Cellular Membranes with Environment Sensitive Probes

(2014) Erdinc Sezgin et al.   LANGMUIR

High-Speed Single-Particle Tracking of GM1 in Model Membranes Reveals Anomalous Diffusion due to Interleaflet Coupling and Molecular Pinning

(2014) Katelyn M. Spillane et al.   NANO LETTERS

Membrane proteins bind lipids selectively to modulate their structure and function

(2014) Arthur Laganowsky et al.   NATURE

RaftProt: mammalian lipid raft proteome database

(2014) Anup Shah et al.   NUCLEIC ACIDS RESEARCH

Tracking Cholesterol/Sphingomyelin-Rich Membrane Domains with the Ostreolysin A-mCherry Protein

(2014) Matej Skočaj et al.   PLoS One

Membrane raft association is a determinant of plasma membrane localization

(2014) B. B. Diaz-Rohrer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells

(2014) Alf Honigmann et al.   Nature Communications

A lipid bound actin meshwork organizes liquid phase separation in model membranes

(2014) Alf Honigmann et al.   eLife

Coarse-Graining Methods for Computational Biology

(2013) Marissa G. Saunders et al.   Annual Review of Biophysics

Laurdan Fluorescence Lifetime Discriminates Cholesterol Content from Changes in Fluidity in Living Cell Membranes

(2013) Ottavia Golfetto et al.   BIOPHYSICAL JOURNAL

Fluorescent Probes for Lipid Rafts: From Model Membranes to Living Cells

(2013) Andrey S. Klymchenko et al.   CHEMISTRY & BIOLOGY

Lypd6 Enhances Wnt/β-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains

(2013) Günes Özhan et al.   DEVELOPMENTAL CELL

Drug Uptake, Lipid Rafts, and Vesicle Trafficking Modulate Resistance to an Anticancer Lysophosphatidylcholine Analogue in Yeast

(2013) Álvaro Cuesta-Marbán et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Smoothened Oligomerization/Higher Order Clustering in Lipid Rafts Is Essential for High Hedgehog Activity Transduction

(2013) Dawei Shi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Sphingolipid Domains in the Plasma Membranes of Fibroblasts Are Not Enriched with Cholesterol

(2013) Jessica F. Frisz et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Bile Acids Modulate Signaling by Functional Perturbation of Plasma Membrane Domains

(2013) Yong Zhou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Direct imaging reveals stable, micrometer-scale lipid domains that segregate proteins in live cells

(2013) Alexandre Toulmay et al.   JOURNAL OF CELL BIOLOGY

Binding of a pleurotolysin ortholog fromPleurotus eryngiito sphingomyelin and cholesterol-rich membrane domains

(2013) Hema Balakrishna Bhat et al.   JOURNAL OF LIPID RESEARCH

Behavior of Fluorescent Cholesterol Analogues Dehydroergosterol and Cholestatrienol in Lipid Bilayers: A Molecular Dynamics Study

(2013) João R. Robalo et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Colocalization of the Ganglioside GM1 and Cholesterol Detected by Secondary Ion Mass Spectrometry

(2013) Mónica M. Lozano et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Hybrid and Nonhybrid Lipids Exert Common Effects on Membrane Raft Size and Morphology

(2013) Frederick A. Heberle et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Bilayer Thickness Mismatch Controls Domain Size in Model Membranes

(2013) Frederick A. Heberle et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The Molecular Structure of the Liquid-Ordered Phase of Lipid Bilayers

(2013) Alexander J. Sodt et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Uptake of oxLDL and IL-10 Production by Macrophages Requires PAFR and CD36 Recruitment into the Same Lipid Rafts

(2013) Francisco J. O. Rios et al.   PLoS One

Is the fluid mosaic (and the accompanying raft hypothesis) a suitable model to describe fundamental features of biological membranes? What may be missing?

(2013) Luis A. Bagatolli et al.   Frontiers in Plant Science

Biomolecular Simulation: A Computational Microscope for Molecular Biology

(2012) Ron O. Dror et al.   Annual Review of Biophysics

Partitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes

(2012) Erdinc Sezgin et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Active Remodeling of Cortical Actin Regulates Spatiotemporal Organization of Cell Surface Molecules

(2012) Kripa Gowrishankar et al.   CELL

Comparative lipidomics analysis of HIV-1 particles and their producer cell membrane in different cell lines

(2012) Maier Lorizate et al.   CELLULAR MICROBIOLOGY

To milliseconds and beyond: challenges in the simulation of protein folding

(2012) Thomas J Lane et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Palmitoylation of MPP1 (Membrane-palmitoylated Protein 1)/p55 Is Crucial for Lateral Membrane Organization in Erythroid Cells

(2012) Agnieszka Łach et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane

(2012) Mathias J. Gerl et al.   JOURNAL OF CELL BIOLOGY

Model membrane platforms to study protein-membrane interactions

(2012) Erdinc Sezgin et al.   MOLECULAR MEMBRANE BIOLOGY

Molecular recognition of a single sphingolipid species by a protein’s transmembrane domain

(2012) F.-Xabier Contreras et al.   NATURE

Elucidating membrane structure and protein behavior using giant plasma membrane vesicles

(2012) Erdinc Sezgin et al.   Nature Protocols

A critical survey of methods to detect plasma membrane rafts

(2012) E. Klotzsch et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Interferometric scattering microscopy (iSCAT): new frontiers in ultrafast and ultrasensitive optical microscopy

(2012) Jaime Ortega-Arroyo et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

A Mechanistic Model of Early FcεRI Signaling: Lipid Rafts and the Question of Protection from Dephosphorylation

(2012) Dipak Barua et al.   PLoS One

HIV-1 Gag protein can sense the cholesterol and acyl chain environment in model membranes

(2012) R. A. Dick et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo

(2012) S. A. Sanchez et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The role of lipids in defining membrane protein interactions: insights from mass spectrometry

(2012) Nelson P. Barrera et al.   TRENDS IN CELL BIOLOGY

Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution

(2012) Dylan M. Owen et al.   Nature Communications

STED Nanoscopy Reveals Molecular Details of Cholesterol- and Cytoskeleton-Modulated Lipid Interactions in Living Cells

(2011) V. Mueller et al.   BIOPHYSICAL JOURNAL

Minimal Model of Plasma Membrane Heterogeneity Requires Coupling Cortical Actin to Criticality

(2011) Benjamin B. Machta et al.   BIOPHYSICAL JOURNAL

Flying-Patch Patch-Clamp Study of G22E-MscL Mutant under High Hydrostatic Pressure

(2011) Evgeny Petrov et al.   BIOPHYSICAL JOURNAL

Methyl-β-Cyclodextrins Preferentially Remove Cholesterol from the Liquid Disordered Phase in Giant Unilamellar Vesicles

(2011) Susana A. Sanchez et al.   JOURNAL OF MEMBRANE BIOLOGY

Cholesterol modulates glycolipid conformation and receptor activity

(2011) Daniel Lingwood et al.   Nature Chemical Biology

Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis

(2011) Prabuddha Sengupta et al.   NATURE METHODS

Regulation of human EGF receptor by lipids

(2011) U. Coskun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Raft domains of variable properties and compositions in plasma membrane vesicles

(2011) I. Levental et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lateral sorting in model membranes by cholesterol-mediated hydrophobic matching

(2011) H.-J. Kaiser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular Simulation Approaches to Membrane Proteins

(2011) Phillip J. Stansfeld et al.   STRUCTURE

Lipid Simulations: A Perspective on Lipids in Action

(2011) I. Vattulainen et al.   Cold Spring Harbor Perspectives in Biology

Fluorescence Techniques to Study Lipid Dynamics

(2011) E. Sezgin et al.   Cold Spring Harbor Perspectives in Biology

Fluorescent probe partitioning in giant unilamellar vesicles of ‘lipid raft’ mixtures

(2010) Janos Juhasz et al.   BIOCHEMICAL JOURNAL

Greasing Their Way: Lipid Modifications Determine Protein Association with Membrane Rafts

(2010) Ilya Levental et al.   BIOCHEMISTRY

Limited cholesterol depletion causes aggregation of plasma membrane lipid rafts inducing T cell activation

(2010) Saleemulla Mahammad et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Comparison of Three Ternary Lipid Bilayer Mixtures: FRET and ESR Reveal Nanodomains

(2010) Frederick A. Heberle et al.   BIOPHYSICAL JOURNAL

Localization of uPAR and MMP-9 in lipid rafts is critical for migration, invasion and angiogenesis in human breast cancer cells

(2010) Hari Raghu et al.   BMC CANCER

Imaging of Mobile Long-lived Nanoplatforms in the Live Cell Plasma Membrane

(2010) Mario Brameshuber et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Palmitoylation regulates raft affinity for the majority of integral raft proteins

(2010) I. Levental et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lipid Rafts As a Membrane-Organizing Principle

(2010) D. Lingwood et al.   SCIENCE

Cholesterol-dependent phase separation in cell-derived giant plasma-membrane vesicles

(2009) Ilya Levental et al.   BIOCHEMICAL JOURNAL

FLIM-FRET and FRAP reveal association of influenza virus haemagglutinin with membrane rafts

(2009) Stephanie Engel et al.   BIOCHEMICAL JOURNAL

PI(4,5)P2 Degradation Promotes the Formation of Cytoskeleton-Free Model Membrane Systems

(2009) Heiko Keller et al.   CHEMPHYSCHEM

Hotspots of GPI-anchored proteins and integrin nanoclusters function as nucleation sites for cell adhesion

(2009) T. S. van Zanten et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Order of lipid phases in model and plasma membranes

(2009) H.-J. Kaiser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7

(2009) Simon Staubach et al.   PROTEOMICS

Critical Fluctuations in Plasma Membrane Vesicles

(2008) Sarah L. Veatch et al.   ACS Chemical Biology

Domain coupling in asymmetric lipid bilayers

(2008) Volker Kiessling et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Nanoclusters of GPI-Anchored Proteins Are Formed by Cortical Actin-Driven Activity

(2008) Debanjan Goswami et al.   CELL

Direct observation of the nanoscale dynamics of membrane lipids in a living cell

(2008) Christian Eggeling et al.   NATURE

Unilamellar vesicle formation and encapsulation by microfluidic jetting

(2008) J. C. Stachowiak et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plasma membranes are poised for activation of raft phase coalescence at physiological temperature

(2008) D. Lingwood et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein area occupancy at the center of the red blood cell membrane

(2008) A. D. Dupuy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA