期刊
NATURE METHODS
卷 14, 期 5, 页码 491-+出版社
NATURE PORTFOLIO
DOI: 10.1038/NMETH.4227
关键词
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资金
- NIH [AG017242, AG047200, AG038072]
- Glenn Foundation for Medical Research
Mutation analysis in single-cell genomes is prone to artifacts associated with cell lysis and whole-genome amplification. Here we addressed these issues by developing single-cell multiple displacement amplification (SCMDA) and a general-purpose single-cell-variant caller, SCcaller (https://github.com/biosinodx/SCcaller/). By comparing SCMDA-amplified single cells with unamplified clones from the same population, we validated the procedure as a firm foundation for standardized somatic-mutation analysis in single-cell genomics.
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