Continuous directed evolution of aminoacyl-tRNA synthetases

Nitrilase-Activatable Noncanonical Amino Acid Precursors for Cell-Selective Metabolic Labeling of Proteomes

(2016) Zefan Li et al.   ACS Chemical Biology

Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance

(2016) Ahmed H. Badran et al.   NATURE

Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids

(2015) Miriam Amiram et al.   NATURE BIOTECHNOLOGY

Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

(2015) Basil P Hubbard et al.   NATURE METHODS

Rationally evolving tRNAPylfor efficient incorporation of noncanonical amino acids

(2015) Chenguang Fan et al.   NUCLEIC ACIDS RESEARCH

Development of potent in vivo mutagenesis plasmids with broad mutational spectra

(2015) Ahmed H. Badran et al.   Nature Communications

Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

(2015) Joanne M. Ho et al.   ACS Synthetic Biology

Expanding and Reprogramming the Genetic Code of Cells and Animals

(2014) Jason W. Chin   Annual Review of Biochemistry

Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool

(2014) Wei Wan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

(2014) Wolfgang H. Schmied et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Negative selection and stringency modulation in phage-assisted continuous evolution

(2014) Jacob C Carlson et al.   Nature Chemical Biology

Polyspecific pyrrolysyl-tRNA synthetases from directed evolution

(2014) Li-Tao Guo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations

(2014) Bryan C. Dickinson et al.   Nature Communications

A Population-Based Experimental Model for Protein Evolution: Effects of Mutation Rate and Selection Stringency on Evolutionary Outcomes

(2013) Aaron M. Leconte et al.   BIOCHEMISTRY

Upgrading protein synthesis for synthetic biology

(2013) Patrick O'Donoghue et al.   Nature Chemical Biology

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

N-Acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possessN-acetyl lysine specificity in vitro and in vivo

(2012) Takuya Umehara et al.   FEBS LETTERS

PylSn and the Homologous N-terminal Domain of Pyrrolysyl-tRNA Synthetase Bind the tRNA That Is Essential for the Genetic Encoding of Pyrrolysine

(2012) Ruisheng Jiang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

An Asymmetric Synthesis of l-Pyrrolysine

(2012) Margaret L. Wong et al.   ORGANIC LETTERS

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Evolution of Amber Suppressor tRNAs for Efficient Bacterial Production of Proteins Containing Nonnatural Amino Acids

(2009) Jiantao Guo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli

(2009) Travis S. Young et al.   JOURNAL OF MOLECULAR BIOLOGY

A Method for Genetically Installing Site-Specific Acetylation in Recombinant Histones Defines the Effects of H3 K56 Acetylation

(2009) Heinz Neumann et al.   MOLECULAR CELL

Pyrrolysyl-tRNA synthetase–tRNAPyl structure reveals the molecular basis of orthogonality

(2009) Kayo Nozawa et al.   NATURE

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification

(2008) Tatsuo Yanagisawa et al.   CHEMISTRY & BIOLOGY

Crystallographic Studies on Multiple Conformational States of Active-site Loops in Pyrrolysyl-tRNA Synthetase

(2008) Tatsuo Yanagisawa et al.   JOURNAL OF MOLECULAR BIOLOGY

Genetically encoding Nε-acetyllysine in recombinant proteins

(2008) Heinz Neumann et al.   Nature Chemical Biology

New Molecular Reporters for Rapid Protein Folding Assays

(2008) Stéphanie Cabantous et al.   PLoS One

Immunochemical termination of self-tolerance

(2008) J. Grunewald et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA