Article
Immunology
Syong H. Nam-Cha, Elena Dominguez-Jurado, Selena L. Tinoco-Valencia, Ramon Perez-Tanoira, Noelia Morata-Moreno, Rocio Alfaro-Ruiza, Agustin Lara-Sanchez, Jaime Esteban, Rafael Lujan, Carlos Alonso-Moreno, Pedro Segui, Alberto Ocana, Angel Lopez Gonzalez, John J. Aguilera-Correa, Francisco C. Perez-Martinez, Milagros Molina Alarcon
Summary: This study aimed to translate clinical research outcomes based on metallodrugs into clinical practice for the treatment of MRSA. The novel metallodrug 2P-Pt showed antibacterial effect against MRSA and inhibited biofilm formation. These findings suggest that 2P-Pt may be a promising therapeutic alternative for MRSA infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Nikki N. Tran, Taylor Morrisette, Sarah C. J. Jorgensen, Jose M. Orench-Benvenutti, Razieh Kebriaei
Summary: Staphylococcus aureus is a significant cause of healthcare-associated and community-acquired infections, especially when associated with medical devices and in biofilm form. Biofilm structure allows for the enrichment of resistant and persistent phenotypes, leading to relapse and recurrence of infection. Minimal diffusion of antibiotics within biofilms leads to heterogeneity and distinct physiological activity. Horizontal gene transfer between cells in proximity adds to the challenges of eradicating biofilms. This review focuses on biofilm-associated infections caused by S. aureus, the impact of environmental conditions on biofilm formation, interactions within biofilm communities, and the clinical challenges they pose. Potential solutions, novel treatment strategies, combination therapies, and reported alternatives are also discussed.
Article
Biotechnology & Applied Microbiology
Georgios Katsipis, Anastasia A. Pantazaki
Summary: In this study, the antimicrobial effects of SPT on Staphylococcus aureus biofilms were demonstrated. SPT reduced the content of cell wall components, altered the activity of alkaline phosphatases and proteins involved in phosphate metabolism, and weakened the resistance of bacteria to biofilm formation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Hui-Hui Yeo, Chung-Wei Shiau, Yu-Hsuan Jao, Tsai-Wen Wan, Hao-Chieh Chiu
Summary: This study found that oleic acid enhances the activity of the antibacterial agent SC5005 against Staphylococcus aureus and MRSA strains. Specifically, cis-form unsaturated fatty acids, such as docosahexaenoic acid (DHA), synergistically increase the potency of SC5005. The combination of SC5005 and DHA can effectively eradicate MRSA planktonic and persister cells, as well as biofilms.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Salman Jameel, Sadaqat Farooq, Ifshana Gani, Syed Riyaz-Ul-Hassan, Khursheed Ahmad Bhat
Summary: SAJ-1, SAJ-2, and SB-11 showed strong antibacterial properties against Staphylococcus aureus and may be potential chemical entities for the development of antibacterial substances. SAJ-1 and SAJ-2 are the most suitable sclareol analogues for further studies towards the development of antibacterial substances. More research utilizing in vivo models should be conducted to evaluate the efficacy of these compounds as antibiotics.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Jelle Vlaeminck, Qiang Lin, Basil Britto Xavier, Sarah De Backer, Matilda Berkell, Henri De Greve, Jean-Pierre Hernalsteens, Samir Kumar-Singh, Herman Goossens, Surbhi Malhotra-Kumar
Summary: There are striking differences in gene expression during biofilm formation by three important pandemic MRSA clones, USA300, HEMRSA-15, and ST239. The clfA gene is the only significantly upregulated gene across all three strains in 24-hour biofilm cultures and represents an important target for disrupting early biofilms. Furthermore, our data indicate the critical role of arginine catabolism pathways in early biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Jing Wang, Zhicun Sheng, Yunying Liu, Xiaolan Chen, Shuaibing Wang, Haifeng Yang
Summary: This study analyzed the proteome and transcriptome of Staphylococcus aureus and found that tannic acid (TA) has an inhibitory effect on this pathogen. TA damaged the integrity of the cell membrane and affected the expression and abundance of key enzymes, leading to impaired growth and synthesis in S. aureus. These findings provide a theoretical basis for the application of TA as an antibacterial chemotherapeutic agent.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Carmine Buonocore, Rosa Giugliano, Gerardo Della Sala, Fortunato Palma Esposito, Pietro Tedesco, Veronica Folliero, Massimiliano Galdiero, Gianluigi Franci, Donatella de Pascale
Summary: In this study, the antimicrobial activity of a rhamnolipids mixture produced by the Antarctic marine bacterium Pseudomonas gessardii M15 against multidrug-resistant Staphylococcus aureus was evaluated. The mixture demonstrated bactericidal activity, penetration of biofilm, and prevention of bacterial proliferation on wound dressings.
Article
Microbiology
Francesca Sivori, Ilaria Cavallo, Daniela Kovacs, Maria Guembe, Isabella Sperduti, Mauro Truglio, Martina Pasqua, Grazia Prignano, Arianna Mastrofrancesco, Luigi Toma, Fulvia Pimpinelli, Aldo Morrone, Fabrizio Ensoli, Enea Gino Di Domenico
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) has become the leading cause of skin and soft tissue infections (SSTIs) and biofilm formation contributes to increased antibiotic tolerance. This study demonstrates that dalbavancin is the most effective antibiotic against MRSA biofilms and extracellular DNA affects its activity. The findings provide new insights for developing targeted therapeutic strategies against MRSA.
MICROBIOLOGY SPECTRUM
(2022)
Article
Dermatology
Torstein Gronseth, Kirill Ovchinnikov, Harald Carlsen, Jurate Saltyte Benth, Dzung B. Diep, Magnus von Unge, Juha T. Silvola
Summary: This study evaluated the antibacterial efficacy of Lugol's solution and Gentian violet against MRSA biofilm in vivo, and found that these two agents effectively eradicated MRSA biofilm, suggesting their potential as alternatives or adjuncts to topical antibiotics.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Infectious Diseases
Katarzyna Kosznik-Kwasnicka, Natalia Kazmierczak, Lidia Piechowicz
Summary: Biofilms are complex structures composed of bacterial cells embedded in extracellular polymeric substances (EPS). They are difficult to eradicate and bacteriophages have shown to penetrate the matrix and reach the bacterial cells. However, biofilm regrowth and phage-resistant bacteria are concerns. The combination of staphylococcal phages and lactoferrin, a protein with anti-biofilm properties, accelerates the anti-biofilm effect and prevents biofilm re-growth, showing potential use in medical settings for biofilm eradication procedures.
Article
Microbiology
Karina M. S. Herrera, Gabriela F. M. Lopes, Michael E. Oliveira, Jaqueline F. Sousa, William G. Lima, Fernanda K. Silva, Julio C. M. Brito, Ana Julia P. S. Gomes, Gustavo H. R. Viana, Adriana C. Soares, Jaqueline M. S. Ferreira
Summary: This study evaluated the antibacterial activity of five 3-alkyl-pyridinic analogs against MRSA. Compound 6 showed promising antibacterial activity, exhibited a rapid bactericidal action, and had low resistance induction potential. It also showed an additive effect when combined with other antibiotics.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Aida Hamdan-Partida, Samuel Gonzalez-Garcia, Francisco Javier Martinez-Ruiz, Miguel Angel Zavala-Sanchez, Anaid Bustos-Hamdan, Jaime Bustos-Martinez
Summary: The aim of this study was to determine the source of contamination of mobile phones and characterize the corresponding genotypic and phenotypic properties of the isolated strains of Staphylococcus aureus. The study found that the strains of S. aureus present in the nose and/or pharynx of phone owners can contaminate mobile phones, and these strains can have different genotypes.
Article
Immunology
John Jairo Aguilera-Correa, Liisa Nohynek, Hanna-Leena Alakomi, Jaime Esteban, Kirsi-Marja Oksman-Caldentey, Riitta Puupponen-Pimiae, Teemu J. Kinnari, Ramon Perez-Tanoira
Summary: This study aimed to evaluate the effect of two arctic berry extracts on MRSA biofilm development and treatment. The results showed that all berry extracts except for the raspberry press cake hydrothermal extract prevented biofilm development of three MRSA strains.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Shuang Wang, Xi Huang, Jie Yang, Dan Yang, Yue Zhang, Yanyan Hou, Lin Lin, Lin Hua, Wan Liang, Bin Wu, Zhong Peng
Summary: In this study, three phages were found to have the ability to kill most MRSA strains, and one of them was mutated into a highly virulent phage. Experimental results showed that this mutated phage had better capability in eliminating biofilms formed by MRSA strains. Mouse experiments demonstrated that injection of this phage was safe to the mice and treatment with it inhibited the development of abscess induced by MRSA and promoted the recovery from the clinical signs.
MICROBIOLOGICAL RESEARCH
(2023)
