期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 14, 期 7, 页码 2375-2385出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2017.06.002
关键词
Magnetic responsive hydrogels; Functionalized SPIONs; Platelet lysate; Co-culture system; Tendon-to-bone interface
资金
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/96593/2013, SFRH/BPD/112459/2015, SFRH/BPD/111729/2015, IF/00685/2012]
- FCT/MCTES (Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia, Tecnologia, e Ensino Superior)
- Fundo Social Europeu through Programa Operacional do Capital Humano (FSE/POCH) [PD/59/2013, PD/BD/113807/2015]
- ON.2 (NSRF) through ERD [RL3-TECT-NORTE-07-0124-FEDER-000020]
- Recognize project [UTAP-ICDT/CTM-BIO/0023/2014]
- Fundação para a Ciência e a Tecnologia [UTAP-ICDT/CTM-BIO/0023/2014, PD/BD/113807/2015] Funding Source: FCT
Photocrosslinkable magnetic hydrogels are attracting great interest for tissue engineering strategies due to their versatility and multifunctionality, including their remote controllability ex vivo, thus enabling engineering complex tissue interfaces. This study reports the development of a photocrosslinkable magnetic responsive hydrogel made of methacrylated chondroitin sulfate (MA-CS) enriched with platelet lysate (PL) with tunable features, envisioning their application in tendon-to-bone interface. MA-CS coated iron-based magnetic nanoparticles were incorporated to provide magnetic responsiveness to the hydrogel. Osteogenically differentiated adipose-derived stem cells and/or tendon-derived cells were encapsulated within the hydrogel, proliferating and expressing bone-and tendon-related markers. External magnetic field (EMF) application modulated the swelling, degradation and release of PL-derived growth factors, and impacted both cell morphology and the expression and synthesis of tendon-and bone-like matrix with a more evident effect in co-cultures. Overall, the developed magnetic responsive hydrogel represents a potential cell carrier system for interfacial tissue engineering with EMF-controlled properties. (c) 2017 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据