Article
Biochemical Research Methods
Yi Cen, Zhongping Wang, Peixiong Ke, Wenting Zhu, Zhongwen Yuan, Senling Feng, Yiqing Chen, Caiyan Lin, Xiaomin Liu, Yuting Li, Pengke Yan
Summary: This study demonstrates that aptamers Apt3 and Apt6 have high affinity and strong specificity, and the dual-aptamer sandwich ELONA method has a wide detection range, making it suitable for determining cTnI in serum with potential applications in AMI diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Brady Layman, Brian Mandella, Jessica Carter, Haley Breen, John Rinehart, Anna Cavinato
Summary: Bacterial kidney disease is a major health problem for salmonids caused by Renibacterium salmoninarum. This study isolated and characterized an aptamer with high affinity to the principal antigen found on the cell wall surface of Rs, which could potentially be used for rapid detection and diagnosis in the future.
Article
Medical Laboratory Technology
Xian-hui Zhang, Wei Wang, Xin Chen
Summary: This study aimed to identify aptamers binding specifically to acute promyelocytic leukemia (APL) NB4 cells, with the aptamer CX9 showing the highest affinity with a dissociation constant of 16.2 nM. The study laid groundwork for rapid diagnostic methods using fluorescence-labeled aptamers to detect APL.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Biochemical Research Methods
Abinaya Jaisankar, Sasirekha Krishnan, Loganathan Rangasamy
Summary: This article gives a brief overview of aptamer-based lateral flow assay (ALFA) in biomedical applications, including its principles, formats, aptamer selection process, and critical components for signal amplification. ALFA is considered to be superior to antibody-based LFA because of its higher sensitivity and specificity, as well as its portability, rapid detection, and simple visual output.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Engineering, Biomedical
Shuming Sun, Han Liu, Yan Hu, Yanpeng Wang, Mingri Zhao, Yijun Yuan, Yafei Han, Yingying Jing, Jin Cui, Xiaoxiang Ren, Xiao Chen, Jiacan Su
Summary: In this study, human skeletal muscle-specific ssDNA aptamers were selected and identified using SELEX. The aptamers showed preferential interaction with human skeletal muscle cells in vitro and specifically targeted these cells in vivo. The formation of a disulfide bond with nanoliposomes did not affect the ability of the aptamers to target skeletal muscle cells. This newly identified ssDNA aptamer and nanoliposome modification have potential applications in the treatment of human skeletal muscle diseases.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Xiaomin Zhang, Ge Yang, Wenjing Liu, Qing Liu, Zhuoran Wang, Kelong Fan, Feng Qu, Yuanyu Huang
Summary: Using CE-SELEX, four candidate aptamers with a stem-loop structure were selected targeting LRP6 protein. Among them, AptLRP6-A3 showed the highest affinity with LRP6-overexpressed human breast cancer cells. This LRP6 aptamer holds promise for rapid diagnosis and treatment of LRP6-related diseases.
Review
Chemistry, Analytical
Marimuthu Citartan
Summary: Dot blot assays have traditionally used antibodies as the primary molecular recognition element, but now aptamers can also be utilized. These assays are often used to monitor SELEX progress and assess the potency of aptamer candidates. Efforts to expand aptamer-based dot blot assays for diagnostic purposes are currently slow, suggesting a need for further development.
Article
Biochemistry & Molecular Biology
Xiaomin Zhang, Ge Yang, Yi Zhao, Xuyan Dai, Wenjing Liu, Feng Qu, Yuanyu Huang
Summary: As a transmembrane serine protease, fibroblast activation protein (FAP) is expressed on the surface of fibroblasts associated with various epithelial-derived malignancies. It plays a role in tumorigenesis, progression, and immunosuppression. This study selected five candidate aptamers for FAP using capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX), and determined their secondary structures and affinity to FAP protein through CE-laser-induced fluorescence (LIF) method. The FAP aptamers, especially AptFAP-A4, showed potential for rapid tumor diagnosis and targeted therapy.
Review
Biochemical Research Methods
Ming-Qing Tang, Jing Xie, Liang-Ming Rao, Ya-Jie Kan, Pei Luo, Lin-Sen Qing
Summary: CRP, a non-specific acute-phase indicator of inflammation, is widely recognized in clinical diagnostic applications. Aptamer-based biosensors are the focus of research for high-sensitivity analysis of CRP, with progress in in vitro aptamer screening and recent advances in aptamer-based CRP sensor applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
S. S. Narlawar, S. Gandhi
Summary: Graphene oxide and graphene nanoplatelets were compared for their efficiency in the partitioning process, with graphene nanoplatelets showing better performance. A partitioning cartridge was successfully fabricated using spin columns from molecular biology lab waste, demonstrating efficient separation of target bound ssDNA.
MATERIALS TODAY ADVANCES
(2021)
Article
Food Science & Technology
Miaojia Xie, Zanlin Chen, Fengguang Zhao, Ying Lin, Suiping Zheng, Shuangyan Han
Summary: In this study, three DNA aptamers with good affinity for malachite green were obtained by Capture-SELEX. These aptamers can sensitively and specifically detect malachite green in a FRET fluorescent aptasensor, showing high specificity and recovery rate.
Article
Microbiology
Qing Yu, Mengmeng Li, Mingzhu Liu, Shuaishuai Huang, Gaoxue Wang, Taixia Wang, Pengfei Li
Summary: The study generated three DNA aptamers (LBVA1, LBVA2, and LBVA3) against LMBV-infected fathead minnow cells through SELEX technology. These aptamers exhibited efficient antiviral activities in vitro, indicating their potential in developing effective anti-viral treatments.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hui Zhang, Xue Li, Ailan Huang, Zhifeng Yan, Yang Chen, Zijun Bie
Summary: This study developed a facile and efficient SELEX approach for in vitro evolution of saponin-binding aptamers, allowing for selective enrichment of target saponins from complex biological samples. The resulting aptamers enabled quantitative analysis of ginsenoside Re in real samples with high reliability, demonstrating a promising approach for aptamer-based research and pharmaceutical analysis.
Article
Chemistry, Analytical
Yanping He, Jingrui Zhang, Ruifen Tian, Yuying Lu, Ling Pan, Yuzhong Zhang
Summary: The abuse of sulfameter (SME) in animal husbandry can lead to drug resistance and toxic or allergic reactions in humans. Therefore, it is crucial to develop a simple, cost-effective, and efficient method for detecting SME in food. In this study, a single fluorescent aptamer/graphene oxide (GO)-based biosensor was proposed for detecting SME residues in milk. The biosensor demonstrated high sensitivity, a wide linear range, and accurate detection of SME residues in milk samples.
Review
Chemistry, Multidisciplinary
Haixiang Yu, Obtin Alkhamis, Juan Canoura, Yingzhu Liu, Yi Xiao
Summary: This review discusses the challenges and advances in isolating and characterizing small-molecule-binding DNA aptamers, including in vitro methodologies for isolation, strategies for optimal binding properties, techniques for characterizing aptamer-target binding and structure, and various small-molecule sensing platforms and detection applications based on aptamers.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemistry & Molecular Biology
Michelle Bauer, Mia Strom, David S. Hammond, Sarah Shigdar
Article
Biochemistry & Molecular Biology
Joanna Macdonald, Delphine Denoyer, Justin Henri, Adelaide Jamieson, Ingrid J. G. Burvenich, Normand Pouliot, Sarah Shigdar
NUCLEIC ACID THERAPEUTICS
(2020)
Article
Medicine, Research & Experimental
Tao Wang, Kamal Rahimizadeh, Rakesh N. Veedu
MOLECULAR THERAPY-NUCLEIC ACIDS
(2020)
Review
Biochemistry & Molecular Biology
Poshmaal Dhar, Rasika M. Samarasinghe, Sarah Shigdar
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Genetics & Heredity
Madhuri Chakravarthy, Suxiang Chen, Tao Wang, Rakesh N. Veedu
Review
Biochemistry & Molecular Biology
Bakhtiar Bukari, Rasika M. Samarasinghe, Jinjutha Noibanchong, Sarah L. Shigdar
Review
Biochemistry & Molecular Biology
Mia Strom, Tamsyn Crowley, Sarah Shigdar
Summary: Hospital-acquired infections (HAIs) are a growing concern globally, with bacteria being the most common cause and highly resistant to antibiotics. Detecting and eradicating bacteria in the environment can reduce infection rates effectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Correction
Multidisciplinary Sciences
Hadi AlShamaileh, Tao Wang, Dongxi Xiang, Wang Yin, Phuong Ha-Lien Tran, Roberto A. Barrero, Pei-Zhuo Zhang, Yong Li, Lingxue Kong, Ke Liu, Shu-Feng Zhou, Yingchun Hou, Sarah Shigdar, Wei Duan
SCIENTIFIC REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Chris Vi, Giovanni Mandarano, Sarah Shigdar
Summary: Breast cancer, a common cancer in women, exhibits high heterogeneity. Overexpression of HER2 is strongly associated with poor outcomes of breast cancer. Molecular imaging offers sensitive detection for early diagnosis, crucial for improving prognosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Sarah Shigdar, Brett Schrand, Paloma H. Giangrande, Vittorio de Franciscis
Summary: The development of aptamer-based therapeutics has rapidly progressed, with eleven aptamers entering clinical trials for various therapeutic indications. Despite some success in pre-clinical and clinical studies, no aptamer has been FDA-approved for cancer treatment. Recent research focuses on enhancing anti-cancer immunity, targeting hard-to-treat cancers, and developing carrier aptamers for delivering RNA therapeutics.
Review
Pharmacology & Pharmacy
Maryam Nakhjavani, Rasika M. Samarasinghe, Sarah Shigdar
Summary: The key challenges in treating triple-negative breast cancer brain metastasis (TNBC-BM) lie in the absence of targeted therapy and difficulties in delivering drugs to the brain. This review introduces current drugs based on their molecular targets and seeks to improve drug delivery to the brain using more efficient and promising drug delivery systems. Ongoing clinical trials on druggable targets in TNBC-BM are described to achieve more targeted treatment, along with an introduction to the obstacles hindering drug delivery to the brain and strategies for future advancements in TNBC-BM patient treatment.
DRUG DISCOVERY TODAY
(2022)
Article
Pharmacology & Pharmacy
Maryam Nakhjavani, Sarah Shigdar
Summary: Triple-negative breast cancer (TNBC) has the worst prognosis among breast cancer subtypes and lacks targeted therapy. Immunotherapy targeting PD-1/PD-L1 has shown promise. Aptamers, with their better tissue penetration and lower production cost compared to mAbs, present a potential pathway for PD-1/PD-L1 modulation in the future.
PHARMACOLOGICAL RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Sarah Shigdar, Lisa Agnello, Monica Fedele, Simona Camorani, Laura Cerchia
Summary: The identification of tumor cell-specific surface markers is crucial for personalized cancer medicine. Currently, the repertoire of approved treatments targeting cell membrane is limited, resulting in the inability to treat many cancers. Cell-SELEX technology provides a valid complement to existing strategies for biomarkers' discovery and has the potential to increase the number of cancer cell-surface biomarkers.
Article
Pharmacology & Pharmacy
Mohsen Sarafraz, Maryam Nakhjavani, Sarah Shigdar, Farid C. Christo, Bernard Rolfe
Summary: The blood-brain barrier is a major obstacle in brain cancer treatment, but targeting transferrin receptors using aptamers can increase drug delivery. The thickness of the astrocyte and endothelial cell layers is identified as a key parameter affecting drug concentration. The drug delivery mechanism switches from convective mass transport to mixed convection mass transport and finally to diffusion as it reaches the brain parenchyma.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Review
Chemistry, Medicinal
Umair Khalid, Chris Vi, Justin Henri, Joanna Macdonald, Peter Eu, Giovanni Mandarano, Sarah Shigdar
