Article
Ophthalmology
Ming Chen, Lily Kim, Carolyn W. Lu, Hong Zeng, Douglas Vollrath
Summary: The study successfully generated a transgenic mouse line with consistently efficient RPE-selective Cre activity that could be temporally regulated using phi C31 integrase. This line provides a useful tool for temporally regulated modulation of gene function in the murine RPE.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Rahul Kumar, Yun Mao, Sonika Patial, Yogesh Saini
Summary: This study investigated the effect of different Tamoxifen (TAM) regimens on whole-body gene deletion in mice using Tamoxifen-inducible Cre recombinase. The results demonstrated that a combination of TAM-diet with either TAM-injections or TAM-oral gavage showed the most efficient deletion of genes in the whole body.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Martina Rossi, Aude Salomon, Nicolas Chaumontel, Jenny Molet, Sabine Bailly, Emmanuelle Tillet, Claire Bouvard
Summary: The Cre-lox system is a powerful tool for manipulating genes in mice, allowing for precise control of gene deletion. However, our study shows that tamoxifen activation of CreERT2 in young mice can result in severe hematological defects and mortality, even in the absence of a specific gene target. We emphasize the importance of including appropriate controls and improving communication about the limitations of Cre-lox mouse models among researchers.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Laura Pilar Aguado-Alvaro, Nerea Garitano, Gloria Abizanda, Eduardo Larequi, Felipe Prosper, Beatriz Pelacho
Summary: This study compares the efficiency and specificity of three commonly used fibroblast reporter transgenic mouse models for studying cardiac fibrosis. The results suggest that the PDGFRa-creERT2 mouse strain is the optimal model for CF tracking, while the Col1a1-CreERT2 mouse strain is not suitable. The PostnMCM line is the most reliable model for future cardiovascular disease studies, with specific fluorescent reporter expression in activated CF but not in ECs.
Article
Developmental Biology
Henrik Ortsater, Magda N. Hernandez-Vasquez, Maria H. Ulvmar, Alexander Gow, Taija Makinen
Summary: The generation of a transgenic mouse expressing tamoxifen inducible CreER(T2) under the control of Cldn11 promoter allows for selective and temporally controlled targeting of lymphatic valve endothelial cells. The study shows that CLDN11 is specifically expressed in lymphatic valves and not required for their development, with efficient labeling of valve endothelial cells observed. This mouse model provides a valuable tool for functional studies of valves.
Article
Oncology
Morsheda Nasrin, Osama Ahmed, Xujun Han, Md Nojebuzzaman, Ahmed Abo-Ahmed, Shigenobu Yazawa, Masatake Osawa
Summary: Conditional and inducible gene targeting using Cre/loxP-mediated recombination was achieved in melanocyte lineage by establishing the Pmel(P2A-TetON3G-TRE3G-iCre) mouse line, allowing temporal gene manipulation for studying gene functions. The efficiency of this mouse line was demonstrated by inducing Notch signaling ablation, resulting in premature hair graying. Additionally, unexpected PMEL-expressing cells were identified in non-pigmentary organs, suggesting unanticipated functions of PMEL.
PIGMENT CELL & MELANOMA RESEARCH
(2023)
Article
Biology
Helene Duplus-Bottin, Martin Spichty, Gerard Triqueneaux, Christophe Place, Philippe Emmanuel Mangeot, Theophile Ohlmann, Franck Vittoz, Gael Yvert
Summary: LiCre is a novel light-inducible Cre recombinase, capable of being activated within minutes by blue light with faster and stronger activation effects and lower residual activity in the dark. It shows high efficiency in both yeast and human cells, making it suitable for fundamental research, biomedical research, and industrial bioprocess control.
Article
Cell Biology
Yi-Chao Hsu, Yu-Fen Chung, Mei-Shu Chen, Chi-Kuang Wang, Si-Tse Jiang, Ing-Ming Chiu
Summary: The F1A-CreER(T2) mouse line, driven by the Fgf1A promoter, can be used for time-dependent and lineage tracing of Fgf1A-expressing cells in vivo.
Article
Biology
Katherine A. Kiwimagi, Justin H. Letendre, Benjamin H. Weinberg, Junmin Wang, Mingzhe Chen, Leandro Watanabe, Chris J. Myers, Jacob Beal, Wilson W. Wong, Ron Weiss
Summary: Kiwimagi & Letendre et al. present a workflow to quantitatively define recombinase-based digitizer and predict responses to different input signals. With a mechanistic/phenotypic model that can predict circuit performance, they generate a synthetic cell-cell communication device that amplifies a synNotch output signal.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell Biology
Luming Guo, Xiaoling Xie, Jing Wang, Haiyan Xiao, Shuchun Li, Mei Xu, Ebenezer Quainoo, Rithwik Koppaka, Jiaping Zhuo, Sylvia B. Smith, Lin Gan
Summary: The generation and characterization of a knock-in mouse line, Rbpms-CreER(T2), that allows for the conditional activation of a reporter gene in retinal ganglion cells (RGCs) is reported. The knock-in mice showed efficient recombination and no detectable defects, making them a powerful tool for studying RGCs.
Review
Biochemistry & Molecular Biology
Megan N. Michalski, Bart O. Williams
Summary: This review discusses the development and application of genetically engineered mouse models in musculoskeletal biology research, with a particular focus on tissue-specific gene knockout and the impact of CRISPR/Cas technology.
Article
Endocrinology & Metabolism
Xiao Wang, Fanyi Qiu, Junjie Yu, Meiyang Zhou, Anjian Zuo, Xiaojiang Xu, Xiao-Yang Sun, Zhengpin Wang
Summary: Using physical microdissection, researchers isolated the initial segment (IS) and proximal caput (P-caput) of the mouse epididymis and identified region-specific genes through RNA sequencing. These genes were found to be associated with sperm transport, maturation, and male fertility, making them potential targets for male contraception and providing insights into the epididymal microenvironment.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Shiting Liang, Youliang Wang, Meixia Kang, Juan Deng, Liting Chen, Xizhen Hong, Fan Fan Hou, Fujian Zhang
Summary: Protein reabsorption in renal proximal tubules is crucial for maintaining nutrient balance. We developed a new mouse model, AMN (CreERT2) knock-in mice, which express a fusion protein of Cre recombinase and estrogen receptor under the control of the AMN gene promoter specifically in renal proximal tubules. This model allows for the conditional knockout of genes in renal proximal tubules, providing valuable insights into their physiological function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alexander A. Dolskiy, Andrey S. Gudymo, Oleg S. Taranov, Irina V. Grishchenko, Ekaterina M. Shitik, Dmitry Yu Prokopov, Vladislav O. Soldatov, Elvira V. Sobolevskaya, Sergey A. Bodnev, Natalia V. Danilchenko, Anastasia A. Moiseeva, Polina Y. Torzhkova, Yulia A. Bulanovich, Galina S. Onhonova, Elena K. Ivleva, Marina V. Kubekina, Andrey E. Belykh, Tatiana V. Tregubchak, Alexander B. Ryzhikov, Elena V. Gavrilova, Rinat A. Maksyutov, Alexey V. Deykin, Dmitry V. Yudkin
Summary: COVID-19 has become a socially significant infection and the researchers have developed a new transgenic mouse model with human ACE2 gene expression for studying SARS-CoV-2 infection. The study found that the virus had the highest viral load in the lungs, brain, heart, and intestine. This model provides a useful tool for COVID-19 research.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Vinaya Sahasrabuddhe, Hiyaa Singhee Ghosh
Summary: This study investigates the potential detrimental effects of Cx3cr1-Cre activity on microglia and reveals an age-dependent vulnerability of microglia to Cre induction. Early postnatal microglia are more sensitive to Cre activity, while adult microglia do not show these detrimental effects.
