Article
Biochemistry & Molecular Biology
Andrew J. Nickerson, Vazhaikkurichi M. Rajendran
Summary: The corticosteroid hormone aldosterone enhances K+ secretion in the colon and plays a critical role in maintaining K+ balance. Previous studies showed that aldosterone up-regulates basolateral NKCC1 specifically in the distal colon to support K+ secretion, but its effects on the proximal colon are unclear. Understanding these region-specific effects is important for managing gastrointestinal pathologies.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Andrew J. Nickerson, Vazhaikkurichi M. Rajendran
Summary: Recent studies have shown that flupirtine stimulates NHE-3-dependent Na+ absorption in the colon while inhibiting Cl- secretion, likely due to reduced stimulatory input to the epithelium by submucosal enteric nervous system neurons. This novel mechanism could be beneficial for developing new treatments for diarrheal disorders such as IBS-D.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Article
Food Science & Technology
Marta Mendel, Wojciech Karlik, Urszula Latek, Magdalena Chlopecka, Ewelina Nowacka-Kozak, Katarzyna Pietruszka, Piotr Jedziniak
Summary: The study evaluated the effects of deoxynivalenol (DON) on the absorption of the commonly used antibiotics amoxicillin (AMX) and doxycycline (DOX) in pigs. Results showed that DON increased intestinal permeability, accelerated DOX transport, but did not affect AMX transport.
Article
Pharmacology & Pharmacy
Henrique Rodrigues Marcelino, Audrey Solgadi, Monique Cheron, Eryvaldo Socrates Tabosa do Egito, Gilles Ponchel
Summary: This study aimed to explore the effects of different aggregation states of AmB in nanocarriers and their influence on jejunal permeability. Physicochemical characterization revealed different degrees of AmB aggregation states. However, although this approach had an impact on the toxicity of AmB, its effect on permeability was not significant enough.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Marine & Freshwater Biology
Marine Pyl, Angus Taylor, Francois Oberhansli, Peter Swarzenski, Leila Hussamy, Marc Besson, Bruno Danis, Marc Metian
Summary: The present study demonstrates the ability of marine microplastics to cross the intestinal wall of sea urchins and enter the coelomic fluid. The size-dependence of microplastic translocation across the intestinal wall is also highlighted.
AQUATIC TOXICOLOGY
(2022)
Article
Pharmacology & Pharmacy
Fiona McCartney, Diego R. Perinelli, Mattia Tiboni, Robert Cavanagh, Simone Lucarini, Giovanni Filippo Palmieri, Luca Casettari, David J. Brayden
Summary: The study compared the performance of three monoesters with varying sugar moieties as intestinal permeation enhancers. Results showed that all three esters enhanced permeability in rat intestinal tissue likely via tight junction modulation, while C12-trehalose was slightly less efficacious but also less damaging to the tissue. All three esters have potential for oral formulations as permeation enhancers.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Food Science & Technology
Ng'Andwe Kalungwana, Lisa Marshall, Alan Mackie, Christine Boesch
Summary: To accurately assess the absorption of nutrients in food, it is important to simulate human digestion using model settings. This study compared the uptake and transportation of dietary carotenoids using two different models. The results showed that the mouse intestinal tissue had a higher uptake and absorption efficiency than the Caco-2 cells. These findings demonstrate the usefulness of physiologically relevant models for studying human intestinal absorption.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Agriculture, Dairy & Animal Science
Marco Tretola, Paolo Silacci, Raquel Sousa, Francesca Colombo, Sara Panseri, Matteo Ottoboni, Luciano Pinotti, Giuseppe Bee
Summary: Polyphenols interact with protein fractions in feed ingredients, affecting digestibility. This study found that tannin-rich chestnut extract decreased the digestibility of soy-based feeds and demonstrated protective effects on intestinal epithelium in vitro.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Joelle Houriet, Yvonne E. Arnold, Leonie Pellissier, Yogeshvar N. Kalia, Jean-Luc Wolfender
Summary: The study used liquid chromatography-mass spectrometry to monitor the absorption and metabolism of an extract of Pueraria lobata rich in isoflavones in viable porcine small intestine ex vivo, revealing stability and absorption patterns of different types of isoflavones. This approach shows potential for HP research without the need for in vivo studies.
Review
Pharmacology & Pharmacy
Kazuya Maeda
Summary: The prediction of intestinal absorption of drugs is crucial in the development of oral drugs, but is challenging due to various factors influencing drug absorption and the differences between animal and human bioavailability. Traditionally, Caco-2 cell transcellular transport assay has been used for screening drug absorption properties, but its predictability is limited. Recently, novel in vitro experimental systems, such as human-derived intestinal samples and differentiated epithelial cells from intestinal stem cells, have been proposed. Crypt-derived differentiated epithelial cells show advantages in characterizing species and regional differences in drug absorption.
DRUG METABOLISM AND DISPOSITION
(2023)
Article
Agriculture, Dairy & Animal Science
Daniel H. M. Watanabe, John Doelman, Michael A. Steele, Le L. Guan, Dave J. Seymour, Gregory B. Penner
Summary: This study evaluated the effects of Ca-gluconate relative to butyrate on gastrointestinal tract function in beef heifers. The results showed that only Ca-butyrate was able to stimulate ruminal short-chain fatty acid absorption in growing beef heifers.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Veterinary Sciences
Franziska Dengler, Harald M. Hammon, Wendy Liermann, Solvig Goers, Lisa Bachmann, Christiane Helm, Reiner Ulrich, Cora Delling
Summary: Cryptosporidiosis, a common cause of diarrhea in infants and young livestock, has a potential impact on glucose metabolism in neonatal calves infected with C. parvum. The study showed that despite an increased transepithelial transport of glucose, infected calves experienced decreased plasma glucose concentration and oral glucose absorption. However, no differences in the abundance of glucose transporters were observed in infected calves, suggesting that the upregulation of uptake mechanisms and metabolic machinery in host cells compensate for the energy losses caused by the parasite.
VETERINARY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Kenichi Kaneko, Ryosuke Miyasaka, Roslyn Hayman
Summary: Coating poorly water-soluble drugs with nano-hydroxyapatite (nano-HAP) has been shown to improve their bioavailability after oral administration. In this study, we coated acetazolamide (AZ), a BCS Class IV drug, with nano-HAP and investigated its bioavailability and the role of nano-HAP in promoting it. Our results suggest that nano-HAP enhances drug permeability from the small intestine through intracellular transport via the intestinal villi, rather than tight junction penetration.
Article
Pharmacology & Pharmacy
Hyeong Min Kim, Hyounkoo Han, Hye Kyoung Hong, Ji Hyun Park, Kyu Hyung Park, Hyuncheol Kim, Se Joon Woo
Summary: The permeability of ocular drugs in the retinal-RPE-choroid-sclera and RPE-choroid-sclera layers is influenced by the molecular weight and lipophilicity, affecting their intravitreal half-life. Lipophilic brimonidine showed better permeability in the RCS and CS layers, while ganciclovir had better permeability in the neural retina. Bevacizumab, with a larger molecular weight, demonstrated lower permeability compared to brimonidine and ganciclovir.
Article
Nutrition & Dietetics
Carlos Gonzalez-Quilen, Carme Grau-Bove, Rosa Jorba-Martin, Aleidis Caro-Tarrago, Montserrat Pinent, Anna Ardevol, Raul Beltran-Debon, Ximena Terra, M. Teresa Blay
Summary: The study assessed the protective effects of grape-seed proanthocyanidin extract (GSPE) on human colon dysfunction induced by DSS. Results demonstrated that preventive GSPE treatment dose-dependently reduced DSS-induced TEER loss, FD4 permeation, and TNF-alpha release in the proximal colon, while the distal colon showed no response to the treatment. Further studies are needed to explore the potential therapeutic application of GSPE in colonic dysfunction associated with metabolic disorders and inflammatory bowel disease.
EUROPEAN JOURNAL OF NUTRITION
(2021)
Article
Chemistry, Medicinal
Janneke Keemink, Erik Sjogren, Rene Holm, Christel A. S. Bergstrom
JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Article
Pharmacology & Pharmacy
Tycho Heimbach, Sandra Suarez-Sharp, Maziar Kakhi, Nico Holmstock, Andres Olivares-Morales, Xavier Pepin, Erik Sjogren, Eleftheria Tsakalozou, Paul Seo, Min Li, Xinyuan Zhang, Ho-Pi Lin, Timothy Montague, Amitava Mitra, Denise Morris, Nikunjkumar Patel, Filippos Kesisoglou
Article
Medicine, Research & Experimental
Johanna Eriksson, Helena Thorn, Erik Sjogren, Lisa Holmsten, Katarina Rubin, Hans Lennernas
MOLECULAR PHARMACEUTICS
(2019)
Article
Anesthesiology
Erik Sjogren, Lars Stahle, Hans Quiding, Bror Jonzon, Magnus M. Halldin, Anna K. Sundgren
EUROPEAN JOURNAL OF PAIN
(2019)
Article
Pharmacology & Pharmacy
D. Dahlgren, C. Roos, K. Peters, A. Lundqvist, C. Tannergren, E. Sjogren, M. Sjoblom, H. Lennernas
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2019)
Article
Pharmacology & Pharmacy
C. Roos, D. Dahlgren, E. Sjogren, M. Sjoblom, M. Hedeland, H. Lennernas
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2019)
Article
Pharmacology & Pharmacy
I. R. Dubbelboer, D. Dahlgren, E. Sjogren, H. Lennernas
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2019)
Article
Pharmacology & Pharmacy
Emelie Ahnfelt, Oliver Degerstedt, Elsa Lilienberg, Erik Sjogren, Per Hansson, Hans Lennernas
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2019)
Article
Pharmacology & Pharmacy
Johanna Eriksson, Erik Sjogren, Hans Lennernas, Helena Thorn
Article
Pharmacology & Pharmacy
B. Abrahamsson, M. McAllister, P. Augustijns, P. Zane, J. Butler, R. Holm, P. Langguth, A. Lindahl, A. Muellertz, X. Pepin, A. Rostami-Hodjegan, E. Sjogren, M. Berntsson, H. Lennernas
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2020)
Article
Chemistry, Medicinal
Xavier J. H. Pepin, Jennifer Dressman, Neil Parrott, Poonam Delvadia, Amitava Mitra, Xinyuan Zhang, Andrew Babiskin, Vidula Kolhatkar, Paul Seo, Lynne S. Taylor, Erik Sjogren, James M. Butler, Edmund Kostewicz, Christer Tannergren, Mirko Koziolek, Filippos Kesisoglou, Andre Dallmann, Yang Zhao, Sandra Suarez-Sharp
Summary: This paper summarizes the discussions on Day 1 of the workshop, which mainly focused on the best practices and challenges in measuring in vitro inputs needed for modeling, such as drug solubility, dissolution rate, potential precipitation, and drug permeability.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Correction
Pharmacology & Pharmacy
Johanna Eriksson, Erik Sjogren, Hans Lennernas, Helena Thorn
Article
Chemistry, Medicinal
Amitava Mitra, Sandra Suarez-Sharp, Xavier J. H. Pepin, Talia Flanagan, Yang Zhao, Evangelos Kotzagiorgis, Neil Parrott, Satish Sharan, Christophe Tistaert, Tycho Heimbach, Banu Zolnik, Erik Sjogren, Fang Wu, Om Anand, Shefali Kakar, Min Li, Shereeni Veerasingham, Shinichi Kijima, Gustavo Mendes Lima Santos, Baoming Ning, Kimberly Raines, Greg Rullo, Haritha Mandula, Poonam Delvadia, Jennifer Dressman, Paul A. Dickinson, Andrew Babiskin
Summary: This report summarizes the discussions on translational modeling strategies to support drug product development, manufacturing changes and controls on Day 3 of the workshop. The focus was on patient-centric product development and the role of PBBM in establishing drug product specifications and its practical applications in drug product quality. Breakout session discussions covered topics related to terminology, regulatory harmonization, CRDPS approaches, and bridging between biorelevant and quality control dissolution methods.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Johanna Eriksson, Helena Thorn, Hans Lennernas, Erik Sjogren
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2020)
Review
Chemistry, Medicinal
David Dahlgren, Erik Sjogren, Hans Lennernas