Article
Engineering, Biomedical
Dounia Dems, Ronit Freeman, Kyle D. Riker, Thibaud Coradin, Samuel Stupp, Carole Aime
Summary: By combining peptide amphiphile nanofibers and silica nanoparticles, it is possible to design biomaterials with tunable bioactive epitopes for improved cell adhesion and spreading on hydrogels. The synergy between different integrin-binding sequences on nanoparticle surfaces can enhance cell adhesion in composite materials with soft and hard nanostructures.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Medicinal
Chaitanya Kumar Thota, Dorian J. Mikolajczak, Christian Roth, Beate Koksch
Summary: The study presents a new peptidic scaffold, FF03, for the multivalent presentation of antimicrobial peptides (AMPs) to obtain nonhemolytic and noncytotoxic antimicrobial systems with improved efficacy compared to unconjugated AMP analogues.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Anh N. Tran-Ly, Kevin J. De France, Patrick Rupper, Francis W. M. R. Schwarze, Carolina Reyes, Gustav Nystrom, Gilberto Siqueira, Javier Ribera
Summary: The article presents a new method using fungal melanin to develop melanized-cationic cellulose nano-fiber foams that can successfully remove pollutants in water systems and be recycled multiple times, highlighting the opportunity to combine sustainable polymers like cellulose with unique properties of fungal melanin for water purification.
Article
Engineering, Biomedical
Fangfang Chen, Yuhan Huang, Zhengyu Huang, Tingting Jiang, Zailin Yang, Jie Zeng, Aishun Jin, Hua Zuo, Cheng Zhi Huang, Chengde Mao
Summary: Short peptides are poor immunogens, but they can be enhanced by arranging immunogens in multivalency. In this study, a molecular vaccine design using DNA duplex was developed to induce robust immune responses. The DNA-peptide epitope platform represents a facile and generalizable strategy to enhance immune response.
ACTA BIOMATERIALIA
(2023)
Article
Polymer Science
Peng He, Minghao Yang, Yu Lei, Lei Guo, Yan Wang, Gang Wei
Summary: Designing functional membranes through the collaboration of multi-dimensional nanomaterials is proposed in this study. A facile and green synthetic strategy is presented, involving graphene oxide (GO), peptides, and silver nanoparticles (AgNPs), for the synthesis of functional hybrid membranes with favorable antibacterial effects. The hybrid membranes are characterized and analyzed, and their excellent antibacterial performances are demonstrated through experiments.
Article
Chemistry, Multidisciplinary
Jian Bin Zhen, Jiajia Yi, Huan Huan Ding, Ke-Wu Yang
Summary: This study reports a positively charged nanocomposite PNs-Cur with broad-spectrum and efficient antibacterial activities against drug-resistant bacteria. It can disrupt the membrane potential of bacteria, change their membrane structure, and release curcumin to exert bactericidal effects, ultimately leading to irreversible bacterial apoptosis. PNs-Cur provides a novel strategy to develop efficient antibacterial materials.
Article
Biochemistry & Molecular Biology
Hanna Zhukouskaya, Pablo M. Blanco, Zulfiya Cernochova, Lucie Ctverackova, Roman Stano, Ewa Pavlova, Miroslav Vetrik, Peter Cernoch, Marcela Filipova, Miroslav Slouf, Miroslav Stepanek, Martin Hruby, Peter Kosovan, Jiri Panek
Summary: The researchers developed acid-functionalized glycogen conjugates as carriers for efficient encapsulation and inhibition of honeybee venom. Sufficiently functionalized conjugates electrostatically attract venom, resulting in its efficient encapsulation in a broad pH range. These functionalized glycogens are promising carriers for cationic molecular cargos or antidotes against animal venoms, as they possess biodegradability and biocompatibility.
Article
Chemistry, Multidisciplinary
Woo-Jin Jeong, Jiyoon Bu, Roya Jafari, Pavel Rehak, Luke J. Kubiatowicz, Adam J. Drelich, Randall H. Owen, Ashita Nair, Piper A. Rawding, Michael J. Poellmann, Caroline M. Hopkins, Petr Kral, Seungpyo Hong
Summary: A set of engineering strategies were developed to systematically enhance multivalent binding of peptides. The use of PAMAM dendrimers increased local peptide densities on a substrate, resulting in hierarchically multivalent architectures displaying various configurations.
Article
Chemistry, Multidisciplinary
Yue Wu, Ho-Fai Chau, Yik-Hoi Yeung, Waygen Thor, Hei-Yui Kai, Wai-Lun Chan, Ka-Leung Wong
Summary: In this study, a novel synthetic method for the efficient preparation of prefunctionalized core porphyrins and the controllable construction of various valent porphyrin-peptide conjugates was reported. Compared to traditional methods, this approach is faster, greener, and requires only one final-stage purification.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Engineering, Biomedical
Ziv Azoulay, Polina Aibinder, Ayala Gancz, Jacob Moran-Gilad, Shiri Navon-Venezia, Hanna Rapaport
Summary: FKF, a tripeptide with potential antimicrobial activity, forms a pure peptide hydrogel phase made of natural amino acids. The hydrogel exhibits bactericidal activity against E. coli and P. aeruginosa, and reduces P. aeruginosa contamination in a rat skin lesion model.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Hawk Leong Yuen, Szn Yi Chan, Yi En Ding, Suxing Lim, Gim Cheong Tan, Chiew Ling Kho
Summary: This study aimed to develop a potent antibacterial peptide (ABP) with minimal toxicity through phage display selection and computer-assisted modification. A peptide called PAM-5 with enhanced antibacterial features was obtained by modifying the peptide selected for its high affinity to Pseudomonas aeruginosa cell surface using an online peptide database. PAM-5 showed activity against various Gram-negative and selected Gram-positive bacteria, and was stable in human plasma without toxic effects on human cells and erythrocytes.
Article
Chemistry, Multidisciplinary
Jingjing Lei, Fei Yu, Haijiao Xie, Jie Ma
Summary: Capacitive deionization (CDI) technology is expected to address scaling, corrosion, and biofouling simultaneously in circulating cooling water systems. In this study, a flexible self-supporting Ti3C2Tx MXene/carbon nanofiber film with high-performance antifouling and antibacterial activity was fabricated. The film had a 3D interconnected conductive network, which expedited electron and ion transport and diffusion. The Ti3C2Tx/CNF-14 film exhibited high desalination capacity, rapid desalination rate, and impressive inactivation efficiency against Escherichia coli. These findings highlight the potential use of high-performance multifunctional CDI electrode materials for treating circulating cooling water.
Article
Chemistry, Multidisciplinary
Somashree Bose, Yogita Dahat, Deepak Kumar, Saikat Haldar, Sujoy K. Das
Summary: In this study, a cationic metal nanoparticle-conjugated fusogenic nanoemulsion (CFusoN) was designed for the treatment of Staphylococcus aureus infection. The nanoemulsion showed a killing efficiency of 99.999% against the bacteria, without exhibiting toxicity to red blood cells and fibroblast cells. The CFusoN altered the bacterial surface charge, leading to bacterial death, and solubilized the bacterial membrane lipid bilayer. In an animal model of skin wound infection, CFusoN demonstrated superior wound cleansing efficacy compared to commercial wound cleansers.
MATERIALS HORIZONS
(2023)
Article
Polymer Science
Ao Chen, Elliot Chen, Edmund F. Palermo
Summary: The study focuses on the synthesis of cationic dendrons with pendant alkyl chains of varying lengths, which exhibit potent membrane-disrupting antibacterial activities. However, compared to previously studied ammonium-functionalized analogues, guanidinium-functionalized dendrons do not show significant benefits, with the highest selectivity index found in the ammonium series.
Article
Polymer Science
Yunys Perez-Betancourt, Bianca de Carvalho Lins Fernandes Tavora, Eliana L. Faquim-Mauro, Ana Maria Carmona-Ribeiro
Summary: In this study, biocompatible lipid polymer nanoparticles were investigated as immuno-adjuvants and found to induce both humoral and cellular antigen-specific immune responses, demonstrating high biocompatibility and antigen adsorption capability.
Article
Materials Science, Multidisciplinary
Fuat Topuz, Ashif Y. Shaikh, Mustafa O. Guler, Tamer Uyar
JOURNAL OF MATERIALS SCIENCE
(2020)
Article
Biochemical Research Methods
Muhammed Burak Demircan, Sehmus Tohumeken, Nuray Gunduz, Mohammad Aref Khalily, Turgay Tekinay, Mustafa O. Guler, Ayse B. Tekinay
BIOTECHNOLOGY JOURNAL
(2020)
Article
Peripheral Vascular Disease
H. H. Pulkkinen, M. Kiema, J. P. Lappalainen, A. Toropainen, M. Beter, A. Tirronen, L. Holappa, H. Niskanen, M. U. Kaikkonen, S. Yla-Herttuala, Johanna P. Laakkonen
Summary: BMP family members regulate VEGFR2 and Notch signaling via TAZ-Hippo pathway in endothelial tip and stalk cell formation. Among them, BMP6 is the first member found to directly regulate both Hippo signaling and neovessel formation, potentially serving as a target in pro/anti-angiogenic therapies.
Article
Biochemistry & Molecular Biology
Melike Sever-Bahcekapili, Canelif Yilmaz, Altan Demirel, Mustafa Cemil Kilinc, Ihsan Dogan, Yusuf Sukru Caglar, Mustafa O. Guler, Ayse B. Tekinay
Summary: The study demonstrates the use of peptide nanofibers as a promising approach for stimulating cellular regeneration in spinal cord injuries. These neuroactive nanofibers provide bioactive cues to enable neural regeneration and enhance tissue integrity in both in vitro and in vivo models. Treatment with these peptide nanofiber scaffolds also leads to significant behavioral improvement, suggesting their potential for facilitating regeneration in the white matter of the spinal cord.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Isidore Mushimiyimana, Henri Niskanen, Mustafa Beter, Johanna P. Laakkonen, Minna U. Kaikkonen, Seppo Yla-Herttuala, Nihay Laham-Karam
Summary: This study identified an endothelial super-enhancer that can significantly increase transgene expression and affect cell division-related pathways by regulating the ADAMTS18 gene, leading to substantial downstream effects on cell proliferation.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
M. Beter, A. Abdollahzadeh, H. H. Pulkkinen, H. Huang, F. Orsenigo, P. U. Magnusson, S. Yla-Herttuala, J. Tohka, J. P. Laakkonen
Summary: SproutAngio, a Python-based open-source tool, was developed for the automated segmentation and analysis of endothelial lumen space and sprout morphology. It outperforms the widely used ImageJ plugin and provides new methods for endothelial lumen analysis. SproutAngio can contribute to the understanding of blood vessel formation and heterogeneity.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Kenana Dayob, Aygul Zengin, Ruslan Garifullin, Mustafa O. Guler, Timur I. Abdullin, Abdulla Yergeshov, Diana V. Salakhieva, Hong Hanh Cong, Mohamed Zoughaib
Summary: This study demonstrates the design of self-assembling nanofiber scaffolds based on peptide amphiphile molecules containing multi-functional histidine residues. The study explores the self-assembly of peptide amphiphiles and the characteristics of nanofiber scaffolds, as well as their interaction with essential microelements such as zinc, copper, and manganese. The effects of metal-activated peptide amphiphile scaffolds on mammalian cell behavior and reactive oxygen species levels were investigated. The results reveal the ability of these scaffolds to modulate cell adhesion, proliferation, and morphological differentiation, particularly through the role of manganese in cell-matrix interaction and neuritogenesis. This study provides proof-of-concept for the development of histidine-functionalized peptide nanofiber scaffolds activated with reactive oxygen species-modulating metals to induce regenerative responses.
Article
Pharmacology & Pharmacy
Seher Yaylaci, Erdem Dinc, Bahri Aydin, Ayse B. Tekinay, Mustafa O. Guler
Summary: In this study, self-assembled hydrogels composed of peptide amphiphile molecules were developed for sustained release of ranibizumab in the treatment of wet age-related macular degeneration. The hydrogel system showed extended release patterns of ranibizumab without dose dumping, and the released drug was biologically functional and effective in blocking angiogenesis. In vivo experiments demonstrated that the drug released from the hydrogel nanofiber system had longer retention time in the posterior chamber of the rabbit eye compared to a control group. The peptide-based hydrogel nanofiber delivery system holds promise for intravitreal anti-VEGF drug delivery in clinics.
Article
Chemistry, Multidisciplinary
Jovana Mitrovic, Gabriella Richey, Sarah Kim, Mustafa O. O. Guler
Summary: Peptides serve as versatile building blocks for the fabrication of various nanostructures, such as hydrogels and nanoparticles. Precise chemical functionalization allows for controlled bioactivity and physical properties, enabling the development of functional materials. The intermolecular interactions and intrinsic peptide characteristics are determined by the conjugation of small molecules on amino acid side chains. This Perspective discusses peptide building blocks, nanostructure formation mechanisms, and the properties of these peptide materials, providing insights from recent publications. Bioinstructive and stimuli-responsive peptide materials have significant impacts in the field of nanomedicine, including drug delivery, cellular engineering, regenerative medicine, and biomedicine.
Article
Materials Science, Biomaterials
Maria Lopes, Marilia Torrado, Daryl Barth, Sofia D. Santos, Melike Sever-Bahcekapili, Ayse B. Tekinay, Mustafa O. Guler, Franck Cleymand, Ana P. Pego, Joao Borges, Joao F. Mano
Summary: Peptide amphiphiles (PAs) are effective molecular building blocks for self-assembling nanobiomaterials. This study developed a simple approach to recreate the native neural extracellular matrix (ECM) using electrostatic-driven supramolecular presentation of a laminin-derived IKVAV-containing self-assembling PA (IKVAV-PA). The bioactive ECM-mimetic supramolecular nanofilms promote primary neuronal cells' adhesion, viability, morphology, and neurite outgrowth, making them promising for neural tissue regeneration.
BIOMATERIALS SCIENCE
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
A. Ravindran, L. Holappa, H. Niskanen, M. Beter, M. Kiema, I. Skovorodkin, S. Vainio, S. Yla-Herttuala, J. P. Laakkonen, T. Ord, M. U. Kaikkonen
Article
Hematology
Oscar Hsin-Fu Liu, Miika Kiema, Mustafa Beter, Seppo Yla-Herttuala, Johanna P. Laakkonen, Minna U. Kaikkonen
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2020)
Article
Nanoscience & Nanotechnology
Thai Duong Luong, Mohamed Zoughaib, Ruslan Garifullin, Svetlana Kuznetsova, Mustafa O. Guler, Timur Abdullin
ACS APPLIED BIO MATERIALS
(2020)
Article
Nanoscience & Nanotechnology
Ozge Uysal, Elif Arslan, Gulcihan Gulseren, Mustafa Cemil Kilinc, Ihsan Dogan, Hakan Ozalp, Yusuf Sukru Caglar, Mustafa O. Guler, Ayse B. Tekinay
ACS APPLIED BIO MATERIALS
(2019)
Review
Pharmacology & Pharmacy
Gozde Uzunalli, Mustafa O. Guler
THERAPEUTIC DELIVERY
(2020)