Article
Biochemistry & Molecular Biology
Md. Abdullah-Al-Kamran Khan, Abul Bashar Mir Md. Khademul Islam
Summary: The virus can enhance its survival by manipulating key signaling pathways in host cells, leading to more complex host responses to the virus, which may result in pathological abnormalities in the lungs and other organs, as well as an increased risk of infection for patients with comorbidities.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Alexandr D. Efimov, Alla K. Golomidova, Eugene E. Kulikov, Ilya S. Belalov, Pavel A. Ivanov, Andrey Letarov
Summary: Most enterobacterial O antigen types provide strong protection against direct recognition by bacteriophage receptor-recognition proteins. Certain bacteriophages use specific O antigens as their primary receptors and can also bind to additional receptors to infect different strains of E. coli. The mechanical force of the deployment of short tail fibers allows these phages to penetrate the O polysaccharide layer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Eugene E. Kulikov, Alla K. Golomidova, Alexandr D. Efimov, Ilya S. Belalov, Maria A. Letarova, Evelina L. Zdorovenko, Yuriy A. Knirel, Andrei S. Dmitrenok, Andrey Letarov
Summary: Tailed bacteriophages are predominant in the intestinal viromes of vertebrate animals, but the distinction between lytic and lysogenic lifestyles can vary between species or individuals. The human viromes are mostly dominated by temperate phages, while in horse feces, virulent phages are more common. The Hf4s temperate coliphage isolated from horse feces has unique characteristics, such as recognizing the host O antigen as its primary receptor and possessing a seroconversion cluster that protects lysogens from superinfection by some virulent coliphages.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Shweta Singh, Jonathan J. Wilksch, Rhys A. Dunstan, Anna Mularski, Nancy Wang, Dianna Hocking, Leila Jebeli, Hanwei Cao, Abigail Clements, Adam W. J. Jenney, Trevor Lithgow, Richard A. Strugnell
Summary: This study explores the mechanisms and forces that enable encapsulated bacteria to retain their capsules, which are crucial for their virulence. The researchers discovered that interactions between surface carbohydrate polymers, especially the O antigen of the lipopolysaccharide molecule, enhance encapsulation in Klebsiella pneumoniae. The loss of the enzyme WaaL, responsible for ligation, resulted in reduced capsule retention and decreased virulence. This finding suggests that targeting WaaL could be a potential strategy for developing antimicrobials against drug-resistant pathogens like K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yahong Tan, Wenxia Song, Lijuan Gao, Weican Zhang, Xuemei Lu
Summary: This study revealed that chu_2177 is crucial for cellulose utilization in C. hutchinsonii. Its deletion resulted in O-antigen deficiency and affected cellulose degradation, cell motility, biofilm formation, and stress resistance. Furthermore, the deletion of chu_2177 affected the localization of endoglucanase and T9SS cargo proteins on the outer membrane.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ayumi Kato, Mana Ito, Takao Sanaki, Tomohiko Okuda, Noriko Tsuchiya, Ryo Yoshimoto, Hideo Yukioka
Summary: The study revealed that long-chain acyl-CoA synthetase 1 (Acsl1) plays a crucial role in the formation of skin barrier, and its deficiency can lead to embryonic lethality. In Acsl1-deficient mice, the essential lipid Cer[EOS] was reduced, while its precursor Cer[OS] was increased.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Biochemistry & Molecular Biology
Lukas Opalka, Jason M. Meyer, Veronika Ondrejcekova, Linda Svatosova, Franz P. W. Radner, Katerina Vavrova
Summary: This study explored the importance of the linoleate moiety in epidermal omega-O-acylceramides (omega-O-acylCers) in the assembly of the skin lipid barrier. The results showed that the linoleate moiety is essential for lamellar pairing in skin lipid structures, and omega-hydroxylated Cers (omega-OHCers) cannot substitute for omega-O-acylCers. These findings suggest that omega-O-acylCer supplementation may be a potential therapeutic option for patients with PNPLA1 deficiency.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Nutrition & Dietetics
Geok Lin Khor, Siew Siew Lee
Summary: The study found that children mainly consumed cereals, with their energy and protein intake exceeding the Recommended Nutrient Intakes for Malaysia, but their intake of various vitamins and minerals was insufficient. This imbalanced dietary intake could lead to health issues, highlighting the need for interventions to improve child feeding knowledge and practices.
Article
Biochemistry & Molecular Biology
Laura Maggi, Izabella Chrystina Rocha, Genil Mororo Araujo Camelo, Vanessa Rodrigues Fernandes, Deborah Negrao-Correa
Summary: This study compared the immune response and granuloma formation in wild-type BALB/c mice and BALB/c mice genetically deficient in the IL-33 receptor when experimentally infected with Schistosoma mansoni. The results showed that the absence of the IL-33/ST2 pathway did not affect the Schistosoma-induced Th2 response, but was crucial in modulating granuloma-mediated pathology.
Article
Microbiology
Andreas Schmidt, Wolfgang Rabsch, Nina K. Broeker, Stefanie Barbirz
Article
Virology
Nina K. Broeker, Franziska Kiele, Sherwood R. Casjens, Eddie B. Gilcrease, Anja Thalhammer, Joachim Koetz, Stefanie Barbirz
Article
Chemistry, Multidisciplinary
Vasil N. Georgiev, Andrea Grafmueller, David Bleger, Stefan Hecht, Sonja Kunstmann, Stefanie Barbirz, Reinhard Lipowsky, Rumiana Dimova
Article
Chemistry, Multidisciplinary
Sonja Kunstmann, Ulrich Gohlke, Nina K. Broeker, Yvette Roske, Udo Heinemann, Mark Santer, Stefanie Barbirz
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Virology
Sonja Kunstmann, Tom Scheidt, Saskia Buchwald, Alexandra Helm, Laurence A. Mulard, Angelika Fruth, Stefanie Barbirz
Article
Biochemistry & Molecular Biology
Nina K. Broeker, Yvette Roske, Angelo Valleriani, Mareike S. Stephan, Dorothee Andres, Joachim Koetz, Udo Heinemann, Stefanie Barbirz
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Valentin Dunsing, Tobias Irmscher, Stefanie Barbirz, Salvatore Chiantia
Article
Chemistry, Multidisciplinary
Sonja Kunstmann, Olof Engstrom, Marko Wehle, Goran Widmalm, Mark Santer, Stefanie Barbirz
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Microbiology
Mareike S. Stephan, Nina K. Broeker, Athanasios Saragliadis, Norbert Roos, Dirk Linke, Stefanie Barbirz
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Tobias Irmscher, Yvette Roske, Igor Gayk, Valentin Dunsing, Salvatore Chiantia, Udo Heinemann, Stefanie Barbirz
Summary: Pathogenic microorganisms often reside in glycan-based biofilms, which are regulated by bacterial communities through small-molecule signaling. Extracellular glycosidases play a crucial role in the reorganization or degradation of the glycan matrix.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Medicine, Research & Experimental
Carola Venturini, Aleksandra Petrovic Fabijan, Alicia Fajardo Lubian, Stefanie Barbirz, Jonathan Iredell
Summary: Bacteriophages are selective viral predators of bacteria and can be used to treat bacterial infections. However, there are challenges to implementing phage therapy into medical practice, including a lack of clinical trial data and a need for a better understanding of bacterium-phage interactions.
EMBO MOLECULAR MEDICINE
(2022)
Article
Biophysics
Mareike S. Stephan, Valentin Dunsing, Shreya Pramanik, Salvatore Chiantia, Stefanie Barbirz, Tom Robinson, Rumiana Dimova
Summary: Gram-negative bacteria have a cell wall that includes lipids, proteins, and glycans, forming a protective layer. The outer membrane contains lipopolysaccharides (LPSs), which play a significant role. Incorporating LPS into artificial membranes is challenging due to their water solubility. This study introduces a new emulsification step to incorporate LPS into specific leaflets of giant unilamellar vesicles (GUVs) and quantifies the LPS content and membrane asymmetry. Diffusion coefficient measurements show that increased LPS concentrations in the membranes lead to decreased diffusivity.
BIOPHYSICAL JOURNAL
(2023)
Meeting Abstract
Biophysics
Mareike Stephan, Stefanie Barbirz, Tom Robinson, Naresh Yandrapalli, Rumiana Dimova
BIOPHYSICAL JOURNAL
(2021)