期刊
MOLECULAR ECOLOGY RESOURCES
卷 18, 期 2, 页码 306-318出版社
WILEY
DOI: 10.1111/1755-0998.12725
关键词
18S SSU rRNA gene; barcoding gap; dark-spored myxomycetes; DNA barcoding; hidden diversity; Myxogastria; OTU threshold; species concept; species diversity
资金
- German Science Foundation, DFG [GRK 2010, UN262/9-1]
- Russian Founds for Basic Research [RFBR 15-29-02622]
Unicellular, eukaryotic organisms (protists) play a key role in soil food webs as major predators of microorganisms. However, due to the polyphyletic nature of protists, no single universal barcode can be established for this group, and the structure of many protistean communities remains unresolved. Plasmodial slime moulds (Myxogastria or Myxomycetes) stand out among protists by their formation of fruit bodies, which allow for a morphological species concept. By Sanger sequencing of a large collection of morphospecies, this study presents the largest database to date of dark-spored myxomycetes and evaluate a partial 18S SSU gene marker for species annotation. We identify and discuss the use of an intraspecific sequence similarity threshold of 99.1% for species differentiation (OTU picking) in environmental PCR studies (ePCR) and estimate a hidden diversity of putative species, exceeding those of described morphospecies by 99%. When applying the identified threshold to an ePCR data set (including sequences from both NGS and cloning), we find 64 OTUs of which 21.9% had a direct match (>99.1% similarity) to the database and the remaining had on average 90.2 +/- 0.8% similarity to their best match, thus thought to represent undiscovered diversity of dark-spored myxomycetes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据