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Cell Biology
Qiuhong Shen, Yin Shi, Jiaqi Liu, Hua Su, Jingtao Huang, Yi Zhang, Chao Peng, Tianhua Zhou, Qiming Sun, Wei Wan, Wei Liu
Summary: In this study, it was found that the acetylation of STX17 regulates its SNARE activity and autophagic degradation, affecting the fusion of autophagosomes with lysosomes and promoting the degradation of autophagic cargoes. These findings suggest a mechanism by which acetylation regulates late-stage autophagy and other STX17-related intracellular membrane fusion events.
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Cell Biology
Guangyan Miao, Hongyu Zhao, Yan Li, Mingming Ji, Yong Chen, Yi Shi, Yuhai Bi, Peihui Wang, Hong Zhang
Summary: ORF3a of the COVID-19 virus SARS-CoV-2 inhibits autophagy activity by blocking fusion of autophagosomes/amphisomes with lysosomes. This leads to accumulation of autophagosomes/amphisomes, impaired lysosome function, and ultimately blocks autophagy process.
DEVELOPMENTAL CELL
(2021)
Article
Cell Biology
Sara Monaci, Federica Coppola, Daniela Rossi, Gaia Giuntini, Irene Filippi, Giuseppe Marotta, Silvano Sozzani, Fabio Carraro, Antonella Naldini
Summary: Hypoxia-induced autophagy in dendritic cells is regulated by PI3Ks, especially Vps34. The study highlights the role of autophagy in maintaining DC homeostasis and inflammatory response levels.
Review
Cell Biology
Yanfei He, Huaqing Lu, Yuting Zhao
Summary: Autophagy activation plays a crucial role in many diseases, but the development of specific activators remains a challenge. Tat-BECN1 peptide is a candidate specific activator that initiates autophagy through specific molecular mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhongliang Hu, Yamei Luo, Yuting Liu, Yaru Luo, Liangce Wang, Shengsong Gou, Yuling Peng, Rui Wei, Da Jia, Yuan Wang, Shangbang Gao, Yan Zhang
Summary: The increase in global life expectancy is not accompanied by an increase in health span, requiring a better understanding of age-related decline in behavior. A genome-wide screening in Caenorhabditis elegans identified 34 genes as potential regulators of motor aging, with VPS-34 being crucial in aged but not young worms. This phosphatidylinositol 3-kinase primarily functions in aged motor neurons by regulating neurotransmission, and inhibition of VPS-34 improves motor aging in worms and mice.
Article
Biochemistry & Molecular Biology
Jing Hao, Haimei Liu, Jiawen Wang, Xiuzhen Wang, Chunxia Huang, Lijuan Liang, Jing Chen, Yi Wang, Yunjun Liu
Summary: Three new iridium (III) complexes were synthesized and showed excellent cytotoxicity against various cancer cells, especially human cervical carcinoma HeLa cells. The complexes effectively inhibited cell colony formation and deep penetration into HeLa 3D multicellular tumor spheroids, resulting in notable cytotoxic effects. The rapid uptake of the complexes and their localization mainly at the mitochondria were observed through confocal microscopy. Mechanistic studies revealed that the complexes promoted excessive generation of reactive oxygen species (ROS), disrupted the intracellular redox balance, induced oxidative stress, and led to caspase-dependent apoptosis. In addition, the complexes induced cell apoptosis by inactivating the PI3K/AKT/mTOR pathway, causing DNA damage, and suppressing cyclin D1/CDK4/6 activity to arrest the cell cycle in the G0/G1 phase. The in vivo assays on a xenografted cervical carcinoma mouse model confirmed the excellent biocompatibility and antineoplastic efficiency of one of the complexes (Ir3). Overall, this work provides a foundation for the development of iridium (III) complexes with potential clinical applications.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Yarong Liu, Aiwei Song, Hongfei Wu, Yin Sun, Min Dai
Summary: Paeonol induces autophagy in VSMCs by activating the III class PI3K/Beclin-1 signaling pathway, ultimately inhibiting VSMCs apoptosis.
Article
Biochemistry & Molecular Biology
Hao Liu, Shuangyu Dong, Ming Li, Fengwei Gu, Guili Yang, Tao Guo, Zhiqiang Chen, Jiafeng Wang
Summary: The study revealed the important role of OsPrx30 in rice immunity against Xanthomonas oryzae pv. oryzae, showing that overexpression of OsPrx30 increases susceptibility to the pathogen. Furthermore, transcription of OsPrx30 is regulated by the AT-hook transcription factor OsATH1, and depletion of OsATH1 results in increased susceptibility to the pathogen.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Runan Yan, Wei Gao, Wenjia Chen, Yue Liu, Li Shen, Yue Dai, Rui Xu, Qing Chang, Yu Fu, Yong Zhao
Summary: TFPI plays a protective role in cardiomyocyte ischemia reperfusion (I/R) injury. It reduces cardiomyocyte damage by inhibiting autophagy and alleviating oxidative stress.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ludovica Nucci, Caterina Costanzo, Marco Carfora, Fabrizia d'Apuzzo, Lorenzo Franchi, Letizia Perillo
Summary: The optimal timing of intervention for the treatment of Class III malocclusion with early Class III protocol did not show significant differences based on different cervical vertebral maturation stages. Post-treatment outcomes were favorable regardless of the stage of maturation from CS1 to CS4.
PROGRESS IN ORTHODONTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Emad Eddin Alzoubi, Simon Camilleri, Mohammed Al Muzian, Nikolai Attard
Summary: This was a randomized, controlled trial comparing the effectiveness of dental and skeletal anchorage in treating maxillary hypoplasia. The results showed that both tooth-borne and skeletally anchored FM/Alt-RAMEC had similar positive effects on skeletal and dental changes.
EUROPEAN JOURNAL OF ORTHODONTICS
(2023)
Editorial Material
Cell Biology
Xiawei Cheng, Qiming Sun
Article
Biochemistry & Molecular Biology
Xiawei Cheng, Xiuling Ma, Qi Zhu, Dandan Song, Xianming Ding, Lin Li, Xiao Jiang, Xinyi Wang, Rui Tian, Hua Su, Zhirong Shen, She Chen, Ting Liu, Weihua Gong, Wei Liu, Qiming Sun
Article
Cell Biology
Xianming Ding, Xiao Jiang, Rui Tian, Pengwei Zhao, Lin Li, Xinyi Wang, She Chen, Yushan Zhu, Mei Mei, Shilai Bao, Wei Liu, Zaiming Tang, Qiming Sun
Article
Biochemistry & Molecular Biology
Xiao Jiang, Xinyi Wang, Xianming Ding, Mengjie Du, Boran Li, Xialian Weng, Jingzi Zhang, Lin Li, Rui Tian, Qi Zhu, She Chen, Liang Wang, Wei Liu, Lei Fang, Dante Neculai, Qiming Sun
Article
Cell Biology
Qiuhong Shen, Yin Shi, Jiaqi Liu, Hua Su, Jingtao Huang, Yi Zhang, Chao Peng, Tianhua Zhou, Qiming Sun, Wei Wan, Wei Liu
Summary: In this study, it was found that the acetylation of STX17 regulates its SNARE activity and autophagic degradation, affecting the fusion of autophagosomes with lysosomes and promoting the degradation of autophagic cargoes. These findings suggest a mechanism by which acetylation regulates late-stage autophagy and other STX17-related intracellular membrane fusion events.
Article
Pharmacology & Pharmacy
Jianxiang Chen, Yiting Qiao, Guo Chen, Cunjie Chang, Heng Dong, Bo Tang, Xiawei Cheng, Xiufeng Liu, Zichun Hua
Summary: The study confirmed the active involvement of host immune response in Salmonella-mediated cancer therapy, highlighting the key role of flagella in activating the host immune response.
ACTA PHARMACEUTICA SINICA B
(2021)
Review
Cell Biology
Yi Zhang, Jiayu Gu, Qiming Sun
Summary: Cells regulate the relationship between stress granules and protein aggregates through the autophagy pathway to maintain cellular homeostasis. Defective aggrephagy is associated with various neurodegenerative diseases, including ALS.
Article
Cell Biology
Shulin Li, Rui Yan, Jialu Xu, Shiqun Zhao, Xinyu Ma, Qiming Sun, Min Zhang, Ying Li, Jun-Jie Gogo Liu, Liangyi Chen, Sai Li, Ke Xu, Liang Ge
Summary: Under stress, the endomembrane system reorganizes to support autophagosome biogenesis by forming a new type of membrane contact between the ER-Golgi intermediate compartment (ERGIC) and the ER-exit site (ERES). This contact is established by the interaction between TMED9 and SEC12, generating a short distance opposition between the two compartments, which promotes the formation of the autophagosome precursor.
Article
Cell Biology
Hong Huang, Qinqin Ouyang, Kunrong Mei, Ting Liu, Qiming Sun, Wei Liu, Rong Liu
Summary: This study reveals that both acetylation and phosphorylation modifications control the function of SCFD1 in autophagosome-lysosome fusion. KAT2B/PCAF catalyzes the acetylation of SCFD1, while SIRT4 deacetylates it. Additionally, AMPK-controlled phosphorylation disrupts the interaction between SCFD1 and KAT2B and inhibits SCFD1 acetylation. Furthermore, SCFD1 acetylation inhibits autophagic flux by blocking SNARE complex formation.
Article
Cell Biology
Xinyi Wang, Xiao Jiang, Boran Li, Jiahua Zheng, Jiansheng Guo, Lei Gao, Mengjie Du, Xialian Weng, Lin Li, She Chen, Jingzi Zhang, Lei Fang, Ting Liu, Liang Wang, Wei Liu, Dante Neculai, Qiming Sun
Summary: The study reveals that the ER-phagy receptor FAM134B is acetylated by CBP acetyltransferase and phosphorylated by CAMKII, leading to the degradation of the endoplasmic reticulum (ER). On the other hand, SIRT7 deacetylates FAM134B to reduce its activity in ER degradation. This work provides mechanistic insights into how ER-phagy is regulated and controlled.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Mengshu Jia, Xiao Yue, Weixia Sun, Qianjun Zhou, Cheng Chang, Weihua Gong, Jian Feng, Xie Li, Ruonan Zhan, Kemin Mo, Lijuan Zhang, Yajie Qian, Yuying Sun, Aoxue Wang, Yejun Zou, Weicai Chen, Yan Li, Li Huang, Yi Yang, Yuzheng Zhao, Xiawei Cheng
Summary: In this study, it was found that lactate dehydrogenase A (LDHA) is activated upon UNC-51-like kinase 1 (ULK1) activation under nutrient deprivation, and promotes lactate production through Vps34 lactylation. These findings describe the interplay between autophagy and glycolysis.
Editorial Material
Cell Biology
Weixia Sun, Mengshu Jia, Yingyan Feng, Xiawei Cheng
Summary: Lactate, produced from pyruvate by LDH, plays a significant role in physiological and pathological processes. Recent research has shown that lactate regulates autophagy by phosphorylating LDHA and lactylating PIK3C3/VPS34, promoting autophagy and affecting skeletal muscle homeostasis and cancer progression.
Article
Multidisciplinary Sciences
Xufeng Cen, Yanying Chen, Xiaoyan Xu, Ronghai Wu, Fusheng He, Qingwei Zhao, Qiming Sun, Cong Yi, Jie Wu, Ayaz Najafov, Hongguang Xia
NATURE COMMUNICATIONS
(2020)
Article
Medicine, Research & Experimental
Chen Liu, Xiawei Cheng, Juntao Chen, Yao Wang, Xiaoying Wu, Rui Tian, Baoqing Liu, Xianfeng Ding, Qiming Sun, Weihua Gong