期刊
MOLECULAR CELL
卷 65, 期 6, 页码 1109-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2017.02.007
关键词
-
资金
- U.S. Department of Energy Computational Science Graduate Fellowship [DE-FG02-97ER25308]
- NIH through the National Institute of Mental Health (NIMH) [5DP1-MH100706, 1R01-MH110049]
- NSF
- New York Stem Cell
- Simons Foundation
- Paul G. Allen Family Foundation
- Vallee Foundation
- JST, PRESTO
- JSPS KAKENHI [26291010, 15H01463]
- Basic Science and Platform Technology Program for Innovative Biological Medicine from the Japan Agency for Medical Research and Development, AMED
- Council for Science, Technology and Innovation (CSTI)
- Cross-Ministerial Strategic Innovation Promotion Program (SIP)
- Technologies for Creating Next-Generation Agriculture, Forestry and Fisheries (Bio-Oriented Technology Research Advancement Institution, NARO)
- Platform for Drug Discovery, Informatics, and Structural Life Science from Ministry of Education, Culture, Sports, Science and Technology
- Grants-in-Aid for Scientific Research [26291010, 15H01463, 17H03640] Funding Source: KAKEN
The RNA-guided endonuclease Cas9 generates a double-strand break at DNA target sites complementary to the guide RNA and has been harnessed for the development of a variety of new technologies, such as genome editing. Here, we report the crystal structures of Campylobacter jejuni Cas9 (CjCas9), one of the smallest Cas9 orthologs, in complex with an sgRNA and its target DNA. The structures provided insights into a minimal Cas9 scaffold and revealed the remarkable mechanistic diversity of the CRISPR-Cas9 systems. The CjCas9 guide RNA contains a triple-helix structure, which is distinct from known RNA triple helices, thereby expanding the natural repertoire of RNA triple helices. Furthermore, unlike the other Cas9 orthologs, CjCas9 contacts the nucleotide sequences in both the target and non-target DNA strands and recognizes the 5'-NNNVRYM-3' as the protospacer-adjacent motif. Collectively, these findings improve our mechanistic understanding of the CRISPR-Cas9 systems and may facilitate Cas9 engineering.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据