Article
Biochemistry & Molecular Biology
Bruna Ribeiro Andrade Ramos, Julia Abbade Tronco, Marcio Carvalho, Tainara Francini Felix, Patricia Pintor Reis, Juliano Coelho Silveira, Marcia Guimaraes Silva
Summary: This study compared the expression of miRNAs in sEV from peripheral blood between term and preterm pregnancies. It found that miR-612 expression was increased in the preterm group, while miR-1253, miR-1283, miR378e, and miR-579-3p were downregulated in the preterm premature rupture of membranes (PPROM) group. The study suggests that miRNAs in circulating sEV play a regulatory role in the pathogenesis of preterm labor (PTL) and PPROM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Snehaa V. Seal, Mathilde Henry, Clementine Pajot, Cyrielle Holuka, Danielle Bailbe, Jamileh Movassat, Muriel Darnaudery, Jonathan D. Turner
Summary: Type-2 diabetes has an immune component, and the immune status outside the pancreas remains unclear. Goto-Kakizaki rats, a non-obese Type-2 diabetes model, have a limited pro-inflammatory baseline immune profile and marked immune dysfunction.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Paula Morales-Sanchez, Carmen Lambert, Jessica Ares-Blanco, Lorena Suarez-Gutierrez, Elsa Villa-Fernandez, Ana Victoria Garcia, Miguel Garcia-Villarino, Juan Ramon Tejedor, Mario F. Fraga, Edelmiro Menendez Torre, Pedro Pujante, Elias Delgado
Summary: This study aimed to analyze the circulating miRNA expression profile of patients with type 1 diabetes and no other associated pathology. The expression of hsa-miR-1-3p was found to be significantly increased in patients with type 1 diabetes and positively correlated with glycated haemoglobin levels. Changes in hsa-miR-1-3p were also found to directly affect genes involved in vascular development and cardiovascular pathologies. Circulating hsa-miR-1-3p, along with glycemic control, could be used as prognostic biomarkers in type 1 diabetes to prevent the development of vascular complications in these patients.
SCIENTIFIC REPORTS
(2023)
Article
Cardiac & Cardiovascular Systems
Vibhu Parcha, Nirav Patel, Orlando M. Gutierrez, Peng Li, Karen L. Gamble, Kiran Musunuru, Kenneth B. Margulies, Thomas P. Cappola, Thomas J. Wang, Garima Arora, Pankaj Arora
Summary: This clinical trial investigated the diurnal rhythmicity of natriuretic peptides in healthy lean and obese individuals, revealing a misalignment of the NP-BP diurnal rhythm in the obese population.
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Iveta Nedeva, Antoaneta Gateva, Yavor Assyov, Vera Karamfilova, Tsvetelina Velikova, Zdravko Kamenov
Summary: This study found that netrin-1 is associated with an increased risk for glycaemic disorders in patients with obesity. Circulating serum netrin-1 levels were significantly lower in patients with obesity, as well as in those with prediabetes and diabetes.
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yerim Heo, Hyunjung Kim, Jiwon Lim, Sun Shim Choi
Summary: The study investigates the differential expression of miRNAs in lean and obese conditions and identifies the types and degree of regulation of adipogenesis-related miRNAs. DEMs related to adipogenesis are more strongly downregulated in obese conditions compared to lean conditions, and there are more obese-specific downregulated DEMs.
SCIENTIFIC REPORTS
(2022)
Article
Physiology
Ceren Eyileten, Zofia Wicik, Alex Fitas, Mikolaj Marszalek, Jenny E. Simon, Salvatore De Rosa, Szczepan Wiecha, Jeffrey Palatini, Marek Postula, Lukasz A. Malek
Summary: The study evaluated the expression of miRNAs in ultramarathon runners before and after the race, showing significant increases in miR-125a-5p, miR-126, and miR-223 post-race. MiR-1-3p showed a negative correlation with hs-CRP levels post-race and was associated with faster race completion times.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Melissa Tomasi, Alessandro Cherubini, Serena Pelusi, Sara Margarita, Cristiana Bianco, Francesco Malvestiti, Lorenzo Miano, Stefano Romeo, Daniele Prati, Luca Valenti
Summary: This study investigates the relationship between circulating IL32 concentration and blood pressure control in individuals at high risk of metabolic dysfunction associated fatty liver disease (MAFLD). The results show that higher IL32 levels are independently associated with impaired blood pressure control, indicating a close association between IL32 concentration and cardiovascular disease risk.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Cincione Raffaele Ivan, Antonietta Messina, Giuseppe Cibelli, Giovanni Messina, Rita Polito, Francesca Losavio, Ester La Torre, Vincenzo Monda, Marcellino Monda, Stefano Quiete, Elias Casula, Nicola Napoli, Giuseppe Defeudis
Summary: Our study suggests that a very low-calorie ketogenic Mediterranean diet can rapidly improve metabolic, anthropometric, and body composition parameters in patients with prediabetes or diabetes and overweight/obesity.
Article
Cardiac & Cardiovascular Systems
Carl Johan Ostgren, Julia Otten, Karin Festin, Oskar Angeras, Goran Bergstrom, Kerstin Cederlund, Gunnar Engstrom, Maria J. Eriksson, Mats Eriksson, Tove Fall, Anders Gummesson, Emil Hagstrom, Urban Hellman, Stefan K. James, Tomas Jernberg, Johan Kihlberg, David Kylhammar, Hanna Markstad, Peter Nilsson, Anders Persson, Margaretha Persson, Carlo Pirazzi, Rebecka Renklint, Annika Rosengren, Stefan Soderberg, Johan Sundstrom
Summary: The study found that patients with prediabetes and diabetes have a greater burden of atherosclerosis in the coronary and carotid arteries compared to individuals with normal blood sugar levels.
CARDIOVASCULAR DIABETOLOGY
(2023)
Article
Immunology
Jie Song, Aole Li, Yu Qian, Bin Liu, Linshuoshuo Lv, Ding Ye, Xiaohui Sun, Yingying Mao
Summary: This study systematically evaluated the role of circulating cytokines on the risk of cancer using a genetic epidemiological approach. The results showed that genetically predicted high levels of interleukin-18 were associated with a decreased risk of acute myeloid leukemia, and circulating levels of IL-17 were associated with altered stomach cancer risk. Additionally, suggestive evidence for other cytokine-cancer associations was found.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Mohammad Shafi Kuchay, Jose Ignacio Martinez-Montoro, Narendra Singh Choudhary, Jose Carlos Fernandez-Garcia, Bruno Ramos-Molina
Summary: Lean/non-obese NAFLD affects a significant portion of the global population and poses a public health concern. Despite similarities in metabolic abnormalities with obese NAFLD, lean NAFLD individuals exhibit differences in genetic predisposition, body composition, gut microbiota, and environmental susceptibility. Current treatment strategies focus on improving overall health and reducing visceral adiposity, with potential pharmacological options being explored for lean NAFLD management.
Article
Medicine, General & Internal
Adnan Khan, Jennifer Pasquier, Vimal Ramachandran, Georgios Ponirakis, Ioannis N. Petropoulos, Omar Chidiac, Binitha Thomas, Amal Robay, Amin Jayyousi, Jassim Al Suwaidi, Arash Rafii, Robert A. Menzies, Talal K. Talal, Seyed Hani Najafi-Shoushtari, Charbel Abi Khalil, Rayaz A. Malik
Summary: Alterations in circulating miRNAs may play an important role in the diagnosis and treatment of diabetic neuropathy. Patients with type 2 diabetes mellitus (T2DM) with corneal nerve loss showed significant differences in neuropathic symptoms, nerve function, and nerve morphology compared to those without nerve loss. Specifically, miR-92b-3p was downregulated while miR-22-3p was upregulated in T2DM patients with corneal nerve loss. Further studies are needed to better understand the role of dysregulated miRNAs in diabetic neuropathy.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Public, Environmental & Occupational Health
Md Marufuzzaman Khan, Shamarial Roberson, Keshia Reid, Melissa Jordan, Agricola Odoi
Summary: The prevalence of stroke is higher among adults with prediabetes and diabetes in Florida. The risk factors for stroke differ between those with prediabetes and those with diabetes, with age, hypertension, and hypercholesterolemia being significant predictors for prediabetes, and non-Hispanic Black race, hypertension, and depression being significant predictors for diabetes.
Article
Biochemistry & Molecular Biology
Chiara Fenoglio, Maria Serpente, Caterina Visconte, Marina Arcaro, Federica Sorrentino, Marianna D'Anca, Andrea Arighi, Emanuela Rotondo, Roberto Vimercati, Giacomina Rossi, Elio Scarpini, Daniela Galimberti
Summary: Frontotemporal Dementia (FTD) is a highly heritable neurodegenerative disorder primarily caused by mutations in MAPT, GRN, and C9ORF72 genes. The role of non-coding RNAs (ncRNAs), including miRNAs and lncRNAs, in FTD pathogenesis is being increasingly recognized. This study examined the expression levels of miRNAs and lncRNAs in FTD patients with different genetic mutations, revealing distinct dysregulation patterns among ncRNAs and specific gene pathways associated with TDP-43 and tau pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Yury O. Nunez Lopez, Anna Casu, Richard E. Pratley
Summary: Given the havoc wreaked by the COVID-19 pandemic, researchers need to implement heightened laboratory safety measures for isolating extracellular vesicles (EVs) from patients to better understand their role in virus infectivity, disease progression, and transmission. Collaborative projects between the EV and virology scientific communities should be developed to address the relationship between endogenous EVs and potentially lethal enveloped viruses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Yiqun Zhang, Lanlan Zhou, Howard Safran, Robyn Borsuk, Rishi Lulla, Nikos Tapinos, Attila A. Seyhan, Wafik S. El-Deiry
Summary: Combining EZH2 inhibitors or HDACi with ONC201 can enhance the effectiveness of treatment on multiple cancer cell lines, possibly through reducing H3K27 methylation. Additionally, synergistic effects were observed in multiple cancer cell lines treated with EPZ-6438 or vorinostat in combination with ONC201, which can promote apoptosis in the cells.
Review
Biochemistry & Molecular Biology
Ilyas Sahin, Andrew George, Attila A. Seyhan
Summary: Comprehensive genomic studies have uncovered various dysregulated RNA processing mechanisms in cancer, such as abnormal mRNA and pre-mRNA processing and pathogenically spliced events, leading to altered protein expression levels and potential therapeutic vulnerabilities. The discovery of these alterations not only offers insights into cancer pathogenesis but also presents novel opportunities for targeted therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Meeting Abstract
Endocrinology & Metabolism
Yury O. Nunez Lopez, Anyon Iliuk, Anna Casu, Daniel S. Lupu, Richard E. Pratley
Article
Biochemistry & Molecular Biology
Yury O. Nunez Lopez, Anton Iliuk, Alejandra M. Petrilli, Carley Glass, Anna Casu, Richard E. Pratley
Summary: This study identified EV proteomic and phosphoproteomic signatures in people with prediabetes and T2DM, offering novel insights into the pathobiology of diabetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Shengliang Zhang, Lindsey Carlsen, Liz Hernandez Borrero, Attila A. Seyhan, Xiaobing Tian, Wafik S. El-Deiry
Summary: This article summarizes the current progress in targeting wild-type and mutant p53 for cancer therapy using biotherapeutic and biopharmaceutical methods. Strategies include boosting p53 activity, restoring p53 pathway function, targeting p53 in immunotherapy, and combination therapies.
Article
Biology
Tatiana D. Saccon, Joseph M. Dhahbi, Augusto Schneider, Yury O. Nunez Lopez, Ahmad Qasem, Marcelo B. Cavalcante, Lauren K. Sing, Saleh A. Naser, Michal M. Masternak
Summary: MicroRNAs are small RNA molecules that play important roles in various diseases, including Crohn's disease and rheumatoid arthritis. Our study identified a large number of differentially expressed miRNAs in the blood of patients with these autoimmune diseases, suggesting their potential as biomarkers. Furthermore, we found that these miRNAs regulate similar biological processes, indicating their possible involvement in disease mechanisms.
Article
Biochemical Research Methods
Bhaswati Sarcar, Bin Fang, Victoria Izumi, Yury O. Nunez Lopez, Alexandra Tassielli, Richard Pratley, Daniel Jeong, Jennifer B. Permuth, John M. Koomen, Jason B. Fleming, Paul A. Stewart
Summary: Small extracellular vesicles (sEVs) released from pancreatic stellate cells (HPSCs) play a role in pancreatic ductal adenocarcinoma (PDAC) progression. This study compares sEVs from normal pancreatic stellate cells (HPaStec) to sEVs from PDAC-associated stellate cells (HPSCs), and identifies differential protein expression between the two. Although sEVs do not directly affect cancer cells, they are efficiently taken up by both normal and cancer epithelial cells. The presence of certain protein markers, such as CSE1L, in HPSC sEVs suggests their significance in poor prognosis of pancreatic cancer patients.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Endocrinology & Metabolism
Yury O. Nunez Lopez, Anna Casu, Zuzana Kovacova, Alejandra M. Petrilli, Olga Sideleva, William G. Tharp, Richard E. Pratley
Summary: This study found that pioglitazone can alter the levels of miRNA in adipocyte-derived extracellular vesicles (EVs) in individuals with diabetes, which in turn affects interorgan crosstalk. The results also showed that these changes are associated with decreased lipolysis and improved insulin sensitivity.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Yury O. Nunez Lopez, Anton Iliuk, Anna Casu, Amay Parikh, Joshua S. Smith, Karen Corbin, Daniel Lupu, Richard E. Pratley
Summary: Recent studies indicate that extracellular vesicles (EVs) are involved in the development of SARS-CoV-2 infection and the severity of COVID-19. However, the connection between COVID-19 and type 2 diabetes (T2D) in relation to EVs has not been explored. This study examined the proteomic and phosphoproteomic profiles of circulating EVs in hospitalized patients with and without T2D, who had either COVID-19 or non-COVID-19 acute respiratory illness (RSP). Differential expression of certain proteins and phosphoproteins was observed, with a central cluster of immunomodulatory and coagulation proteins (C1QA, C1QB, and C1QC) identified in both COVID-19 and T2D networks. The study also revealed the involvement of cellular kinases and EV-shuttled kinases in severe COVID-19, particularly in patients with comorbid diabetes.
DIABETES RESEARCH AND CLINICAL PRACTICE
(2023)
Review
Biochemistry & Molecular Biology
Attila A. Seyhan, Claudio Carini
Summary: Despite the success of immune checkpoint inhibitors (ICI) in cancer treatment, many patients, including those with melanoma, do not benefit long-term from these therapies. There is a need for predictive biomarkers to identify patients who will respond to targeted treatments. Genomic correlations with response and resistance to ICI are being studied to enhance patient outcomes. Emerging genomic signatures, such as neoantigens, antigen presentation, DNA repair, and oncogenic pathways, may play a role in ICI response. Understanding the genomic factors influencing response to ICI will aid in the development of novel biomarkers and strategies to overcome resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alex C. C. Soupir, Yijun Tian, Paul A. A. Stewart, Yury O. O. Nunez-Lopez, Brandon J. J. Manley, Bruna Pellini, Amanda M. M. Bloomer, Jingsong Zhang, Qianxing Mo, Douglas C. C. Marchion, Min Liu, John M. M. Koomen, Erin M. M. Siegel, Liang Wang
Summary: Circulating exosomes in the blood can be used as biomarkers for cancer. This study evaluated the effects of different methods of isolating plasma exosomes on the detection of multi-omic profiles in patients with lung cancer, prostate cancer, and healthy controls. The results showed that different isolation methods enriched distinct exosome cargos, and the size exclusion method captured the most diverse exosome populations. These findings suggest that the choice of isolation method can significantly impact cancer biomarker discovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Attila A. Seyhan
Summary: There is an urgent need for robust and reliable biomarkers for early diagnosis, prognosis, and prediction of response to specific treatments in aggressive and deadly cancers, such as pancreatic cancer. Liquid biopsy-based miRNA profiling shows potential for fulfilling this need. MiRNAs are non-coding RNAs that regulate gene expression and have emerged as potential diagnostic, prognostic, predictive, and therapeutic biomarkers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Alexander G. Raufi, Michael S. May, Matthew J. Hadfield, Attila A. Seyhan, Wafik S. El-Deiry
Summary: Pancreatic cancer is a highly aggressive malignancy with late detection and high recurrence rate. Liquid biopsies represent an emerging group of technologies that can help with diagnosis, prognosis, treatment response prediction, and recurrence detection. Although not yet approved for routine use, the increasing sensitivity and specificity of contemporary liquid biopsy platforms will likely change clinical practice in the near future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Oncology
Alex C. Soupir, Paul A. Stewart, Yury Nunez Lopez, Brandon J. Manley, Bruna Pellini, Jingsong Zhang, Qianxing Mo, Douglas C. Marchion, Min Lui, John M. Koomen, Erin M. Siegel, Liang Wang