Article
Biochemistry & Molecular Biology
Shengping Huang, Larissa L. Dougherty, Prachee Avasthi
Summary: Kinesin-2, composed of KIF3A/3B and KAP3, plays vital roles in cell division and intraorganellar transport, and is highly conserved across species. RanGTP regulates ciliary trafficking of KAP3 and the ARM6-9 region is necessary for both nuclear translocation and ciliary base targeting of KAP3. The precise control of RanGTP levels is crucial for cilium formation and maintenance, with implications for the understanding of nuclear import mechanisms in ciliary import.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Susana Mendez-Gomez, Heidi Espadas-Alvarez, Ivette Ramirez-Rodriguez, Lilianha Dominguez-Malfavon, Refugio Garcia-Villegas
Summary: The localization of TRPV4 ion channel between the nucleus and plasma membrane is crucial for its cellular functions, and mutations may lead to TRPV4 channelopathies.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Cell Biology
Juane Lu, Tao Wu, Biao Zhang, Suke Liu, Wenjun Song, Jianjun Qiao, Haihua Ruan
Summary: This article summarizes the types of nuclear localization signals, focuses on recently reported proteins containing such signals, and briefly outlines mechanisms for nuclear entry that do not rely on nuclear localization signals.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Engineering, Biomedical
Guoxin Tan, Dandan Liu, Renfang Zhu, Hao Pan, Jiayang Li, Weisan Pan
Summary: This study developed a core-shell nanoplatform for targeted delivery of genes to the retina, showing potential for treating retinal diseases such as AMD. The platform demonstrated safety and efficiency in delivering genes to the retina, inhibiting VEGF expression and angiogenesis. This nanoplatform has the capability to overcome barriers in gene delivery to the retina, offering a promising approach for retinal gene therapy.
ACTA BIOMATERIALIA
(2021)
Article
Immunology
Yun Sun, Zhenjie Cao, Panpan Zhang, Caoying Wei, Jianlong Li, Ying Wu, Yongcan Zhou
Summary: This study evaluated the antibacterial effects of Trachinotus ovatus IRF3 (TroIRF3) and its regulation of type I interferon (IFN). It was found that TroIRF3 was highly expressed in the gill and liver and upregulated and transferred to the nucleus after Vibrio harveyi infection. Overexpression of TroIRF3 inhibited bacterial replication, while knocking it down increased replication. Furthermore, overexpression of TroIRF3 increased IFNa3 production and IFN signaling molecules.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Noe Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Recent evidence suggests that nuclear elements play a role in the replication of Flaviviruses, with certain viral proteins being imported into the nucleus. Furthermore, nuclear localization of these proteins has been observed in infected cells. The presence of ZIKV NS3 in the nucleus has been investigated, showing that NS3 is imported into the nucleus via the importin pathway and exported back to the cytoplasm.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yingxue Zhang, Eid Alshammari, Jacob Sobota, Alexander Yang, Chunying Li, Zhe Yang
Summary: SMYD5 is a unique protein lysine methyltransferase with MYND and SET domains. AlphaFold structures are reliable for understanding its structure and function, revealing novel features such as the N-terminal sequence and structural flexibility.
Article
Multidisciplinary Sciences
S. Tsimbalyuk, C. M. Donnelly, J. K. Forwood
Summary: The shuttling of macromolecules between the nucleus and cytoplasm is a regulated process mediated by specific interactions between cargo and nuclear transport proteins. In this study, the crystal structure of human importin alpha 7 was presented, providing insights into the structure-function relationships and isoform specificity within the importin alpha family.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Min Chen, Xue Yang, Haiyang Liu, Jun Wan
Summary: In this study, a functional bipartite nuclear localization signal (bNLS) in the ANKRD11 gene was identified. It was found that this bNLS is responsible for the nuclear import of ANKRD11 and certain clinical variants of this signal may be related to disease development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Fisheries
Wenxing Li, Lulu Ling, Zhixuan Wang, Ying Liang, Wenshu Huang, Pin Nie, Bei Huang
Summary: IRF1 is a member of the interferon regulatory factors family that regulates the expression of interferon-stimulated genes and exhibits functional diversity in different cellular responses. In this study, the IRF1 gene (AjIRF1) was identified in Japanese eel and found to be significantly upregulated in all tested organs/tissues in response to Poly I:C stimulation or Edwardsiella tarda infection. The study also defined the functional motifs required for AjIRF1's nuclear import and its role in activating IFN promoters.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Energy & Fuels
Timur Ildarovich Yunusov, Lyutsia Faritovna Davletshina, Lyubov Abdulaevna Magadova, Mikhail Alexandrovich Silin
Summary: The application of chelating agents in enhanced oil recovery (EOR) and well stimulation is growing rapidly. However, chelating agents have drawbacks, including high interfacial tension (IFT). This paper investigates the interaction between a chelating agent (ethylenediaminetetraacetic acid, EDTA) and anionic and cationic surfactants (sodium dodecyl sulfate, SDS and dodecyl trimethynlammonium bromide, DTAB) through experimental and theoretical methods. The results show that the addition of chelating agents decreases IFT and lowers the critical micelle concentration of surfactants, indicating a salting-out effect. Molecular dynamics simulations reveal closer packing of the surfactant layer and decreased hydration of head groups, with specific attractive interactions observed with SDS. These findings can be useful for selecting appropriate fluids for real-field operations.
Article
Biochemistry & Molecular Biology
Divyanshu Mahajan, Viswanadh Madugula, Lei Lu
Summary: Ar1l3b, an ARF/Arl-family GTPase, is crucial for ciliary organization, trafficking, and signaling. Rab8-GDP and TNPO1 directly bind to the C-terminal stretch of Ar1l3b containing the RVEP motif, and Rab8-GDP enhances the interaction between TNPO1 and Ar1l3b. Mutation of the RVEP motif abolishes the interaction between the C-terminal stretch and Rab8-GDP/TNPO1. Knock-down of Rab8 or TNPO1 decreases the ciliary localization of Ar1l3b.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Cell Biology
Maksim M. M. Karasev, Mikhail Baloban, Vladislav V. V. Verkhusha, Daria M. M. Shcherbakova
Summary: Researchers developed a non-opsin optogenetic tool for light-controlled nuclear export of a protein in neurons. They found that commonly used nuclear localization signals (NLSs) are suboptimal in neurons and identified uncommon NLSs that mediate fast nuclear import. By adjusting the NLSs, they optimized the tool for lower background and higher contrast in neurons.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lihong He, Wei Shao, Jiang Li, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang
Summary: This study systematically analyzed the nuclear localization of 154 ORFs encoded by AcMNPV, with 25 viral proteins found to be localized in the nucleus in the absence of virus infection. Upon virus infection, 11 proteins originally located in the cytoplasm were completely translocated into the nucleus, suggesting their nuclear import is facilitated by other viral or host proteins. Further experiments identified specific proteins that were imported into the nucleus with the assistance of other nuclear localization proteins.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Virology
Margarita A. Kurnaeva, Arthur O. Zalevsky, Eugene A. Arifulin, Olga M. Lisitsyna, Anna Tvorogova, Maria Y. Shubina, Gleb P. Bourenkov, Maria A. Tikhomirova, Daria M. Potashnikova, Anastasia Kachalova, Yana R. Musinova, Andrey Golovin, Yegor S. Vassetzky, Eugene Sheval
Summary: This study investigated the integration of nuclear localization signal (NLS) and nucleolar localization signal (NoLS) into the basic domain of HIV-1 Tat and found that these two supplementary functions are embedded within the amino acid sequence. Integration of NLSs and NoLSs into functional domains of viral proteins enriched with positively charged amino acids allows the concentration of different functions within small protein regions and may have influenced viral evolution by preventing an increase in protein size.
JOURNAL OF VIROLOGY
(2022)
Article
Biology
Hantian Qiu, Sayaka Fujisawa, Shohei Nozaki, Yohei Katoh, Kazuhisa Nakayama
Summary: The binding of INPP5E to ARL13B is crucial for its steady-state localization on the ciliary membrane, but is not necessary for its entry into cilia.
Article
Biology
Sayaka Fujisawa, Hantian Qiu, Shohei Nozaki, Shuhei Chiba, Yohei Katoh, Kazuhisa Nakayama
Summary: INPP5E is localized on the ciliary membrane via its prenyl moiety, with the ARL3 and ARL13B GTPases contributing to maintaining this localization through different mechanisms, with ARL13B mainly determining the ciliary localization of INPP5E through direct binding to it.
Article
Multidisciplinary Sciences
Tatsuro Noguchi, Kentaro Nakamura, Yuuki Satoda, Yohei Katoh, Kazuhisa Nakayama
Summary: CCRK interacts with BROMI to regulate ciliary Hedgehog signaling and both proteins cooperate in regulating ciliary protein trafficking.
Article
Multidisciplinary Sciences
Hantian Qiu, Yuta Tsurumi, Yohei Katoh, Kazuhisa Nakayama
Summary: It has been discovered that pathogenic DYNC2LI1 deletion variants are compromised in their ability to interact with DYNC2H1 and WDR60, and cause ciliary defects when coexpressed with a missense variant that mimics the situation of compound heterozygous ciliopathy cells.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Caroline Shak, Laura Vuolo, Borhan Uddin, Yohei Katoh, Tom Brown, Aakash G. Mukhopadhyay, Kate Heesom, Anthony J. Roberts, Nicola Stevenson, Kazuhisa Nakayama, David J. Stephens
Summary: The primary cilium is a sensory organelle that receives signals from the external environment and transports them into the cell. Mutations in proteins required for transport in the primary cilium result in ciliopathies, which lead to the malformation of organs and skeletal dysplasias. WDR34, a dynein-2 intermediate chain, is necessary for the maintenance of cilia function.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Yuuki Satoda, Tatsuro Noguchi, Taiju Fujii, Aoi Taniguchi, Yohei Katoh, Kazuhisa Nakayama
Summary: Primary cilia are crucial for tissue morphogenesis and contain specific proteins. The study shows that CCRK, BROMI, FAM149B1, and probably CFAP20 together regulate the intraflagellar transport (IFT) machinery and are controlled by ICK. Defects in CCRK, BROMI, and FAM14981 result in abnormally long cilia and accumulation of IFT machinery and ICK at the ciliary tip.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Cell Biology
Zhuang Zhou, Yohei Katoh, Kazuhisa Nakayama
Summary: The intraflagellar transport (IFT) machinery and RABL2 GTPase play important roles in regulating ciliary function. This study reveals the interaction between RABL2 and the IFT-B complex, shedding light on the regulatory mechanisms of ciliary protein trafficking.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Cell Biology
Yamato Ishida, Koshi Tasaki, Yohei Katoh, Kazuhisa Nakayama
Summary: Mutations in IFT52 lead to impaired protein trafficking within cilia, resulting in skeletal ciliopathies.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Cell Biology
Shunya Hiyamizu, Hantian Qiu, Laura Vuolo, Nicola L. Stevenson, Caroline Shak, Kate J. Heesom, Yuki Hamada, Yuta Tsurumi, Shuhei Chiba, Yohei Katoh, David J. Stephens, Kazuhisa Nakayama
Summary: The dynein-2 complex is transported anterogradely within cilia to drive retrograde trafficking of the intraflagellar transport (IFT) machinery containing IFT-A and IFT-B complexes. There are multiple interactions between the dynein-2 and IFT-B subunits, including WDR60 and the DYNC2H1-DYNC2LI1 dimer from dynein-2, and IFT54 and IFT57 from IFT-B. These interactions play a crucial role in the connection between dynein-2 and IFT-B.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biology
Shunya Hiyamizu, Hantian Qiu, Yuta Tsurumi, Yuki Hamada, Yohei Katoh, Kazuhisa Nakayama
Summary: The dynein-2 complex within cilia needs to be transported as an anterograde cargo to enable its function in retrograde trafficking. Previous research has shown that interaction of multiple IFT-B subunits, including IFT54, with WDR60 and the DYNC2H1-DYNC2LI1 dimer of dynein-2 is necessary for the trafficking of dynein-2. However, deleting the IFT54-binding site from WDR60 only has a minor effect on dynein-2 trafficking and function. The results suggest that the C-terminal coiled-coil region of IFT54 is essential for IFT-B function, and the middle linker region of IFT54 is required for ciliary retrograde trafficking by mediating the binding of IFT-B to the dynein-2 complex.
Article
Biochemistry & Molecular Biology
Koshi Tasaki, Zhuang Zhou, Yamato Ishida, Yohei Katoh, Kazuhisa Nakayama
Summary: Due to their important roles in development and homeostasis, defects in cilia lead to ciliopathies with various clinical manifestations. The intraflagellar transport (IFT) machinery, consisting of IFT-A and IFT-B complexes, is responsible for intraciliary trafficking as well as import and export of ciliary proteins. The BBSome, composed of eight subunits encoded by causative genes of Bardet-Biedl syndrome (BBS), links the IFT machinery to ciliary membrane proteins to facilitate their export from cilia. Mutations in IFT-B subunits, including IFT81, can cause skeletal ciliopathies by disrupting the interactions among IFT-B subunits and impairing ciliogenesis and ciliary protein trafficking.
HUMAN MOLECULAR GENETICS
(2023)