Review
Genetics & Heredity
Michael Cargill, Rasika Venkataraman, Stanley Lee
Summary: DEAD-box RNA helicases are crucial regulators of RNA metabolism and are linked to genome stability and cancer development. They play important roles by regulating the expression of factors related to genome stability and repairing DNA damage.
Review
Oncology
Bichun Zheng, Xudong Chen, Qiaoyun Ling, Quan Cheng, Shaoshun Ye
Summary: This review summarizes the role of DEAD-box RNA helicase proteins in CRC and discusses their cellular mechanisms, aiming to provide new strategies for the treatment of CRC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Qi Li, Ningkun Liu, Qing Liu, Xingguo Zheng, Lu Lu, Wenrui Gao, Yang Liu, Yan Liu, Shicheng Zhang, Qian Wang, Jing Pan, Chen Chen, Yingjie Mi, Meiling Yang, Xiaofei Cheng, Guodong Ren, Yao-Wu Yuan, Xiaoming Zhang
Summary: The RNA helicases RH6, RH8, and RH12 play key roles in modulating the formation of membraneless nuclear dicing bodies (D-bodies) in Arabidopsis via liquid-liquid phase separations (LLPSs). Their accumulation decreases in the nuclei upon Turnip mosaic virus infections, leading to a decrease in D-bodies.
Review
Cell Biology
Lu Zhang, Xiaogang Li
Summary: RNA helicases play essential roles in regulating the cell cycle by influencing various cellular processes such as cell proliferation, cell cycle arrest, and apoptosis, impacting multiple diseases. Studies have shown their involvement in regulating progression through different phases of the cell cycle.
Review
Oncology
Massimiliano Secchi, Camilla Lodola, Anna Garbelli, Silvia Bione, Giovanni Maga
Summary: The transformation of a normal cell into a cancerous one is caused by the deregulation of metabolic pathways and protein-protein interactions. Cellular enzymes DDX3X and DDX5 play important roles in normal cell metabolism, but their deregulation can accelerate tumor transformation. This review summarizes the roles of DDX3X and DDX5 in different tumors and discusses their protein-protein interactions in cancer proliferation.
Article
Biochemistry & Molecular Biology
Yongliang Wang, Junwen Liao, Jun Wu, Huimei Huang, Zhanxin Yuan, Wei Yang, Xinying Wu, Xia Li
Summary: In this study, we identified 80 DEAD-box genes in the soybean genome and found that most of these genes were highly conserved in Arabidopsis. However, two genes were specific to soybean. The promoters of these genes contained cis-acting elements involved in plant responses to various stresses, and half of the genes contained nodulation-related elements. Microarray analysis showed differential expression of the DEAD-box genes in roots and nodules, with 31 genes being induced or highly expressed in nodules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Hongjuan You, Lihong Ma, Xing Wang, Fulong Zhang, Yiran Han, Jiaqi Yao, Xiucheng Pan, Kuiyang Zheng, Fanyun Kong, Renxian Tang
Summary: DEAD/H-box helicases are essential proteins that regulate RNA metabolism and innate immune responses. They have been implicated in the development of various diseases, particularly those related to HBV infection. Recent studies have shown that DEAD/H-box helicases interact with HBV, modulating viral replication and innate signaling pathways. Targeting these helicases may be a promising treatment strategy for HBV infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lisa M. Strittmatter, Charlotte Capitanchik, Andrew J. Newman, Martina Hallegger, Christine M. Norman, Sebastian M. Fica, Chris Oubridge, Nicholas M. Luscombe, Jernej Ule, Kiyoshi Nagai
Summary: RNA helicases, such as Prp16 and Prp22, promote correct mRNA synthesis by binding and proofreading on their RNA substrates in defined spliceosome complexes. The stability of spliceosome during exon ligation may affect the proofreading activity of helicases. The new method psiCLIP provides key insights into the binding and proofreading activity of spliceosomal RNA helicases.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Cathal S. Ryan, Martina Schroeder
Summary: The human DEAD-box protein DDX3X plays a crucial role in RNA metabolism and has been associated with various diseases, including cancer and neurodevelopmental conditions. It also plays a significant role in viral infections. DDX3X is involved in translation initiation and stress response, and understanding its regulatory mechanisms is important for developing therapeutic strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Taylor, Agnieszka Piasecka, Arkadiusz Kajdasz, Aleksandra Brzek, Micaela Polay Espinoza, Cyril F. Bourgeois, Artur Jankowski, Malgorzata Borowiak, Katarzyna D. Raczynska, Lukasz J. Sznajder, Krzysztof Sobczak
Summary: This study aims to elucidate the mechanism of muscleblind-like splicing regulators (MBNLs) in alternative splicing (AS) processes. The findings indicate that DEAD-box (DDX) RNA helicases DDX5 and DDX17 play an important role in MBNL-dependent AS regulation. The helicases modulate MBNL splicing activity through changes in the ratio of MBNL splicing isoforms and accessibility of MBNL-binding sites. These findings contribute to a deeper understanding of the interdependencies between RNA-binding proteins and their implications in developmental homeostasis and pathological states.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Microbiology
Ashaq Hussain, Malay Kumar Ray
Summary: Pseudomonas syringae possesses genes for all five major DEAD-box RNA helicases, and disruption of different helicase genes leads to varying effects on the growth of P. syringae mutants at different temperatures. Functional complementation studies show that different helicases have individual and specific roles.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Ashaq Hussain, Malay Kumar Ray
Summary: This study demonstrates the protective role of DEAD box RNA helicases against nucleic acid damaging agents. The results show that different mutants exhibit varying sensitivities to different DNA damaging agents.
INFECTION GENETICS AND EVOLUTION
(2022)
Review
Cell Biology
Shringar Rao, Tokameh Mahmoudi
Summary: This review provides an overview of the functions and mechanisms of DEAD-box family proteins in HIV-1 vRNA metabolism. It discusses the implications of these interactions in viral infection, viral latency, and cell death, and speculates on the potential of DEAD-box protein inhibitors as therapeutics for controlling the HIV-1 pandemic.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Wen Wei, Qiaoli Chen, Minjun Liu, Yang Sheng, Qian OuYang, Weikuan Feng, Xinyu Yang, Longfei Ding, Shu Su, Jingzi Zhang, Lei Fang, Antonio Vidal-Puig, Hong-Yu Wang, Shuai Chen
Summary: The E3-ligase TRIM24 plays a critical role in linking insulin signalling to the P-body molecular machinery. Insulin stimulation triggers the phosphorylation of TRIM24 by protein kinase B, leading to its translocation from the nucleus to the cytoplasm. In the cytoplasm, TRIM24 interacts with P-body components and promotes their polyubiquitylation, facilitating the stabilization of Ppar gamma mRNA. Inactivation of TRIM24 or prevention of its phosphorylation promotes Ppar gamma degradation via P-bodies, alleviating hepatosteatosis in mice fed on a high-fat diet.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Shawn Gray, Wenxiang Cao, Ben Montpetit, Enrique M. De La Cruz
Summary: A kinetic and equilibrium analysis of the Gle1-activated Dbp5 ATPase cycle in Saccharomyces cerevisiae reveals that Gle1 activates Dbp5 by modulating ATP binding and P-i release. These findings provide insights into the roles of Gle1 in regulating Dbp5-mediated RNA export and other gene expression functions.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Abdalla Akef, Eliza S. Lee, Alexander F. Palazzo
Article
Biochemistry & Molecular Biology
Can Cenik, Hon Nian Chua, Guramrit Singh, Abdalla Akef, Michael P. Snyder, Alexander F. Palazzo, Melissa J. Moore, Frederick P. Roth
Review
Biochemistry & Molecular Biology
Alexander F. Palazzo, Abdalla Akef
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2012)
Article
Multidisciplinary Sciences
Eliza S. Lee, Abdalla Akef, Kohila Mahadevan, Alexander F. Palazzo
Article
Biochemistry & Molecular Biology
Kohila Mahadevan, Hui Zhang, Abdalla Akef, Xianying A. Cui, Serge Gueroussov, Can Cenik, Frederick P. Roth, Alexander F. Palazzo
Article
Genetics & Heredity
Can Cenik, Hon Nian Chua, Hui Zhang, Stefan P. Tarnawsky, Abdalla Akef, Adnan Derti, Murat Tasan, Melissa J. Moore, Alexander F. Palazzo, Frederick P. Roth
Article
Cell Biology
Abdalla Akef, Hui Zhang, Seiji Masuda, Alexander F. Palazzo