Article
Entomology
Sai Zhang, Emmanuelle Jacquin-Joly, Nicolas Montagne, Fang Liu, Yang Liu, Guirong Wang
Summary: This study investigated whether odorant receptors (ORs) of the pest moth Spodoptera frugiperda have the capacity to bind type-I sex pheromones and serve as male PRs. Only one OR (SfruOR23) showed a male-biased expression pattern and could bind several type-I sex pheromone compounds. In addition, SfruOR23 also showed weak responses to plant volatile organic compounds. These findings suggest a broader distribution of type-I PRs in moths.
Article
Biochemistry & Molecular Biology
Binu Antony, Jibin Johny, Nicolas Montagne, Emmanuelle Jacquin-Joly, Remi Capoduro, Khasim Cali, Krishna Persaud, Mohammed Ali Al-Saleh, Arnab Pain
Summary: Palm trees hold significant economic, sociocultural, touristic, and heritage value worldwide, with the red palm weevil being a major enemy causing massive economic losses in palm tree cultivation globally. Identifying the pheromone receptor of the red palm weevil opens new avenues for mating disruption and early detection of this pest, potentially offering novel approaches to control this challenging pest.
Review
Biochemistry & Molecular Biology
Jia Li, Long Zhang
Summary: The Asian corn borer is a serious pest that affects corn crops in East Asia. This study provides a summary of the current understanding of the olfactory mechanism in this pest, focusing on sex pheromones and olfactory proteins. It highlights the importance of molecular events in odor detection and transmission and suggests further research to fully comprehend the olfactory activities and functions of these proteins.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Lei Shang, Zi-Cong Li, Ke Tian, Bin Yang, Gui-Rong Wang, Ke-Jian Lin
Summary: In this study, 60 candidate odorant receptors, including eight pheromone receptors, were identified by antennal transcriptome analysis. It was found that GmolOR2 responds to the main sex pheromones Z8-12:OAc and E8-12:OAc, and knockdown of GmolOR2 significantly reduces the males' sensitivity to the main pheromones.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Shuang Shan, Xuan Song, Adel Khashaveh, Shan-Ning Wang, Zi-Yun Lu, Khalid Hussain Dhiloo, Rui-Jun Li, Yong-Jun Zhang
Summary: This study discovered that the parasitoid wasp Microplitis mediator exhibits selective olfactory recognition behavior towards the sex pheromone of Agrotis segetum. Female wasps showed a specific attraction to key components of the host's sex pheromone. These findings have significant implications for enhancing the utilization of natural enemies and pest control.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Wei-Kang Han, Yi-Lin Yang, Yu-Xiao Si, Zhi-Qiang Wei, Si-Ruo Liu, Xiao-Long Liu, Qi Yan, Shuang-Lin Dong
Summary: In this study, the researchers used the CRISPR/Cas9 technique to functionally characterize the role of General odorant-binding protein 2 (GOBP2) in perceiving sex pheromone components in the noctuid pest Spodoptera litura. They found that GOBP2 plays a significant role in sex pheromone perception in both adult and larval stages.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Agronomy
Mei-Mei Wang, Gui-Jun Long, Huan Guo, Xuan-Zheng Liu, Hong Wang, Youssef Dewer, Zhao-Qun Li, Kun Liu, Qiu-Liang Zhang, Yun-Feng Ma, Peng He, Ming He
Summary: In this study, two paralogous antennae-enriched CCEs from Plutella xylostella were identified and functionally characterized, with one enzyme (PxylCCE16c) showing a more essential role in odorant degradation and potential as a novel pesticide target for the notorious moth Plutella xylostella.
PEST MANAGEMENT SCIENCE
(2021)
Article
Entomology
Joerg Fleischer, Alexander Rausch, Kathrin Dietze, Silvio Erler, Sina Cassau, Juergen Krieger
Summary: The study explored the differences in expression of male-biased olfactory receptors in the antennae of European honey bees. It was found that these receptors are expressed in higher numbers in drone bees compared to female workers and queens, indicating a sex-specific difference in antennal cell expression. Further analyses revealed that male-biased receptors are expressed in distinct subsets of olfactory sensory neurons in drones, reminiscent of similar patterns seen in moths for detecting female-released sex pheromones.
Article
Neurosciences
Rebecca E. Roberts, Jothi Kumar Yuvaraj, Martin N. Andersson
Summary: This study explored the potential of codon optimization for insect odorant receptor genes to enhance their expression and functional characterization in human cells, particularly HEK293 cells. While codon optimization improved protein levels and functional characterization for some odorant receptors, it was not universally effective for all receptors tested, suggesting limitations in this approach for studying insect ORs in HEK cells.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Sai Ma, Lu-Lu Li, Wei-Chen Yao, Mao-Zhu Yin, Jian-Qiao Li, Ji-Wei Xu, Youssef Dewer, Xiu-Yun Zhu, Ya-Nan Zhang
Summary: This study found that fourth-instar Spodoptera litura larvae are attracted to cabbage laced with minor sex pheromones, and GOBP2 and OBP7 play a role in this attraction. These findings provide an important basis for exploring the olfactory mechanisms underlying sex pheromone attraction in moth larvae.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Entomology
Sai Zhang, Fang Liu, Bin Yang, Yang Liu, Gui-Rong Wang
Summary: This study characterized the functional properties of pheromone receptors (PRs) in three Spodoptera moth species through genome analysis and heterologous expression. The findings provide a comprehensive understanding of intraspecific communication and interspecific isolation mechanisms in these species.
Article
Multidisciplinary Sciences
Song Cao, Yang Liu, Bing Wang, Guirong Wang
Summary: The study characterized all candidate pheromone receptors of Heliothis subflexa and found that a critical amino acid in OR6 caused a one-way alteration in specificity. This suggests HsubOR6 evolved from an ancestral OR6 gene with a HvirOR6-like function and implies an evolutionary direction of the receptor specificity from a Heliothis virescens-like pattern to a Heliothis subflexa-like pattern.
Article
Agronomy
Yun-Ying Zhang, Teng-Fei Bai, Jin-Meng Guo, Zhi-Qiang Wei, Si-Ruo Liu, Yu He, Jing-Jing Ye, Qi Yan, Jin Zhang, Shuang-Lin Dong
Summary: The study revealed functional differentiations among three PBPs and six PRs in sex pheromone perception in Mythimna loreyi, laying an important basis for understanding the molecular mechanism of sex pheromone perception and developing new control strategies. The findings from electroantennogram assay, fluorescence competition binding assay, and functional study using the Xenopus oocyte system provided insights into the sensitivity of M. loreyi to different pheromone components. Phylogenetic comparison of PRs among different species suggested functional shifts of M. loreyi PRs toward Spodoptera PRs.
PEST MANAGEMENT SCIENCE
(2023)
Article
Physiology
Jiaojiao Luo, Zan Zhang, Dongzhen Li, Jie Liu, Kun Li, Xiao Sun, Lin He
Summary: This study identified that SlitOBP11 in Spodoptera litura larvae may be involved in the perception of female sex pheromones. OBPs in the larvae play an important role in the olfactory perception process.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Jothi K. Yuvaraj, Rebecca E. Roberts, Yonathan Sonntag, Xiao-Qing Hou, Ewald Grosse-Wilde, Ales Machara, Dan-Dan Zhang, Bill S. Hansson, Urban Johanson, Christer Lofstedt, Martin N. Andersson
Summary: This study annotated 73 ORs from an antennal transcriptome of I. typographus and reported the functional characterization of two ORs (ItypOR46 and ItypOR49) responsive to single enantiomers of common bark beetle pheromone compounds. Homology modeling and molecular docking were used to predict their binding sites, providing insight into ligand-OR interaction for the functional evolution of the insect OR gene family. The ecological importance of these receptors and their potential use in pest control and biosensors to detect bark beetle infestations suggest further evaluation of these ORs.
Article
Biochemistry & Molecular Biology
Xiaoqing Hou, Dan-Dan Zhang, Jothi Kumar Yuvaraj, Jacob A. Corcoran, Martin N. Andersson, Christer Lofstedt
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2020)
Article
Entomology
Olle Anderbrant, Jothi Kumar Yuvaraj, Lars-Arne Hogetveit, Anne I. Nissinen, Martin N. Andersson
JOURNAL OF APPLIED ENTOMOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Hui Wen Lee, Prachi Sharma, Bart J. Janssen, Revel S. M. Drummond, Zhiwei Luo, Cyril Hamiaux, Thomas Collier, Jane R. Allison, Richard D. Newcomb, Kimberley C. Snowden
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Physiology
Seung-Joon Ahn, Jacob A. Corcoran, Robert K. Vander Meer, Man-yeon Choi
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Multidisciplinary Sciences
Neil J. Gemmell, Kim Rutherford, Stefan Prost, Marc Tollis, David Winter, J. Robert Macey, David L. Adelson, Alexander Suh, Terry Bertozzi, Jose H. Grau, Chris Organ, Paul P. Gardner, Matthieu Muffato, Mateus Patricio, Konstantinos Billis, Fergal J. Martin, Paul Flicek, Bent Petersen, Lin Kang, Pawel Michalak, Thomas R. Buckley, Melissa Wilson, Yuanyuan Cheng, Hilary Miller, Ryan K. Schott, Melissa D. Jordan, Richard D. Newcomb, Jose Ignacio Arroyo, Nicole Valenzuela, Tim A. Hore, Jaime Renart, Valentina Peona, Claire R. Peart, Vera M. Warmuth, Lu Zeng, R. Daniel Kortschak, Joy M. Raison, Valeria Velasquez Zapata, Zhiqiang Wu, Didac Santesmasses, Marco Mariotti, Roderic Guigo, Shawn M. Rupp, Victoria G. Twort, Nicolas Dussex, Helen Taylor, Hideaki Abe, Donna M. Bond, James M. Paterson, Daniel G. Mulcahy, Vanessa L. Gonzalez, Charles G. Barbieri, Dustin P. DeMeo, Stephan Pabinger, Tracey Van Stijn, Shannon Clarke, Oliver Ryder, Scott Edwards, Steven L. Salzberg, Lindsay Anderson, Nicola Nelson, Clive Stone
Correction
Multidisciplinary Sciences
Neil J. Gemmell, Kim Rutherford, Stefan Prost, Marc Tollis, David Winter, J. Robert Macey, David L. Adelson, Alexander Suh, Terry Bertozzi, Jose H. Grau, Chris Organ, Paul P. Gardner, Matthieu Muffato, Mateus Patricio, Konstantinos Billis, Fergal J. Martin, Paul Flicek, Bent Petersen, Lin Kang, Pawel Michalak, Thomas R. Buckley, Melissa Wilson, Yuanyuan Cheng, Hilary Miller, Ryan K. Schott, Melissa D. Jordan, Richard D. Newcomb, Jose Ignacio Arroyo, Nicole Valenzuela, Tim A. Hore, Jaime Renart, Valentina Peona, Claire R. Peart, Vera M. Warmuth, Lu Zeng, R. Daniel Kortschak, Joy M. Raison, Valeria Velasquez Zapata, Zhiqiang Wu, Didac Santesmasses, Marco Mariotti, Roderic Guigo, Shawn M. Rupp, Victoria G. Twort, Nicolas Dussex, Helen Taylor, Hideaki Abe, Donna M. Bond, James M. Paterson, Daniel G. Mulcahy, Vanessa L. Gonzalez, Charles G. Barbieri, Dustin P. DeMeo, Stephan Pabinger, Tracey Van Stijn, Shannon Clarke, Oliver Ryder, Scott V. Edwards, Steven L. Salzberg, Lindsay Anderson, Nicola Nelson
Article
Multidisciplinary Sciences
Cyril Hamiaux, Colm Carraher, Christer Loefstedt, Jacob A. Corcoran
SCIENTIFIC REPORTS
(2020)
Article
Biology
Jothi K. Yuvaraj, Rebecca E. Roberts, Yonathan Sonntag, Xiao-Qing Hou, Ewald Grosse-Wilde, Ales Machara, Dan-Dan Zhang, Bill S. Hansson, Urban Johanson, Christer Lofstedt, Martin N. Andersson
Summary: This study annotated 73 ORs from an antennal transcriptome of I. typographus and reported the functional characterization of two ORs (ItypOR46 and ItypOR49) responsive to single enantiomers of common bark beetle pheromone compounds. Homology modeling and molecular docking were used to predict their binding sites, providing insight into ligand-OR interaction for the functional evolution of the insect OR gene family. The ecological importance of these receptors and their potential use in pest control and biosensors to detect bark beetle infestations suggest further evaluation of these ORs.
Editorial Material
Biochemistry & Molecular Biology
April A. Jauhal, Richard D. Newcomb
Summary: The study found that while assemblies with high contig and scaffold N50 values tend to have high BUSCO scores, a high BUSCO score can also be achieved in assemblies with low N50 values. N50 is not a perfect proxy for all measures of genome accuracy, and assessing gene space in genome assemblies requires appropriate tools as well as reporting additional genome assessment metrics.
MOLECULAR ECOLOGY RESOURCES
(2021)
Article
Evolutionary Biology
Chen Wu, Victoria G. Twort, Richard D. Newcomb, Thomas R. Buckley
Summary: The study found several meiotic genes duplicated in the New Zealand geographic parthenogenetic stick insect, some of which are rare or unique compared to other arthropods. These genes may play important roles in the evolution of reproductive mode.
GENOME BIOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Jothi Kumar Yuvaraj, Melissa D. Jordan, Dan-Dan Zhang, Martin N. Andersson, Christer Lofstedt, Richard D. Newcomb, Jacob A. Corcoran
Summary: This study investigated the pheromone receptors of the invasive horticultural pest, Epiphyas postvittana. Two clades of receptors were identified, one of which was associated with E. postvittana's sex pheromone blend and the other with sex pheromones from other species.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rebecca E. Roberts, Twinkle Biswas, Jothi Kumar Yuvaraj, Ewald Grosse-Wilde, Daniel Powell, Bill S. Hansson, Christer Lofstedt, Martin N. Andersson
Summary: By investigating the functional properties of odorant receptors (ORs) in different beetle species, this study reveals the evolutionary conservation of OR functions across several beetle species, providing new insights into the functional evolution of insect ORs.
Article
Entomology
Amali H. Thrimawithana, Chen Wu, John T. Christeller, Robert M. Simpson, Elena Hilario, Leah K. Tooman, Doreen Begum, Melissa D. Jordan, Ross Crowhurst, Richard D. Newcomb, Alessandro Grapputo
Summary: This study provides a genomic resource for understanding the biological basis of polyphagy and invasion in the light brown apple moth, a major pest of horticultural plants. The genomic analyses revealed a high proportion of repetitive sequences and expansions in gene families related to its pest status. Population genomics investigation showed likely patterns of invasion and admixture, with Californian moths most likely derived from Australia.
Meeting Abstract
Behavioral Sciences
Sridevi Bhamidipati, Melissa Jordan, Michael Thoma, Amali Thrimawithana, Thomas Buckley, Richard Newcomb