期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 50, 期 -, 页码 77-80出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2014.11.015
关键词
Bipolar electrochemistry; Electrochemiluminescence; Glucose oxidase; Luminol; Prussian blue
资金
- Faculty of Chemistry and Biochemistry at Ruhr-Universitat Bochum
- German Chemical Industry Association e.V. (VCI)
- German National Academic Foundation
The range of potential analytes for bipolar electrochemistry can be significantly extended by modification of bipolar electrodes with enzymatic biosensing layers. In this study, we employed a Prussian blue-based glucose detection system involving electrochemical reduction of enzymatically generated hydrogen peroxide at the cathodic pole. The concentration of glucose in solution can be correlated with oxidative luminol electrochemiluminescence at the opposite anodic pole, which was recorded with a photomultiplier tube. This opens a route for novel analytical systems using glucose oxidase as an amplification element for the reporter reaction. (C) 2014 Elsevier B.V. All rights reserved.
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