期刊
MITOCHONDRION
卷 33, 期 -, 页码 84-101出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2016.08.017
关键词
Proteomics; BK channel; MaxiK channel; Potassium ion channel; Mitochondria; Heart; Tom22; Adenine nucleotide translocator; Macromolecular complex
资金
- National Institutes of Health [HL107418]
Mitochondrial BKca channel, mitoBK(ca), regulates mitochondria function in the heart but information on its protein partnerships in cardiac mitochondria is missing. A directed proteomic approach discovered the novel interaction of BKca with Tom22, a component of the mitochondrion outer membrane import system, and the adenine nucleotide translocator (ANT). The expressed protein partners co-immunoprecipitated and co-segregated into mitochondrial fractions in HEK293T cells. The BKca 50 amino acid splice insert, DEC, facilitated BKca interaction with ANT. Further, BKca transmembrane domain was required for the association with both Tom22 and ANT. The results serve as a working framework to understand mitoBK(ca) import and functional relationships. 2016 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据