Comparison of manual and automatic processing of biological samples for electron microscopy

The ultrastructure of a sample can be observed by electron microscopy (EM), which has become an indispensable research tool in morphological studies. However, EM sample preparation techniques are complicated and time-consuming, with a high labor cost. The current study was conducted to compare the conventional manual and automated methods for sample processing and post-staining for electron microscopy. Automated sample processing reduces OsO4 contamination, improves the efficiency of sample preparation and is easy to use. Therefore, the results of their study provide a practical and feasible method for the preparation of biological samples for electron microscopy.