Article
Microbiology
Kenny Malpartida-Cardenas, Nicolas Moser, Felix Ansah, Ivana Pennisi, Diana Ahu Prah, Linda Eva Amoah, Gordon Awandare, Julius Clemence R. Hafalla, Aubrey Cunnington, Jake Baum, Jesus Rodriguez-Manzano, Pantelis Georgiou
Summary: This study developed seven species-specific loop-mediated isothermal amplification (LAMP) assays for the detection of non-falciparum malaria cases. The clinical performance of these assays was evaluated and compared to other diagnostic methods. The developed molecular diagnostic test showed high sensitivity and specificity for detecting asymptomatic malaria cases.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Xing Geng, Jingyi Chen, Zhaohui Chu, Jingzi Zhang, Fan Zhang, Qingjiang Wang
Summary: A highly sensitive MUC1 assay was developed by integrating MCE with TRA and SDA, enabling quantitative analysis of MUC1. The method showed high sensitivity, high selectivity, simple operation, and low sample consumption. It successfully detected MUC1 in biological samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Engineering, Chemical
Wei-Ting Lin, Su-Chun How, Wen-Zhi Lin, Fang-Hsi Chen, Wan-Chun Liao, I-Cheng Ma, Steven S. -S. Wang, Shao-Yi Hou
Summary: A competitive assay for the detection of biotin using flow cytometry was developed. The assay showed a higher detection limit and shorter assay time compared to ELISA. It involved mixing the sample with streptavidin-display spores, fluorescein-biotin, and additional biotin for competition before analysis with a flow cytometer.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2023)
Article
Chemistry, Analytical
Lin Liu, Daohong Wu, Shuang Zhen, Kaiqing Lu, Xinyao Yi, Zhifang Sun
Summary: This study presents a simple and sensitive electrochemical strategy for detecting telomerase extracted from HeLa cells, utilizing the signal amplification of in-situ formed streptavidin-biotin-DNA-biotin networks. The method effectively amplifies the signal by creating an insulating layer on a gold electrode surface, thus enabling the detection of telomerase.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Marta Broto, Michael M. Kaminski, Christopher Adrianus, Nayoung Kim, Robert Greensmith, Schan Dissanayake-Perera, Alexander J. Schubert, Xiao Tan, Hyemin Kim, Anand S. Dighe, James J. Collins, Molly M. Stevens
Summary: This study combines CRISPR-Cas reaction with nanozyme-linked ELISA to achieve quantitative detection of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). The study shows that CrisprZyme is adaptable to lateral flow reading and different Cas enzymes, and can detect various non-coding RNAs. The platform can be used to identify disease patients and monitor cellular differentiation. CrisprZyme can serve as a universally applicable signal catalyst for CRISPR-based diagnostics, expanding the target range of preamplification-free, quantitative detection.
NATURE NANOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Marjon Zamani, James M. Robson, Andy Fan, Michael S. Bono, Ariel L. Furst, Catherine M. Klapperich
Summary: Sexually transmitted infections, such as HIV and HPV, have a disproportionate impact on low-resource settings. Early diagnosis is crucial for managing HIV and preventing HPV-induced cervical cancer. A novel electrochemical platform combined with loop-mediated isothermal amplification and CRISPR-based detection shows promise for rapidly and cost-effectively detecting viral infections.
ACS CENTRAL SCIENCE
(2021)
Article
Biophysics
Ting Zheng, Xianming Li, Yanjun Si, Minjin Wang, Yuzhen Zhou, Yusheng Yang, Na Liang, Binwu Ying, Peng Wu
Summary: In this study, an accurate point-of-care testing (POCT) platform was proposed by specific recognition of target amplicons with peptide nucleic acid (PNA, assisted by T7 Exonuclease), which efficiently eliminated false positives and showed high sensitivity and specificity for detecting SARS-CoV-2.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Hu Zuo, Xinyang Wang, Wentao Liu, Zongfen Chen, Ruonan Liu, Han Yang, Chunyan Xia, Jinli Xie, Tieqiang Sun, Baoan Ning
Summary: A convenient enzyme-linked immunosorbent assay (MBS-ELISA) based on Avi-labeled nanobody Nb-2G/streptavidin-alkaline phosphatase and magnetic beads was developed for the sensitive detection of OTA. The MBS-ELISA had an IC50 of 1.17 ng/mL and a detection limit value of 0.07 ng/mL, with a linear range of 248.8 pg/mL-5.28 ng/mL. It was almost 20 times more sensitive than the classic BA-ELISA and could generate results within 15 minutes.
Article
Engineering, Electrical & Electronic
Baozhong Zhang, Jintao He, Panpan Tian, Lina Lv, Huina Zhu, Lingling Xie, Xiaolong Liu, Baoshan He
Summary: In this study, an ultrasensitive electrochemical aptasensor was constructed for the detection of PCB77 using nucleic acid aptamers. The sensor demonstrated improved efficiency of electron transfer and enhanced sensitivity through the combination of reduced graphene oxide and Ag-Cu2O. The signal was amplified using streptavidin-modified CeO2/AuPt, and the sensitivity was improved through the SA-biotin system. The sensor provided a high-quality platform for PCB77 detection with a low detection limit and a wide dynamic range.
JOURNAL OF ELECTRONIC MATERIALS
(2022)
Article
Public, Environmental & Occupational Health
N. M. Pollak, G. A. Marsh, M. Olsson, D. McMillan, J. Macdonald
Summary: This study developed a low-resource rapid Hendra virus detection method, which combined fast and simple sample processing with RT-RPA technology and LFD technology. The results were obtained in 30 minutes and only required basic equipment for handling. The test showed high sensitivity and specificity and could provide a safe testing alternative outside of the laboratory.
Article
Chemistry, Analytical
Kira Hallerbach, Khadijeh Khederlou, Lael Wentland, Lana Senten, Steven Brentano, Brian Keefe, Elain Fu
Summary: The use of lateral flow assays for nucleic acid detection has various applications and requires high-density capture surfaces for effective capture. This study characterized several protein-based anchors and demonstrated increased signal gains in target nucleic acid hybridization by increasing the density of capture nucleic acid. It achieved high-sensitivity nucleic acid detection in a lateral flow assay.
Article
Chemistry, Applied
Fanfan Yang, Long Xu, Alberto C. P. Dias, Xiaoying Zhang
Summary: The competitive sandwich immunoassay offers a sensitive and accurate method for detecting histamine in seafood, with a wide linear range and low detection limit.
Article
Chemistry, Physical
Falan Li, Yanfang Wu, Dongfei Chen, Yemin Guo, Xiangyou Wang, Xia Sun
Summary: The study developed a dual-labeled multiple aptasensor for simultaneous detection of multiple antibiotics, using kanamycin and tobramycin as model analytes, with wider detection linear range and lower detection limits compared to previous sensors. The novel sensitive platform demonstrated successful detection of kanamycin and tobramycin spiked in milk samples.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Chemistry, Analytical
Tong Luo, Jiacheng Li, Yao He, Hui Liu, Zhiwei Deng, Xi Long, Qingqing Wan, Jiacheng Ding, Zan Gong, Yanjing Yang, Shian Zhong
Summary: In this study, we developed a novel CRISPR-based biosensor by combining CRISPR/Cas12a with Au-nanobeacon, which allows for direct and rapid detection of nucleic acids in complex biological fluids with high sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Jingjing Qian, Qinming Zhang, Meng Lu
Summary: In recent years, paper-based nucleic acid sensors have been used to detect DNA and RNA molecules extracted from viruses and bacteria. These sensors require the release of nucleic acids from lipid membranes and the removal of interfering molecules for accurate analysis. A portable sensor system has been developed to combine thermolysis and paper-based nucleic acid amplification tests (NAATs) for the detection of viral and exosomal RNA molecules. The system achieved quantitative analysis of influenza A virus (IAV) RNA and exosome microRNA within 1 hour, making it a promising point-of-care test for viral and bacterial infections.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Shuai Wang, Xunyun Huang, Mengyuan Wang, Liangliang Tian, Xiaolei Li, Cong Kong, Feng Han, Xiaoyi Lou, Hongli Ye, Yongfu Shi
Summary: This study reported a simultaneous method for determining PCB101 and its metabolites in aquatic organisms, successfully detecting precursor and metabolites in different aquatic samples. For the first time, the methoxylated metabolite of PCB101 was identified in aquatic organisms, providing support for further research on the environmental properties and biological transmission of PCBs.
ANALYTICAL LETTERS
(2022)
Article
Biochemical Research Methods
Xiaoxia Tian, Yue Dai, Yuanyuan Cheng, Lingdong Zhang, Rong-Mei Kong, Lian Xia, Cong Kong, Guoliang Li
Summary: A PT-SPE method was developed for fast and sensitive detection of plant growth regulators, utilizing a novel COF and PAN to prepare the adsorbent, achieving simultaneous determination of ten PGRs with good analytical performances.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Lei Jiang, Hua-Ying Chen, Cai-Hong He, Han-Bin Xu, Ze-Rui Zhou, Man-Sha Wu, Essy Kouadio Fodjo, Yue He, Mahmoud Elsayed Hafez, Ruo-Can Qian, Da-Wei Li
Summary: Researchers have developed a metal-organic framework (MOF)-based nanoprobe for dual imaging of cellular ATP and ROS, enabling evaluation of cell apoptosis. The nanoprobe combines controlled encapsulation of gold nanorods with a thin zirconium-based MOF layer, and modification with ROS-responsive molecules and ATP aptamer. It allows visualization of ATP and ROS using fluorescence and surface-enhanced Raman spectroscopy, respectively, without mutual interference. This dual-modal assay successfully demonstrates dynamic imaging of ATP and ROS in cancer cells treated with different drugs, revealing their apoptosis-related pathways and interactions in contrast to normal conditions. This study provides a method for studying the relationship between energy metabolism and redox homeostasis in cell apoptosis processes.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Yunyu Tang, Guangxin Yang, Essy Kouadio Fodjo, Shouying Wang, Wenlei Zhai, Wenshuai Si, Lian Xia, Cong Kong
Summary: A novel quantitative method for measuring ENR residues in aquatic products was developed using isotope surrogates. This method reduces the deviation of detected values and allows for direct dilution and re-calibration without repeated sample preparation. The method has a wide linear calibration range and demonstrates high precision in analyzing different ENR residues.
Article
Chemistry, Analytical
Guangxin Yang, Jingru Zhang, Lin Gu, Yunyu Tang, Xuan Zhang, Xuanyun Huang, Xiaosheng Shen, Wenlei Zhai, Essy Kouadio Fodjo, Cong Kong
Summary: A novel ratiometric fluorescence immunoassay was developed for monitoring the illegal use of malachite green (MG) in aquaculture. The method showed a linear ratiometric fluorescence response for MG with a limit of detection of 0.097 mu g center dot kg(-1) and recoveries ranging from 81.88 to 108%. It offers rapid detection, simple sample preparation, and stable signal readout.
Article
Food Science & Technology
Shouying Wang, Guangxin Yang, Yunyu Tang, Yuan Wang, Xiaosheng Shen, Wenshuai Si, Huijuan Yu, Wenlei Zhai, Essy Kouadio Fodjo, Cong Kong
Summary: A quick screening approach was developed for the qualitative and semi-quantitative screening of 87 pesticide residues in aquatic products. The accuracy results showed that most compounds had recoveries ranging from 50 to 120% and relative standard deviations below 20%. Ethoxyquinoline, prometryn, and phoxim were frequently detected in local farmed aquatic products, with trichlorfon and ethoxyquinoline exceeding the safety limits.
Article
Biochemical Research Methods
Yunyu Tang, Guangxin Yang, Yingqing Ma, Dongmei Huang, Wenlei Zhai, Essy Kouadio Fodjo, Xuan Zhang, Siman Li, Weiyi Zhang, Yongfu Shi, Cong Kong
Summary: In this study, a sensitive and validated HPLC-MS/MS method was developed for the determination of moenomycin A in aquatic animal products. The method exhibited good linearity, low detection limit, and was effectively applied in different aquatic samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Li Chen, Yue Guan, Siqing Zheng, Essy Kouadio Fodjo, Wei Deng, Dan Li
Summary: Researchers have developed a new nanosensor that can simultaneously detect hydrogen sulfide (H2S) and sulfur dioxide (SO2) in living cells. The nanosensor utilizes a reaction-mediated dual-recognition strategy to accurately and robustly sense H2S and SO2, providing a valuable tool for studying their roles in physiological processes.
ANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Yunyu Tang, Guangxin Yang, Xiaoxia Liu, Lixia Qin, Wenlei Zhai, Essy Kouadio Fodjo, Xiaosheng Shen, Yuan Wang, Xiaoyi Lou, Cong Kong
Summary: A novel and simplified derivatization method using carbon yarn as a sorbent was developed for the determination of 3-MCPD in soy sauce. The method exhibited high sensitivity and accuracy, making it a preferable alternative for analyzing 3-MCPD contamination in soy sauce.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Jincheng Li, Ruidong Zhou, Guangxin Yang, Essy Kouadio Fodjo, Tengwang Feng, Huiwu Sun, Dongmei Huang, Cong Kong, Huan Liu
Summary: A novel filter-press cleanup column was developed for the rapid screening and quantification of 112 veterinary drugs in fish samples. The method allows single-step cleanup operation and provides high-precision mass information. It has been validated and demonstrated its practicability for multi-residue monitoring veterinary residues for food safety administration.
Article
Multidisciplinary Sciences
Liangliang Tian, Xin Qu, Dongmei Huang, Yongfu Shi, Cong Kong, Yuan Wang
Summary: This research aimed to develop an effective method for detecting semivolatile earthy-musty odors without using conventional sample processing equipment. The method successfully isolated four substances in tap water using a combination of n-hexane liquid-liquid extraction and silica solid-phase extraction techniques. Gas chromatography-mass spectrometry was used for identification, and the method showed good linearity, recovery, and relative standard deviation. The determined limits of detection and quantitation varied for the four substances. The implementation of this method for evaluating off-flavor chemicals in tap water produced satisfactory results.