期刊
MICROCHIMICA ACTA
卷 184, 期 12, 页码 4629-4635出版社
SPRINGER WIEN
DOI: 10.1007/s00604-017-2509-4
关键词
Oxidase-like activity; Peroxidase-like activity; 3,3,5,5-tetramethylbenzidine; Dual enzyme; Nanozyme
资金
- National Natural Science Foundation of China [21365005]
- Guangxi Natural Science Foundation of China [2014GXNSFGA118002]
- BAGUI Scholar Program
A bimetallic metal-organic framework of the type MOF(Co/2Fe), synthesized by a hydrothermal method, is demonstrated to exhibit peroxidase-like activity and oxidase-like activity. The material catalyzes the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) to produce a blue product both in the absence and presence of H2O2. Its catalytic activity strongly depends on temperature and the solution pH value. With its dual enzyme mimetic activity, MOF(Co/2Fe) has the advantages of low cost, good stability, ease of preparation, and attractive MichaelisMenten behavior. The mechanisms and kinetics of the pseudo-enzymatic activity were studied in some detail. A colorimetric method was developed for determination of H2O2 that is based on the peroxidase-like activity of MOF(Co/2Fe). Under the optimal conditions, the absorbance of oxidized TMB at 652 nm increases linearly in the 10 to 100 mu M H2O2 concentration range, with a 5 mu M limit of detection.
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