4.2 Article

The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2)

期刊

MICROBIOLOGY-SGM
卷 163, 期 10, 页码 1415-1419

出版社

MICROBIOLOGY SOC
DOI: 10.1099/mic.0.000524

关键词

Streptomyces; antibiotics; sporulation; cryptic gene clusters

资金

  1. BBSRC
  2. UEA
  3. Medical Research Council [G0801721]
  4. Natural Environment Research Council [NE/M015033/1, NE/M014657/1]
  5. BBSRC [BBS/E/J/000PR9790, BBS/E/J/000PR9791, BB/P005292/1, BBS/E/J/000C0665, BBS/E/J/000CA589] Funding Source: UKRI
  6. NERC [NE/M015033/1, NE/M014657/1] Funding Source: UKRI
  7. Biotechnology and Biological Sciences Research Council [BB/P005292/1, BBS/E/J/000PR9791, BBS/E/J/000C0665, BBS/E/J/000PR9790, BBS/E/J/000CA589] Funding Source: researchfish
  8. Medical Research Council [G0801721] Funding Source: researchfish
  9. Natural Environment Research Council [NE/M015033/1, NE/M014657/1] Funding Source: researchfish

向作者/读者索取更多资源

MtrAB is a highly conserved two-component system implicated in the regulation of cell division in the Actinobacteria. It coordinates DNA replication with cell division in the unicellular Mycobacterium tuberculosis and links antibiotic production to sporulation in the filamentous Streptomyces venezuelae. Chloramphenicol biosynthesis is directly regulated by MtrA in S. venezuelae and deletion of mtrB constitutively activates MtrA and results in constitutive over-production of chloramphenicol. Here we report that in Streptomyces coelicolor, MtrA binds to sites upstream of developmental genes and the genes encoding ActII-1, ActII-4 and RedZ, which are cluster-situated regulators of the antibiotics actinorhodin (Act) and undecylprodigiosin (Red). Consistent with this, deletion of mtrB switches on the production of Act, Red and streptorubin B, a product of the Red pathway. Thus, we propose that MtrA is a key regulator that links antibiotic production to development and can be used to upregulate antibiotic production in distantly related streptomycetes.

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