期刊
MICROBIOLOGY
卷 86, 期 3, 页码 310-316出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0026261717030080
关键词
Escherichia coli; spontaneous mutants; bacteriophage sensitivity; lipopolysaccharide; O antigen; polymyxin; lysozyme; outer membrane permeability; O antigen acetylation
类别
资金
- Russian Science Foundation [15-15-00134]
- Russian Science Foundation [15-15-00134] Funding Source: Russian Science Foundation
Comparison of the methods for determination of permeability of the outer membrane of Escherichia coli strain 4s and its mutants was carried out. The studied isogenic strains E. coli 4s were obtained by selection of spontaneous mutants according to their sensitivity to bacteriophages recognizing the surface O antigen of the outer membrane lipopolysaccharide as a primary receptor. The variants differed in the presence and (de)acetylation of the lipopolysaccharide O antigen. A peptide antibiotic polymyxin, plasmid DNA, and lysozyme were used as probes. The role of acetylation of the O antigen of the lipopolysaccaride of E. coli outer membrane in modification of its permeability (correlating with bacteriophage sensitivity of the cells) was confirmed. Kinetic analysis using lysozyme was shown to be the optimal method for determination of the barrier function of E. coli outer membrane.
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