The Use of Degenerate Primers in qPCR Analysis of Functional Genes Can Cause Dramatic Quantification Bias as Revealed by Investigation of nifH Primer Performance

Simple Absolute Quantification Method Correcting for Quantitative PCR Efficiency Variations for Microbial Community Samples

(2012) Robert Brankatschk et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase

(2012) John Christian Gaby et al.   PLoS One

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers

(2011) Ingrid Balcells et al.   BMC BIOTECHNOLOGY

A global census of nitrogenase diversity

(2011) John Christian Gaby et al.   ENVIRONMENTAL MICROBIOLOGY

Differences in amplification efficiency of standard curves in quantitative real-time PCR assays and consequences for gene quantification in environmental samples

(2010) Stefanie Töwe et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Unicellular Cyanobacterial Distributions Broaden the Oceanic N2 Fixation Domain

(2010) P. H. Moisander et al.   SCIENCE

Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example

(2008) D. Bru et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Critical Evaluation of Two Primers Commonly Used for Amplification of Bacterial 16S rRNA Genes

(2008) J. A. Frank et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Evaluation of PCR primers for universal nifH gene targeting and for assessment of transcribed nifH pools in roots of Oryza longistaminata with and without low nitrogen input

(2008) Moudjahidou Demba Diallo et al.   FEMS MICROBIOLOGY ECOLOGY

IDT SciTools: a suite for analysis and design of nucleic acid oligomers

(2008) R. Owczarzy et al.   NUCLEIC ACIDS RESEARCH