Article
Biochemistry & Molecular Biology
Maria Joao Pena, Raquel Costa, Ilda Rodrigues, Sandra Martins, Joao Tiago Guimaraes, Ana Faria, Conceicao Calhau, Julio Cesar Rocha, Nuno Borges
Summary: This study compared the metabolic effects of casein glycomacropeptide (GMP) and free amino acids (fAA) in rats, revealing differences in body weight, insulin levels, and GLP-1 production between GMP and fAA. The chronic consumption of different diets triggered varied metabolic responses, providing clues for further investigations into potential underlying mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Marc-Olivier Deguise, Lucia Chehade, Rashmi Kothary
Summary: Spinal muscular atrophy (SMA) is a genetic disorder causing paralysis, muscle atrophy, and death. Recent research suggests that gastrointestinal, metabolic, and endocrine defects may contribute to the disease phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Livingstone Fultang, Luciana Gneo, Carmela De Santo, Francis J. Mussai
Summary: This article reviews the changes in expression patterns of amino acid metabolic enzymes and transporters in myeloid malignancies, as well as the increasing list of small molecules and therapeutic strategies used to disrupt amino acid metabolic circuits within the cells. Pharmacological inhibition of amino acid metabolism effectively induces cell death in leukemic stem cells and primary blasts, and reduces tumor burden in in vivo murine models of human diseases. Targeting amino acid metabolism offers a range of translational opportunities for improving outcomes in patients with myeloid malignancies.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Jacopo Di Gregorio, Sabrina Petricca, Roberto Iorio, Elena Toniato, Vincenzo Flati
Summary: Metabolic alterations, including enhanced aerobic glycolysis, oxidative phosphorylation, gluconeogenesis, and fatty acid beta-oxidation, play crucial roles in the development and survival of cancers.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Arnaud Germain, Ludovic Giloteaux, Geoffrey E. Moore, Susan M. Levine, John K. Chia, Betsy A. Keller, Jared Stevens, Carl J. Franconi, Xiangling Mao, Dikoma C. Shungu, Andrew Grimson, Maureen R. Hanson
Summary: Post-exertional malaise (PEM) is a prominent symptom of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). By monitoring plasma metabolites, this study found significant differences in metabolic response and recovery pattern between ME/CFS patients and healthy control participants after two maximal exercise tests. Glutamate metabolism was identified as a crucial factor in the metabolic disruptions that lead to PEM.
Review
Oncology
Ahmad Ali, Ugo Chianese, Chiara Papulino, Antonella Toraldo, Mawada Elmagboul Abdalla Abakar, Eugenia Passaro, Rosario Cennamo, Nunzio Del Gaudio, Lucia Altucci, Rosaria Benedetti
Summary: This article describes the metabolic features of pancreatic ductal adenocarcinoma and discusses how this could be exploited as a weakness for clinical interventions. Metabolism plays a crucial role in the development of PDAC, and interventions on bioenergetic circuits could potentially reduce its aggressiveness.
Article
Agriculture, Dairy & Animal Science
T. L. Chandler, T. A. Westhoff, T. R. Overton, A. L. Lock, M. E. Van Amburgh, A. S. Sipka, S. Mann
Summary: This study investigated the response to systemic inflammation in postpartum cows after intravenous amino acid infusion. The results showed that amino acid infusion changed the metabolic status and milk fat content in cows, but did not appear to alter the metabolic response to subsequent systemic inflammation.
JOURNAL OF DAIRY SCIENCE
(2022)
Review
Endocrinology & Metabolism
Sarah L. Armour, Jade E. Stanley, James Cantley, E. Danielle Dean, Jakob G. Knudsen
Summary: Glucagon and its producing cells, pancreatic islet alpha cells, have remained enigmatic in relation to insulin-producing beta cells. However, recent data suggest a more holistic model of metabolic signaling, involving the regulation of multiple nutrients and reciprocal feedback to regulate glucagon secretion and alpha cell mass.
JOURNAL OF ENDOCRINOLOGY
(2023)
Article
Food Science & Technology
Clemence Rives, Celine Marie Pauline Martin, Lauris Evariste, Arnaud Polizzi, Marine Huillet, Frederic Lasserre, Valerie Alquier-Bacquie, Prunelle Perrier, Jelskey Gomez, Yannick Lippi, Claire Naylies, Thierry Levade, Frederique Sabourdy, Herve Remignon, Pierre Fafournoux, Benoit Chassaing, Nicolas Loiseau, Herve Guillou, Sandrine Ellero-Simatos, Laurence Gamet-Payrastre, Anne Fougerat
Summary: This study found that dietary intake of free amino acids can prevent the unhealthy metabolic outcomes of a Western diet in male mice. Replacing casein with a free amino acid mixture that mimics its composition prevents weight gain in both male and female mice. Free amino acids in the diet can prevent liver damage and associated changes in liver gene expression only in males. These results indicate that dietary intake of free amino acids can prevent the negative metabolic effects of a Western diet in a sex-specific manner, possibly involving the gut microbiota.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Endocrinology & Metabolism
Mary-Margaret E. Remchak, Emily M. Heiston, Anna Ballantyne, Brielle L. Dotson, Nathan R. Stewart, Andrea M. Spaeth, Steven K. Malin
Summary: Early chronotype is associated with higher insulin sensitivity and metabolic flexibility, which is related to plasma TCA concentration.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Review
Medicine, Research & Experimental
Dong Wang, Xin Wan
Summary: This paper reviews the effects of amino acid metabolism on tumor cell proliferation, analyzes the abnormal changes in amino acid metabolism during the tumor metabolic cycle, and prospects tumor therapeutic drugs targeting amino acid metabolism.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Agriculture, Dairy & Animal Science
Guilherme Henrique Tamarindo, Adriana Alonso Novais, Luiz Gustavo Almeida Chuffa, Debora Aparecida Pires Campos Zuccari
Summary: Metabolic reprogramming in canine mammary tumors presents a therapeutic opportunity. Alterations in carbohydrate metabolism, amino acid metabolism, lipid metabolism, and mitochondrial functions have been observed in these tumors. Targeting metabolic vulnerabilities may improve the outcome of canine mammary tumors.
Article
Biochemistry & Molecular Biology
Teresa Diaz Calvo, Noemi Tejera, Iain McNamara, Gemma C. Langridge, John Wain, Mark Poolman, Dipali Singh
Summary: In this study, a fully curated, genome-scale metabolic model for S. epidermidis RP62A was constructed and its metabolic properties were investigated. The results showed that the strain preferred amino acids, especially proline, valine, alanine, glutamate, and arginine, for energy and biomass production. Unlike previous studies, this strain did not show significant substrate auxotrophies, although removal of proline from the media had the highest impact on growth characteristics.
Article
Endocrinology & Metabolism
Deirdre K. Tobias, Samia Mora, Subodh Verma, Filio Billia, Julie E. Buring, Patrick R. Lawler
Summary: The study found that fasting was associated with lower plasma BCAA levels in women without metabolic syndrome or T2D, but had no significant impact on women with Type 2 diabetes. These results suggest that impaired BCAA metabolism may be related to the pathophysiology of T2D.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Article
Plant Sciences
Feng Zhou, Xu-Yang Liu, Lin -Lin Liu, Yan Hou, Zisheng Han, Liang Zhang
Summary: By conducting a metabolomics analysis, sensory evaluation, and transcriptomic analysis on tea leaves with and without mistletoe, it was found that mistletoe affects the secondary metabolites and gene expression in tea leaves, leading to a decrease in catechin content but an increase in free amino acid content.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Carlos Pena-Varas, Christa Kanstrup, Ariela Vergara-Jaque, Mariela Gonzalez-Avendano, Christoph Crocoll, Osman Mirza, Ingo Dreyer, Hussam Nour-Eldin, David Ramirez
Summary: In this study, computational simulation approaches were used to investigate how the plant glucosinolate transporter GTR1 transports specific substrates. By docking and analyzing a range of GTR1 conformations, key residues essential for substrate transport were identified. The incorporation of structural bioinformatics accelerated the understanding of plant transporter functions and optimized the use of time and resources.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
David A. Russo, Julie A. Z. Zedler, Fabian D. Conradi, Nils Schuergers, Poul Erik Jensen, Conrad W. Mullineaux, Annegret Wilde, Georg Pohnert
Summary: Cyanobacteria interact with the environment through the secretion of various molecules and proteins mediated by multiprotein complexes. The type IVa pilus system (T4aPS) in cyanobacteria plays a crucial role in dynamic cell surface appendages assembly. The development of a NanoLuc (NLuc)-based quantitative secretion reporter provides a highly sensitive tool to study protein secretion systems in cyanobacteria, leading to new insights into secretion mechanisms and potential applications in biotechnology.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Genetics & Heredity
Max Cowan, Birger Lindberg Moller, Sally Norton, Camilla Knudsen, Christoph Crocoll, Agnelo Furtado, Robert Henry, Cecilia Blomstedt, Roslyn M. Gleadow
Summary: Domestication has caused a loss of genetic diversity in major food crops, but crop wild relatives could potentially provide novel genes for regaining climate resilience. Australian Sorghum wild species were found to be phenotypically acyanogenic, possibly due to their nutrient-poor environments. The transcriptional regulation of cyanogenic phenotype in wild sorghum is similar to that in elite sorghum.
Review
Agronomy
Lars B. Scharff, Vandasue L. R. Saltenis, Poul Erik Jensen, Alexandra Baekelandt, Alexandra J. Burgess, Meike Burow, Aldo Ceriotti, Jean-Pierre Cohan, Fernando Geu-Flores, Barbara Ann Halkier, Richard P. Haslam, Dirk Inze, Rene Klein Lankhorst, Erik H. Murchie, Johnathan A. Napier, Philippe Nacry, Martin A. J. Parry, Angelo Santino, Aurelia Scarano, Francesca Sparvoli, Ralf Wilhelm, Mathias Pribil
Summary: Enhancing the nutritional quality of crops through increasing protein content, improving protein functionality, and optimizing the content of vitamins and minerals, while reducing antinutrients and toxins, could help address 'hidden hunger' caused by micronutrient deficiencies and support a shift towards healthier and more sustainable plant-based diets.
FOOD AND ENERGY SECURITY
(2022)
Article
Food Science & Technology
Carmen Masia, Poul Erik Jensen, Iben Lykke Petersen, Patrizia Buldo
Summary: This study investigates the stability and gel formation of pea protein matrices for fermentation-induced plant-based cheese. The optimal pea protein matrix for fermentation-induced pea protein gels can be produced with 10% protein content and 10% olive oil levels without compromising gel hardness.
Article
Engineering, Environmental
Chen Wang, Hans Christian Bruun Hansen, Mogens Larsen Andersen, Bjarne W. Strobel, Hui Ma, Nadia Dodge, Poul Erik Jensen, Changyong Lu, Peter E. Holm
Summary: This study investigates the oxidation of the antibiotic norfloxacin (NOR) using cyanobacterial nitrogen rich biochars (CBs) as catalysts for peroxydisulfate (PDS). The results show that CB950, pyrolyzed at 950 degrees C, is highly effective in degrading NOR, with a faster degradation rate compared to low pyrolysis temperature (PT) CBs. The high pyrolysis temperature CBs also exhibit full reactivity after repeated uses and high defluorination and mineralization rates. The study suggests that high pyrolysis temperature biochars from algal bloom biomass can be used as catalysts for organic contaminant oxidation.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Christa Kanstrup, Claire C. Jimidar, Josip Tomas, Giuliano Cutolo, Christoph Crocoll, Marie Schuler, Philipp Klahn, Arnaud Tatibouet, Hussam Hassan Nour-Eldin
Summary: Glucosinolate transporters (GTR1/2/3) from the NPF family are crucial for the transport, accumulation, and distribution of glucosinolates. By synthesizing fluorescent glucosinolates, the ability of GTR1/2/3 from Arabidopsis thaliana to import these compounds was investigated. Five out of seven fluorescent glucosinolates were successfully imported by at least one GTR. The uptake mechanism of fluorescent glucosinolates was found to be similar to that of natural glucosinolates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Carmen Masia, Shaghayegh Keshanidokht, Laerke Due Preisler, Jens Risbo, Poul Erik Jensen
Summary: Lipids can affect cheese texture and quality. This study evaluated the effect of different lipid sources, such as sunflower oil and coconut oil, on emulsion stability and gel structure in fermented pea protein gels compared to dairy cheese. The results showed that the lipid sources did not significantly affect emulsion stability, but the dairy cheese was harder than the pea protein gels. However, the rheological properties of the pea protein gels with oil, oleosomes, and oleogel were similar to the dairy cheese.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Deyang Xu, Niels Christian Holm Sanden, Line Lykke Hansen, Zeinu Mussa Belew, Svend Roesen Madsen, Lasse Meyer, Morten Egevang Jorgensen, Pascal Hunziker, Dorottya Veres, Christoph Crocoll, Alexander Schulz, Hussam Hassan Nour-Eldin, Barbara Ann Halkier
Summary: Plant membrane transporters control metabolite distribution, contributing to key agronomic traits. Mutations in importers can prevent the accumulation of anti-nutritional factors in edible parts, but may alter the distribution pattern within the plant. Engineering of exporters can prevent these changes while still improving the nutritional quality of the seeds.
Article
Biochemistry & Molecular Biology
Pablo D. Cardenas, Jonas P. Landtved, Signe H. Larsen, Nicolai Lindegaard, Sebastian Wohlk, Karen R. Jensen, David I. Pattison, Meike Burow, Soren Bak, Christoph Crocoll, Niels Agerbirk
Summary: This study investigated the production of phytoalexins in the crucifer plant Barbarea vulgaris after exposure to abiotic stress. The results showed that two genotypes of B. vulgaris accumulated three major phytoalexins in the treated leaves. The levels of phytoalexins varied among plant types and individual phytoalexins. Summary: Two genotypes of B. vulgaris accumulated three major phytoalexins in response to abiotic stress.
Article
Microbiology
Thiyagarajan Gnanasekaran, Arjun Sarathi, Qing Fang, Asieh Azarm, Juliana Assis Geraldo, Eleonora Nigro, Manimozhiyan Arumugam
Summary: The quantitative composition of synthetic gut microbial communities (SGMCs) does not significantly impact the eventual stable microbial community. Regardless of the nutrient media used, the microbiome compositions of fecal and equal SGMC inoculums converge before reaching stable community compositions. This has important implications for simplifying in vitro SGMC investigations.
Review
Food Science & Technology
Ourania Gouseti, Mads Emil Larsen, Ashwitha Amin, Serafim Bakalis, Iben Lykke Petersen, Rene Lametsch, Poul Erik Jensen
Summary: With the growth of the plant-based food market, there is an increasing demand for plant protein. Seed storage proteins, which are abundant in legumes and oilseeds, have great potential for use in plant-based food development. However, they often have low functionality due to their natural design, and there are challenges related to knowledge gap, high enzyme cost, and complexity of plant proteins.
Article
Food Science & Technology
Carmen Masia, Raquel Fernandez-Varela, Poul Erik Jensen, Saeed Rahimi Yazdi
Summary: Texture and flavour development in plant-based cheese can be improved through fermentation, which can help to remove off-flavours and develop dairy-like flavours.
Article
Geriatrics & Gerontology
A. Augusto Peluso, Agnete T. Lundgaard, Parizad Babaei, Felippe Mousovich-Neto, Andrea L. Rocha, Mads V. Damgaard, Emilie G. Bak, Thiyagarajan Gnanasekaran, Ole L. Dollerup, Samuel A. J. Trammell, Thomas S. Nielsen, Timo Kern, Caroline B. Abild, Karolina Sulek, Tao Ma, Zach Gerhart-Hines, Matthew P. Gillum, Manimozhiyan Arumugam, Cathrine Orskov, Douglas McCloskey, Niels Jessen, Markus J. Herrgard, Marcelo A. S. Mori, Jonas T. Treebak
Summary: The gut microbiota is influenced by dietary supplementation of nicotinamide riboside (NR), an NAD(+) precursor. NR altered the gut microbiota in rats and mice, leading to changes in fat and energy absorption. However, the gut microbiota of humans remained unaltered by NR supplementation. These findings suggest that the effects of NR on the gut microbiota may be species-specific.
Article
Microbiology
Carmen Saenz, Qing Fang, Thiyagarajan Gnanasekaran, Samuel Addison Jack Trammell, Jesse Arnold Buijink, Paola Pisano, Michael Wierer, Frederic Moens, Bettina Lengger, Asker Brejnrod, Manimozhiyan Arumugam
Summary: Clostridioides difficile infection (CDI) is a major health concern and a leading cause of hospital-acquired diarrhea. Conventional treatment with broad-spectrum antibiotics often leads to recurrent CDI, highlighting the need for alternative solutions. Recent studies have shown that microbiota-based therapeutics, such as bile acids, hold promise in treating CDI.
MICROBIOLOGY SPECTRUM
(2023)