Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies

Systematic testing of enzyme perturbation sensitivities via graded dCas9 modulation in Saccharomyces cerevisiae

(2017) Matthew Deaner et al.   METABOLIC ENGINEERING

SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

(2017) Katherina García Vanegas et al.   Microbial Cell Factories

Overexpression of OLE1 enhances cytoplasmic membrane stability and confers resistance to cadmium in Saccharomyces cerevisiae

(2016) Zhijia Fang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes intoSaccharomyces cerevisiaevia CRISPR-Cas9

(2016) Mathew M Jessop-Fabre et al.   Biotechnology Journal

CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production

(2016) Seong Keun Kim et al.   METABOLIC ENGINEERING

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

(2016) Benjamin L Oakes et al.   NATURE BIOTECHNOLOGY

Multidimensional chemical control of CRISPR–Cas9

(2016) Basudeb Maji et al.   Nature Chemical Biology

Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR–Cas9-Mediated Genome Editing in Yarrowia lipolytica

(2016) Cory M. Schwartz et al.   ACS Synthetic Biology

EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes intoSaccharomyces cerevisiaevia CRISPR-Cas9

(2016) Mathew M Jessop-Fabre et al.   Biotechnology Journal

Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

(2016) R Stefan Isaac et al.   eLife

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

(2016) Max A Horlbeck et al.   eLife

Impact of synthetic biology and metabolic engineering on industrial production of fine chemicals

(2015) David Jullesson et al.   BIOTECHNOLOGY ADVANCES

Metabolic engineering of Saccharomyces cerevisiae for linalool production

(2015) Pegah Amiri et al.   BIOTECHNOLOGY LETTERS

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Quantitative Analysis of Proteome and Lipidome Dynamics Reveals Functional Regulation of Global Lipid Metabolism

(2015) Albert Casanovas et al.   CHEMISTRY & BIOLOGY

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

(2015) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Principal component analysis of proteomics (PCAP) as a tool to direct metabolic engineering

(2015) Jorge Alonso-Gutierrez et al.   METABOLIC ENGINEERING

Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica

(2015) Kangjian Qiao et al.   METABOLIC ENGINEERING

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

(2015) Yaojun Tong et al.   ACS Synthetic Biology

CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae

(2015) Tadas Jakočiu̅nas et al.   ACS Synthetic Biology

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

(2014) Tessa G. Montague et al.   NUCLEIC ACIDS RESEARCH

Improving Production of Malonyl Coenzyme A-Derived Metabolites by Abolishing Snf1-Dependent Regulation of Acc1

(2014) S. Shi et al.   mBio

Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development

(2014) Alice Pajoro et al.   GENOME BIOLOGY

Rapid Quantification of Yeast Lipid using Microwave-Assisted Total Lipid Extraction and HPLC-CAD

(2013) Sakda Khoomrung et al.   ANALYTICAL CHEMISTRY

From flavors and pharmaceuticals to advanced biofuels: Production of isoprenoids inSaccharomyces cerevisiae

(2013) Stefan Tippmann et al.   Biotechnology Journal

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals

(2013) Weerawat Runguphan et al.   METABOLIC ENGINEERING

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

CRISPR RNA–guided activation of endogenous human genes

(2013) Morgan L Maeder et al.   NATURE METHODS

Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay

(2013) Michael E. Lee et al.   NUCLEIC ACIDS RESEARCH

Transcriptional Networks Controlling the Cell Cycle

(2013) Martin Bonke et al.   G3-Genes Genomes Genetics

Dynamic control of gene expression in Saccharomyces cerevisiae engineered for the production of plant sesquitepene α-santalene in a fed-batch mode

(2012) Gionata Scalcinati et al.   METABOLIC ENGINEERING

Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae

(2012) Gionata Scalcinati et al.   Microbial Cell Factories

Harnessing yeast subcellular compartments for the production of plant terpenoids

(2011) Moran Farhi et al.   METABOLIC ENGINEERING

A Comprehensive Genomic Binding Map of Gene and Chromatin Regulatory Proteins in Saccharomyces

(2011) Bryan J. Venters et al.   MOLECULAR CELL

Large-scale 13C-flux analysis reveals distinct transcriptional control of respiratory and fermentative metabolism in Escherichia coli

(2011) B. R. B. Haverkorn van Rijsewijk et al.   Molecular Systems Biology

Lipidomics: coming to grips with lipid diversity

(2010) Andrej Shevchenko et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae

(2010) Siavash Partow et al.   YEAST

The Yap family and its role in stress response

(2010) Claudina Rodrigues-Pousada et al.   YEAST

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

The DNA-encoded nucleosome organization of a eukaryotic genome

(2008) Noam Kaplan et al.   NATURE