Article
Microbiology
Damien M. R. Rivers, Derek D. Kim, Ivan J. Oresnik
Summary: This study demonstrates that Rhizobium leguminosarum strains unable to utilize rhamnose as a sole carbon source are less competitive for nodule occupancy. Through a series of experiments and analyses, the researchers show that the regulator RhaR in Sinorhizobium meliloti is dominant to its counterpart in R. leguminosarum, and that rhamnose catabolism is a general determinant in competition for nodule occupancy among rhizobial species.
Article
Multidisciplinary Sciences
Barney A. Geddes, Jason V. S. Kearsley, Jiarui Huang, Maryam Zamani, Zahed Muhammed, Leah Sather, Aakanx K. Panchal, George C. diCenzo, Turlough M. Finan
Summary: The symbiosis between leguminous plants and rhizobia in root nodules plays a key role in sustainable agricultural systems. Research has identified a minimal bacterial gene complement necessary for successful symbiosis and nitrogen fixation, paving the way for engineering symbiosis in nonlegume crops. By reorganizing the minimal symbiotic genes into three modules, researchers have provided a platform for expanding symbiosis to other plant partners and identifying genetic components that modulate competitiveness for nodulation and the effectiveness of rhizobia-plant symbioses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Agronomy
Emmanuelle D'Amours, Annick Bertrand, Jean Cloutier, Annie Claessens, Solen Rocher, Philippe Seguin
Summary: This study reveals a relationship between nodule and shoot regrowth after freezing stress, with a higher proportion of undamaged nodules related to increased shoot biomass. The results demonstrate that choosing stress-adapted alfalfa populations and S. meliloti strains can enhance alfalfa persistence.
Article
Plant Sciences
Marie-Francoise Jardinaud, Sebastien Carrere, Benjamin Gourion, Pascal Gamas
Summary: Symbiotic nitrogen fixation plays a key role in reducing the negative impact of nitrogen fertilizers in agroecosystems. The efficiency of this process is influenced by the combination of bacterial and plant genotypes, but the mechanisms behind differences in the efficiency of rhizobium strains are not well known.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Microbiology
Natalia I. Garcia-Tomsig, Fernando M. Garcia-Rodriguez, Sabina K. Guedes-Garcia, Vicenta Millan, Anke Becker, Marta Robledo, Jose I. Jimenez-Zurdo
Summary: The N status transduced by the NtrBC system is an important signaling cue in the root nodule endosymbiosis, and the sRNA NfeR1 influences the symbiotic performance of Sinorhizobium meliloti by fine-tuning NtrBC output.
Article
Microbiology
Laura Baxter, Proyash Roy, Emma Picot, Jess Watts, Alex Jones, Helen Wilkinson, Patrick Schaefer, Miriam Gifford, Beatriz Lagunas
Summary: The improved and complete genome sequence of Sinorhizobium (Ensifer) meliloti strain WSM1022, a microsymbiont of Medicago species, was generated using Illumina and Oxford nanopore sequencing technologies. Comparison with other rhizobial strains revealed significant genomic differences, with similarities observed between meliloti strains and high efficiency strains WSM1022 and WSM419. The high-quality rhizobial genome sequence will help unravel the features that make a rhizobial symbiont highly efficient for nitrogen fixation.
Article
Biotechnology & Applied Microbiology
Prithwi Ghosh, Katie N. Adolphsen, Svetlana N. Yurgel, Michael L. Kahn
Summary: It was found that by introducing specific genes from S. medicae into the S. meliloti strain, the symbiotic effect between rhizobia and plants could be enhanced, promoting plant growth and increasing biomass. These genes related to symbiotic effectiveness may play important roles among different rhizobial strains and could potentially provide new contributions to symbiotic relationships.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Laura Werel, Neda Farmani, Elizaveta Krol, Javier Serrania, Lars-Oliver Essen, Anke Becker
Summary: In bacteria, the receptor proteins of cAMP and cGMP are found among transcription factors of the Crp-Fnr superfamily. The prototypic Escherichia coli CAP can bind cAMP and cGMP, whereas Sinorhizobium meliloti Clr can activate transcription with both cyclic nucleotides. Crystal structures of Clr-cAMP and Clr-cGMP bound to the DNA binding site reveal similar active conformations induced by the cyclic nucleotides. Clr shows similar affinities for cAMP and cGMP in the presence of DNA, but different affinities in their absence.
Article
Agronomy
Victoria Spartakovna Muntyan, Marina Lvovna Roumiantseva
Summary: This study analyzed the complete genome sequencing data of 26 Sinorhizobium meliloti strains and 25 bacterial strains belonging to 17 genera to investigate the salt-tolerance-related genes. It was found that multiple copies of the genes associated with the first and second stages of salt stress response were present on the pSymA replicon, which is known for its role in nitrogen fixation activity. The closest phylogenetic relatives of the tested genes were identified, providing insights into the evolutionary pathways of stress-related gene pool formation in root nodule nitrogen-fixing bacteria.
Article
Microbiology
Eukene O. O. Bensig, Cecilio Valadez-Cano, ZiYu Kuang, Isabela R. R. Freire, Adrian Reyes-Prieto, Shawn R. R. MacLellan
Summary: This study identified a new stress resistance gene, srlA, in the nitrogen-fixing bacterium Sinorhizobium (Ensifer) meliloti. The gene regulates salt tolerance and oxidative stress sensitivity, influencing bacterial growth and survival under certain stress-inducing conditions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Maria E. Vladimirova, Victoria S. Muntyan, Alexey M. Afonin, Alexey N. Muntyan, Olga A. Baturina, Elena A. Dzuybenko, Alla S. Saksaganskaya, Boris Simarov, Marina L. Roumiantseva, Marsel R. Kabilov
Summary: This article reports the complete genome sequence of a symbiotic bacterial species, Sinorhizobium meliloti strain AK76, which forms nitrogen-fixing nodules on the roots of wild diploid Medicago plants grown in the Mugodgary Mountain region of Kazakhstan.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Biochemistry & Molecular Biology
Kerry S. Williamson, Mensur Dlakic, Tatsuya Akiyama, Michael J. Franklin
Summary: The bacterial heat-shock response is regulated by the alternative sigma factor, sigma(32) (RpoH), which directs the RNA polymerase to the promoters for genes required for protein refolding or degradation. In P. aeruginosa, RpoH is essential for viability and regulates genes necessary for its growth. Transcriptomics analysis revealed that increased RpoH levels induce genes involved in protein repair, damaged nucleic acid repair, amino acid metabolism, and potentially antibiotic resistances and non-ribosomal peptide synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Songli Yuan, Danxia Ke, Bo Liu, Mengke Zhang, Xiangyong Li, Haifeng Chen, Chanjuan Zhang, Yi Huang, Shuai Sun, Jiafang Shen, Shuqi Yang, Shunxin Zhou, Piao Leng, Yuefeng Guan, Xinan Zhou
Summary: This study identifies 13 novel proteins associated with Nod factor receptors and describes a potential interacting protein, GmBI-1a. GmBI-1a has a strong positive correlation with GmNFR1a and its expression is enhanced by rhizobial infection. Overexpression of GmBI-1a promotes nodule formation, while down-regulation reduces nodule number. The study provides new insights into the regulatory mechanism of GmNFR1a in the symbiotic process.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biotechnology & Applied Microbiology
Zhanwu Yang, Hui Du, Xinzhu Xing, Wenlong Li, Youbin Kong, Xihuan Li, Caiying Zhang
Summary: This study identified an important sHSP protein GmHSP17.9 in soybean nodules, revealing its significant role in nodule development and nitrogen fixation. GmHSP17.9 interacts with GmNOD100, influencing the function of soybean nodules.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Zhanwu Yang, Hui Du, Jingyi Sun, Xinzhu Xing, Youbin Kong, Wenlong Li, Xihuan Li, Caiying Zhang
Summary: GmHSP17.1 plays an important role in soybean nodulation by interacting with GmRIP1, regulating nodule development, nitrogen fixation activity, peroxidase activity, and reactive oxygen species levels. These findings are crucial for studying the mechanism of nitrogen fixation and genetic improvement of legume plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Shintaro Hara, Takashi Morikawa, Sawa Wasai, Yasuhiro Kasahara, Taichi Koshiba, Kiyoshi Yamazaki, Toru Fujiwara, Tsuyoshi Tokunaga, Kiwamu Minamisawa
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Soil Science
Ryo Shinoda, Zhihua Bao, Kiwamu Minamisawa
SOIL BIOLOGY & BIOCHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Masayuki Sugawara, Yosuke Umehara, Akito Kaga, Masaki Hayashi, Masao Ishimoto, Shusei Sato, Hisayuki Mitsui, Kiwamu Minamisawa
Review
Microbiology
Cristina Sanchez, Kiwamu Minamisawa
FRONTIERS IN MICROBIOLOGY
(2019)
Editorial Material
Plant Sciences
Brett James Ferguson, Kiwamu Minamisawa, Nacira Belen Munoz, Hon-Ming Lam
FRONTIERS IN PLANT SCIENCE
(2019)
Review
Plant Sciences
Tadakatsu Yoneyama, Junko Terakado-Tonooka, Zhihua Bao, Kiwamu Minamisawa
Article
Microbiology
Sawa Wasai-Hara, Kiwamu Minamisawa, Sylvie Cloutier, Eden S. P. Bromfield
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2020)
Article
Ecology
Haruka Arashida, Haruka Odake, Masayuki Sugawara, Ryota Noda, Kaori Kakizaki, Satoshi Ohkubo, Hisayuki Mitsui, Shusei Sato, Kiwamu Minamisawa
Summary: Symbiosis between organisms influences evolution through adaptive changes in genome architectures. Interactions between soybeans and Bradyrhizobium involve IS-mediated duplications and deletions on symbiosis islands, with host plants selecting mutualistic variants from the pool of rhizobial populations. Homologous recombination between direct IS copies provides a natural mechanism for generating deletions and duplications in symbiosis islands.
Article
Microbiology
Shintaro Hara, Naohisa Wada, Sliver Sung-Yun Hsiao, Meng Zhang, Zhihua Bao, Yoshiyuki Iizuka, Der-Chuen Lee, Shusei Sato, Sen-Lin Tang, Kiwamu Minamisawa
Summary: This study demonstrates that type II methanotrophs in the root tissues of paddy rice perform methane oxidation and nitrogen fixation simultaneously at the single-cell level. The findings provide direct evidence of the activities of methanotrophs in root tissues, suggesting that they may provide fixed nitrogen to root systems or depend on different microenvironmental niches.
Review
Biochemistry & Molecular Biology
Kiwamu Minamisawa
Summary: This mini-review highlights the potential of bacterial nitrogen fixation to mitigate greenhouse gas emissions in soybean and rice fields. It discusses the role of rhizobia in reducing nitrous oxide emissions and the use of methane-oxidizing bacteria to fix nitrogen and reduce methane emissions. By utilizing symbiotic nitrogen fixation, it is possible to mitigate GHG emissions while supporting sustainable agriculture.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Kazuyuki Okazaki, Hirohito Tsurumaru, Megumi Hashimoto, Hiroyuki Takahashi, Takashi Okubo, Takuji Ohwada, Kiwamu Minamisawa, Seishi Ikeda
Summary: The study showed distinct differences in bacterial community structures between above-ground and below-ground tissues of sugar beet, with above-ground tissues being more tightly clustered. By analyzing 16S rRNA gene sequence data of clone libraries and isolate collections, 44 potential plant growth-promoting bacteria were identified, with eight isolates demonstrating growth-promoting effects on sugar beet seedlings in inoculation tests. Seven genera were newly identified as PGPBs for sugar beet at the genus level, extending the potential for developing novel biofertilizers.
MICROBES AND ENVIRONMENTS
(2021)
Article
Biotechnology & Applied Microbiology
Arthur Fernandes Siqueira, Masayuki Sugawara, Haruka Arashida, Kiwamu Minamisawa, Cristina Sanchez
MICROBES AND ENVIRONMENTS
(2020)
Article
Biotechnology & Applied Microbiology
Sawa Wasai-Hara, Shintaro Hara, Takashi Morikawa, Masayuki Sugawara, Hideto Takami, Junich Yoneda, Tsuyoshi Tokunaga, Kiwamu Minamisawa
MICROBES AND ENVIRONMENTS
(2020)
Article
Biotechnology & Applied Microbiology
Shintaro Hara, Masatoshi Matsuda, Kiwamu Minamisawa
MICROBES AND ENVIRONMENTS
(2019)
Article
Biotechnology & Applied Microbiology
Cristina Sanchez, Arthur Fernandes Siqueira, Hisayuki Mitsui, Kiwamu Minamisawa
MICROBES AND ENVIRONMENTS
(2019)