期刊
METHODS
卷 117, 期 -, 页码 35-47出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.11.012
关键词
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资金
- Institut National Du Cancer (Melanoma PAIR program)
- Ouest Valorisation
- Ligue Nationale Contre le Cancer (LNCC et comites Grand-Ouest)
- Region Bretagne
- University of Rennes 1
- CNRS
- European Organisation for Research and Treatment of Cancer Tournesol program
- Societe Francaise de Dermatologie
- Ligue Nationale Contre le Cancer [CD-22]
- Faculty of Pharmacy of Rennes
Targeting RNAs appears as an important opportunity to modulate biological processes. Here, we overviewed critical parameters implied in RNAs competition to bind small RNAs. These competitions influence small RNA availability and thereby gene expression and cell fate. We focused on the ability of RNAs to sequester small RNA, mainly the microRNAs (miRNAs) and proposed experimental workflows to demonstrate the existence and activity of RNA-sponge. From this basic science, we detailed tailored oligonucleotides, developed to challenge the binding of small RNA. In vitro and in vivo, these tailored oligonucleotides efficiently restore small RNA activity by preventing their sequestration on RNA-sponges. (C) 2016 Elsevier Inc. All rights reserved.
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